Ribonucleotides Covalently Linked to Deoxyribonucleic Acid in T4 Bacteriophage

Speyer, Joseph F.; Chao, Julie; Chao, Lee

doi:10.1128/jvi.10.5.902-908.1972

Cited by 30 publications

(4 citation statements)

References 41 publications

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“…The isolation of covalently closed superhelical DNA containing stretches of RNA suggests that preformed pieces of RNA and DNA can be joined to one another. Chloroplast DNA (Kolodner et al, 1975) and the DNAs of both prokaryotic (Buckley et al, 1972;Speyer et al, 1972;Marmur et al, 1972) and eukaryotic (Gordin et al, 1973;Eliasson et al, 1974) viruses and of eukaryotic cells (Sato et al, 1972) also contain stretches of RNA.…”

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confidence: 99%

L cell DNA ligase joins RNA to DNA on a DNA template

Bedows¹,

Wachsman²,

Gumport³

1977

Biochemistry

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L cell DNA ligase catalyzes a covalent linkage between 5'-hosphoryl oligodeoxyribonucleotides and 3'-hydroxyl oligoribonucleotides on a complementary polydeoxyribonucleotide template. This reaction occurs to a substantially lesser extent than does the sealing of DNA to DNA. The joining of [5'32P]d(pA)12-18 to (Ap)11A on poly[d(T)] or of [5'-32P]d(pG)12-18 to 5'-hydroxyl, 3'-hydroxyl oligo(I) ON POLY[D(C)] was demonstrated by the formation of alkaline phosphatase resistant radioactivity. The 32P of the hybrid reaction products became sensitive to the action of alkaline phosphatase after treatment with alkali. Furthermore, hydrolysis of the products of the linkage of [5'-32P]d(pG)12-18 to 5'-hydroxyl, 3'-hydroxyl oligo(I) on poly[d(C)] with micrococcal nuclease and spleen phosphodiesterase resulted in the formation of [3'-32P]IMP. Attempts to seal [5'-32p[-(pA)12 to d(Ap)11-17A on poly[d(T) or [5'-32P]oligo(pI) to d(Gp)11-17G on poly[d(C)] were unsuccessful.

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confidence: 99%

L cell DNA ligase joins RNA to DNA on a DNA template

Bedows¹,

Wachsman²,

Gumport³

1977

Biochemistry

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“…Ribonucleotides that are covalently linked to DNA have been detected in several procaryotic and eucaryotic systems (1,28,32). In the cases of chloramphenicol-amplified ColEl plasmid DNA, mitochondrial DNA, and cauliflower mosaic viral DNA, ribonucleotides have been demonstrated to be linked to DNA internally (11, 18; D. G. Blair, D. J. Sherratt, D. B. Clewell, and D. R. Helinski, Fed.…”

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confidence: 99%

Ribonucleotides in unintegrated linear spleen necrosis virus DNA

Chen¹,

Temin²

1980

J Virol

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The structure of unintegrated spleen necrosis virus DNA was characterized by using various chemical and enzymatic treatments in conjunction with denaturing gels and nucleic acid hybridization probes. Throughout the course of the viral infection, the predominant species of viral DNA was that of a linear double-stranded molecule containing ribonucleotides covalently joined to the DNA. The majority of both - and + strands were continuous. The ribonucleotide linkages appeared to be relatively short, and the base composition and distribution of the ribonucleotide linkages were heterogeneous. On the average, the - strand had fewer of the ribonucleotide linkages than did the strand. Viral DNA containing ribonucleotide linkages was infectious in DNA transfection assays. The structure of spleen necrosis virus DNA was different from that of Schmidt-Ruppin Rous sarcoma virus-D, and mixed infections demonstrated that the observed differences are a result of cis-acting functions.

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“…The presence of unusual bases has not been reported (3,9,12). Recently, the presence of RNA in the genetic material of bacteriophages T4 (19) and T5 (16) was demonstrated. In the latter paper the possible relationship between the presence of RNA stretches in the DNA backbone and DNA fragmentation under alkaline conditions (1) was stressed.…”

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Ribonucleotides Linked to DNA of Herpes Simplex Virus Type 1

Hirsch¹,

Vonka²

1974

J Virol

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Cells of a continuous cell line derived from rabbit embryo fibroblasts were infected with herpes simplex type 1 virus (HSV-1) and maintained in the presence of either [5-3H]uridine or [methyl-3H]thymidine or 32PO43-. Nucleocapsids were isolated from the cytoplasmic fraction, partially purified, and treated with DNase and RNase. From the pelleted nucleocapsids, DNA was extracted and purified by centrifugation in sucrose and cesium sulfate gradients. The acid-precipitable radioactivity of [5-3H]uridine-labeled DNA was partially susceptible to pancreatic RNase and alkaline treatment; the susceptibility to the enzyme decreased with increasing salt concentration. No drop of activity of DNA labeled with [3H]thymidine was observed either after RNase or alkali treatment. Base composition analysis of [5-3H]uridine-labeled DNA showed that the radioactivity was recovered as uracil and cytosine. In the cesium sulfate gradient, the purified [5-3H]uridine-labeled DNA banded at the same position as the 32P-labeled DNA. The present data tend to suggest that ribonucleotide sequences are present in HSV DNA, that they are covalently attached to the viral DNA, and that they can form double-stranded structures.

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Ribonucleotides Covalently Linked to Deoxyribonucleic Acid in T4 Bacteriophage

Cited by 30 publications

References 41 publications

L cell DNA ligase joins RNA to DNA on a DNA template

L cell DNA ligase joins RNA to DNA on a DNA template

Ribonucleotides in unintegrated linear spleen necrosis virus DNA

Ribonucleotides Linked to DNA of Herpes Simplex Virus Type 1

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