Ribosomal and protein coding gene based multigene phylogeny on the family Streptomycetaceae

Han, Ji-Hye; Cho, Myoungho; Kim, Seung Bum

doi:10.1016/j.syapm.2011.08.007

Cited by 22 publications

(21 citation statements)

References 18 publications

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“…Therefore, deduced amino acids, in addition to DNA sequences, can be used for the delineation of groups or species within the genus Streptomyces. Many researchers have been used rpoB gene analysis for the confirm identification of the family Streptomycetaceae [21,35].…”

Section: Resultsmentioning

confidence: 99%

Degradation of natural rubber latex by new Streptomyces labedae strain ASU-03 isolated from Egyptian soil

et al. 2015

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Natural rubber latex is one of the problems that raises the environmental concerns. In this study the degrading ability of Ficus elastica rubber latex by a bacterium strain ASU 03, isolated from Egyptian soil was assessed. The strain was able to produce clear zone around its colony on latex rubber containing medium and was identified by conventional methods as Streptomyces sp. Phylogenetic analysis of 16S rRNA (16S rRNA) and RNA polymerase β subunit (rpoB) genes were applied. Results of the 16S rRNA gene analysis revealed that the strain was highly related to Streptomyces sp. (100% similarity), so the rpoB gene was partially sequenced to clarify the specific name of the isolate. Phylogenetic tree based on rpoB gene sequences indicated that strain ASU 03 was highly similar to the reference strain Streptomyces labedae and both were shared a one cluster. The current results demonstrated that the use of a rpoB gene based method gives a better resolution in the species level identification. To our knowledge, this species has never been reported to be involved in natural rubber degradation. This was therefore the first report about the degradation of Ficus elastic by S. labedae. The degradation of Ficus elastica rubber latex was determined by measuring the increase in protein content of bacterium (mg/g dry wt), reduction in molecular weight (g/mol) and inherent viscosity (dL/g) of the latex. Moreover the degradation was also confirmed by formation of aldehyde or keto group by Schiff's reagent and by observing the growth of the Streptomyces strain using scanning electron microscopy.

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Section: Resultsmentioning

confidence: 99%

Degradation of natural rubber latex by new Streptomyces labedae strain ASU-03 isolated from Egyptian soil

et al. 2015

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“…This might suggest that there is a potential for the occurrence of horizontal gene transfer events in the rRNA genes [87,90] . Hence, phylogenetic construction based on 16S rRNA gene sequences could not assure well-resolved phylogenetic trees that represent relationship between bacterial species particularly within genus Streptomyces [9,12,51] . The 16S rRNA gene sequences provide low resolution as phylogenetic marker for Streptomyces species.…”

Section: Ribosomal Rna Genes -16s Rrna Genementioning

confidence: 99%

“…The 23S rRNA gene is formerly less preferred to be used for phylogenetic study as compared to 16S rRNA gene due to costing consideration [9] . However, the use of 23S rRNA gene in phylogenetic analysis is eventually being highlighted because of several factors including reduced sequencing costs due to the advancement of technology such as next generation DNA sequencing [92] .…”

Section: Ribosomal Rna Genes -23s Rrna Genementioning

confidence: 99%

“…As a matter of fact, Streptomyces consists of multicellular, Gram positive, filamentous aerobic, and mycelial bacteria that live mainly in soil as saprophytes [7] . These bacteria have special features such as complex life cycle involving the development of substrate mycelium with extensive branches containing LL-diaminopimelic acid as main diamino acid and carries aerial hyphae that are able to differentiate into spores or arthrospores [8,9] . Spores allow extended survival of Streptomyces species in an inactive form in the soil because they are known to be resistant to both water and nutrients deficiencies as well as high temperature in unfavourable environments [7] .…”

Section: Introductionmentioning

confidence: 99%

“…The genus Streptomyces within the order Actinomycetales has spectacular diversity in morphology, genomic size, genomic G+C content, and amount of coding sequences. Streptomyces bacteria are noted by their large and linearized chromosomes with approximately 8.5-12 Mb in size and high GC content of about 67-78% [3,9,13] . The large genome of Streptomyces could account for their ability to produce many secondary metabolites [14] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Taxonomic and Characterization Methods of Streptomyces: A Review

Law¹,

Tan²,

Wong³

et al. 2018

Prog Micobes Mol Biol

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Streptomycetaceae: Phylogeny, Ecology and Pathogenicity

Glaeser

Kämpfer

2016

Encyclopedia of Life Sciences

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The family Streptomycetaceae contains three genera, Streptomyces , Kitasatospora and Streptacidiphilus , with Streptomyces being the best known and most diverse of these three genera. Members of this family are adapted to a wide range of environmental conditions and habitats, and show a variety of colony – and cell morphological characteristics. Streptomyces are notable for their complex developmental cycle and production of bioactive secondary metabolites, producing more than two‐thirds of commercially available and clinically useful antibiotics of natural origin (e.g. neomycin, cypemycin, grisemycin, bottromycins and chloramphenicol). Antibacterial, antifungal, antiparasitic and immunosuppressant compounds have all been identified as products of Streptomyces secondary metabolism. Isolated predominantly from soil and decaying vegetation, most streptomycetes produce spores. Many of them produce a distinct ‘earthy’ odour that results from production of a volatile metabolite, geosmin. They are also capable of forming many and diverse hydrolytic exoenzymes, like cellulases and chitinases. The genetics and genomics of streptomycetes is a rapidly developing area. Only a few Streptomyces species are known to be pathogens, although infections, in humans, such as mycetoma, can be caused by S. somaliensis and S. sudanensis , and in plants can be caused by S. caviscabies , S. acidiscabies , S. turgidiscabies and S. scabies . Key Concepts Comparisons of whole genome sequences will become the future basis of classification of Streptomyces species. Morphological and physiological features as well as chemotaxonomic markers remain distinctive and important for the taxonomy of the family Streptomycetaceae. Genetics and Genomics of streptomycetes reveal complex mechanisms of adaptation to various environments. Antimicrobial compounds and other secondary metabolites are produced in natural habitats inter‐kingdom communication, as signal molecules or defence molecules to defend a growth niche.

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Ribosomal and protein coding gene based multigene phylogeny on the family Streptomycetaceae

Cited by 22 publications

References 18 publications

Degradation of natural rubber latex by new Streptomyces labedae strain ASU-03 isolated from Egyptian soil

Degradation of natural rubber latex by new Streptomyces labedae strain ASU-03 isolated from Egyptian soil

Taxonomic and Characterization Methods of Streptomyces: A Review

Streptomycetaceae: Phylogeny, Ecology and Pathogenicity

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