Ribosomal DNA loci derived from<i>Brachypodium stacei</i>are switched off for major parts of the life cycle of<i>Brachypodium hybridum</i>

Borowska-Zuchowska, Natalia; Robaszkiewicz, Ewa; Wolny, Elżbieta; Betekhtin, Alexander; Hasterok, Robert

doi:10.1093/jxb/ery425

Cited by 7 publications

(6 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The S-genome rDNA homeologs could not form the nucleolus in either of the studied genotypes as they were transcriptionally silenced. The inactive state of the aforementioned loci in ABR113 was further confirmed using the silver staining method ( Borowska-Zuchowska et al, 2019 ).…”

Section: Resultsmentioning

confidence: 90%

“…Such a specificity of the ND regulation among the floral organs in B. hybridum has not been observed yet. In addition to the current study on genotype 3-7-2, the ND establishment in the meiocytes was only analyzed in the reference genotype ABR113 ( Borowska-Zuchowska et al, 2019 ). The S-genome rDNA homeologs could not form the nucleolus in either of the studied genotypes as they were transcriptionally silenced.…”

Section: Resultsmentioning

confidence: 99%

“…By contrast, the S-genome loci were usually located at the nuclear periphery and were enriched in the heterochromatic H3K9me2 mark ( Borowska-Zuchowska and Hasterok, 2017 ). Studies on the developmental regulation in ABR113 did not reveal any stage in B. hybridum ontogenesis in which the ND is abolished ( Borowska-Zuchowska et al, 2016 , 2019 ). We recently observed a reduction in the S-genome-like 35S rDNA copy number in 16 B. hybridum genotypes.…”

Section: Introductionmentioning

confidence: 89%

“…FISH on the polyacrylamide pads was performed as was described in Borowska-Zuchowska et al (2019) . A pre-denatured hybridization mixture with 25S rDNA as the probe was applied to the pads, and they were denatured together at 75°C for 8 min.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity in Brachypodium hybridum

et al. 2021

Self Cite

View full text Add to dashboard Cite

Nucleolar dominance (ND) is an epigenetic, developmentally regulated phenomenon that describes the selective inactivation of 35S rDNA loci derived from one progenitor of a hybrid or allopolyploid. The presence of ND was documented in an allotetraploid grass, Brachypodium hybridum (genome composition DDSS), which is a polyphyletic species that arose from crosses between two putative ancestors that resembled the modern B. distachyon (DD) and B. stacei (SS). In this work, we investigated the developmental stability of ND in B. hybridum genotype 3-7-2 and compared it with the reference genotype ABR113. We addressed the question of whether the ND is established in generative tissues such as pollen mother cells (PMC). We examined condensation of rDNA chromatin by fluorescence in situ hybridization employing state-of-art confocal microscopy. The transcription of rDNA homeologs was determined by reverse-transcription cleaved amplified polymorphic sequence analysis. In ABR113, the ND was stable in all tissues analyzed (primary and adventitious root, leaf, and spikes). In contrast, the 3-7-2 individuals showed a strong upregulation of the S-genome units in adventitious roots but not in other tissues. Microscopic analysis of the 3-7-2 PMCs revealed extensive decondensation of the D-genome loci and their association with the nucleolus in meiosis. As opposed, the S-genome loci were always highly condensed and localized outside the nucleolus. These results indicate that genotype-specific loss of ND in B. hybridum occurs probably after fertilization during developmental processes. This finding supports our view that B. hybridum is an attractive model to study ND in grasses.

show abstract

Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity in Brachypodium hybridum

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the development of DNA:DNA in situ hybridisation (ISH) brought cytogenetics studies to the next level and tightly linked them with molecular biology [ 6 ]. ISH enabled the physical mapping of specific DNA sequences on both chromosomes and interphase nuclei, and indeed, the position of the DNA sequence of choice can be traced throughout the entire cell cycle [ 7 ]. Hence, comparative cytogenetics has obtained new, advanced tools for plant evolutionary studies [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Tracing the Evolution of the Angiosperm Genome from the Cytogenetic Point of View

Borowska-Zuchowska

Senderowicz

Trunova

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Cytogenetics constitutes a branch of genetics that is focused on the cellular components, especially chromosomes, in relation to heredity and genome structure, function and evolution. The use of modern cytogenetic approaches and the latest microscopes with image acquisition and processing systems enables the simultaneous two- or three-dimensional, multicolour visualisation of both single-copy and highly-repetitive sequences in the plant genome. The data that is gathered using the cytogenetic methods in the phylogenetic background enable tracing the evolution of the plant genome that involve changes in: (i) genome sizes; (ii) chromosome numbers and morphology; (iii) the content of repetitive sequences and (iv) ploidy level. Modern cytogenetic approaches such as FISH using chromosome- and genome-specific probes have been widely used in studies of the evolution of diploids and the consequences of polyploidy. Nowadays, modern cytogenetics complements analyses in other fields of cell biology and constitutes the linkage between genetics, molecular biology and genomics.

show abstract

Unique nucleolar dominance patterns in different ploidy hybrid lineages derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Liu

Zhi

et al. 2023

Aquaculture

View full text Add to dashboard Cite

Ribosomal DNA loci derived fromBrachypodium staceiare switched off for major parts of the life cycle ofBrachypodium hybridum

Abstract: Analysis of 35S rDNA activity in meiocytes, microspores, and different stages of embryo development in Brachypodium hybridum reveals that Brachypodium stacei-like loci are switched off.

Cited by 7 publications

References 61 publications

To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity in Brachypodium hybridum

To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity in Brachypodium hybridum

Tracing the Evolution of the Angiosperm Genome from the Cytogenetic Point of View

Unique nucleolar dominance patterns in different ploidy hybrid lineages derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Contact Info

Product

Resources

About