Ribosomal DNA sequences of Glugea anomala, G. stephani, G. americanus and Spraguea lophii (Microsporidia):phylogenetic reconstruction

Pomport-Castillon, C.; Jonckheere, Johan F. De; Romestand, Bernard

doi:10.3354/dao040125

Cited by 21 publications

(14 citation statements)

References 9 publications

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“…Our results support those of Pomport-Castillon et al (2000), who hypothesized that G. anomala, G. stephani, and G. atherinae may in fact be only 1 species of the genus Glugea based on sequence homology. Evidence for G. hertwigi being the same species as G. anomala is also supported by suc- senberg 1968).…”

Section: Discussionsupporting

confidence: 91%

“…Although G. hert-wigi has been documented in smelt populations throughout Europe and the Atlantic, morphological and molecular data on the parasite are currently lacking. Microsporidia in the genus Glugea have been considered problematic; however, it has been demonstrated that G. anomala and G. stephani are likely the same species, whereas G. americanus belongs in fact to the genus Spraguea (Pomport-Castillon et al 2000, Freeman et al 2004. In the present study, G. hertwigi from O. mordax is characterized with transmission electron microscopy (TEM) and ribosomal DNA (rDNA) sequences to determine its relatedness within the genus Glugea.…”

Section: Introductionmentioning

confidence: 90%

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Phylogeny and morphology of Glugea hertwigi from rainbow smelt Osmerus mordax found in Prince Edward Island, Canada

Lovy¹,

Kostka²,

Dyková³

et al. 2009

Dis. Aquat. Org.

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Infection of rainbow smelt Osmerus mordax with the microsporidian Glugea hertwigi was diagnosed for the first time in Prince Edward Island, Canada. The prevalence of infection was 24%, 45 infected out of 187 examined fish captured in February and March 2009. Both large and small xenomas of G. hertwigi observed within the submucosa of the gastrointestinal tract and along the mesentery of the host contained only mature spores. Advanced and degraded xenomas associated with host reaction were described using light and transmission electron microscopy. The first rDNA sequence of G. hertwigi prepared in the present study completed the set of sequences of Glugea spp. available for comparison. The high level of rDNA sequence identity between Glugea spp. suggests that these may be variants of a single species.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 90%

Phylogeny and morphology of Glugea hertwigi from rainbow smelt Osmerus mordax found in Prince Edward Island, Canada

Lovy¹,

Kostka²,

Dyková³

et al. 2009

Dis. Aquat. Org.

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“…The authors agree with the earlier findings of Pomport-Castillon et al (2000), Nilsen (2000) and Lom and Nilsen (2003) that the parasite from the American anglerfish, described as Glugea americanus (Takvorian and Cali 1986), should be transferred to the genus Spraguea as Spraguea americana comb. n. and the description of the genus Spraguea be amended to include such monomorphic forms.…”

supporting

confidence: 80%

“…They also commented upon the fact that the dimorphic condition was always present but to varying degrees within given xenomas, a condition also observed by Weissenberg (1976) and considered by him to be due to xenoma maturation. Assumptions can be made that during the SSU sequencing of Spraguea lophii from European lophiids by Nilsen (1999) and Pomport-Castillon et al (2000), the presence of two species of microsporidians would have become apparent, therefore the dimorphic infection in European lophiids must be considered to be caused by a single species of microsporidian. As this dimorphic condition is never present in the Japanese and American anglerfish species, but is a consistent feature in the European species, the microsporidia from both the Japanese and American anglerfish species should be currently considered as a separate species from S. lophii infecting European anglerfish despite having an almost identical SSU rRNA gene sequence.…”

Section: Discussionmentioning

confidence: 99%

A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

Freeman

Yokoyama²,

Ogawa³

2004

FOLIA PARASIT

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Abstract. In the present study, a high percentage of Japanese anglerfish, Lophius litulon (Jordan, 1902), contained a microsporidian infection of the nervous tissues. Xenomas were removed and prepared for standard wax histology and transmission electron microscopy (TEM). DNA extractions were performed on parasite spores and used in PCR and sequencing reactions. Fresh spores measured 3.4 × 1.8 µm and were uniform in size with no dimorphism observed. TEM confirmed that only a single developmental cycle and a single spore form were present. Small subunit (SSU) rDNA sequences were >99.5% similar to those of Spraguea lophii (Doflein, 1898) and Glugea americanus (Takvorian et Cali, 1986) from the European and American Lophius spp. respectively. The microsporidian from the nervous tissue of L. litulon undoubtedly belongs in the genus Spraguea Sprague et Vávra, 1976 and the authors suggest a revision to the generic description of Spraguea to include monomorphic forms and the transfer of Glugea americanus to Spraguea americana comb. n. Since no major differences in ultrastructure or SSU rDNA sequence data exist between Spraguea americana and the microsporidian from the Japanese anglerfish, they evidently belong to the same species. This report of Spraguea americana is the first report of a Spraguea species from L. litulon and indeed from the Pacific water mass.

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“…In a similar manner to L. salmonae and H. sutherlandae, G. anomala, G. hertwigi, and G. stephani have been reported from multiple fish hosts (smelts and sticklebacks) from the northern USA and Canada, including areas that overlap the range of sculpins (Scott & Crossman 1973, Becker 1983, Lovy et al 2009). Overall, the variation in these Glugea rDNA sequences could indicate that these parasites represent local isolates of a single species, as has been suggested by multiple authors (Canning & Lom 1986, Pomport-Castillon et al 2000, Lovy et al 2009, Liu et al 2013). Both environment (e.g.…”

Section: Discussionsupporting

confidence: 52%

Distribution, prevalence, and pathology of a microsporidian infecting freshwater sculpins

Ryan

Kohler²

2016

Dis. Aquat. Org.

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Microsporidian infections are common in many fish species, yet detailed studies of these parasites in ecologically important wild populations are rare. Phylogenetic analysis using rDNA sequence data and parasite morphology indicate that mottled sculpin Cottus bairdii and slimy sculpin C. cognatus are hosts for Glugea sp. microsporidia in the northern USA. Glugea sp. is common in the Michigan populations sampled for this study, and prevalence was ≥70% in 4 of 6 infected populations (range ~4 to 80%). Glugea sp. infection causes the formation of xenomas associated with the body wall, fat body, gonads, and kidneys. Infections range from mild to very heavy, with variable xenoma numbers and sizes. Female sculpin experience heavier infections and more frequent infection of the gonads relative to males. Glugea sp. is transmitted horizontally between hosts through ingestion of spores. Vertical transmission may also be possible, either by spores infecting eggs directly or by spores contaminating the surface of eggs in the ovary or in the nest. The frequency and route of vertical transmission requires further study, but if it occurs, it may partly explain the high prevalence of infection. Our study combined with previous research suggests that additional molecular data and cross-infection experiments should be conducted to clarify species designations in the genus Glugea.

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Ribosomal DNA sequences of Glugea anomala, G. stephani, G. americanus and Spraguea lophii (Microsporidia):phylogenetic reconstruction

Cited by 21 publications

References 9 publications

Phylogeny and morphology of Glugea hertwigi from rainbow smelt Osmerus mordax found in Prince Edward Island, Canada

Phylogeny and morphology of Glugea hertwigi from rainbow smelt Osmerus mordax found in Prince Edward Island, Canada

A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

Distribution, prevalence, and pathology of a microsporidian infecting freshwater sculpins

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