Ribosomal protein L19 overexpression activates the unfolded protein response and sensitizes MCF7 breast cancer cells to endoplasmic reticulum stress-induced cell death

Hong, Mina; Kim, Hyung-ryong; Kim, Inki

doi:10.1016/j.bbrc.2014.06.036

Cited by 39 publications

(31 citation statements)

References 14 publications

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“…(Adams et al, 1992;Finak et al, 2008;Atsuta et al, 2002;Hannemann et al, 2006;Hong et al, 2014;Wang et al, 2010;Wilson-Edell et al, 2014) L22, L41 Downregulation Osteosarcoma S3, S15a Upregulation Low expression of L7a predicts poor survival of osteosarcoma patients with lung metastasis.…”

Section: Rps As Potential Cancer Biomarkersmentioning

confidence: 99%

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Xiong

Sun

2016

Sci. China Life Sci.

109

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Ribosomal proteins (RPs), the essential components of the ribosome, are a family of RNA-binding proteins, which play prime roles in ribosome biogenesis and protein translation. Recent studies revealed that RPs have additional extra-ribosomal functions, independent of protein biosynthesis, in regulation of diverse cellular processes. Here, we review recent advances in our understanding of how RPs regulate apoptosis, cell cycle arrest, cell proliferation, neoplastic transformation, cell migration and invasion, and tumorigenesis through both MDM2/p53-dependent and p53-independent mechanisms. We also discuss the roles of RPs in the maintenance of genome integrity via modulating DNA damage response and repair. We further discuss mutations or deletions at the somatic or germline levels of some RPs in human cancers as well as in patients of Diamond-Blackfan anemia and 5q-syndrome with high susceptibility to cancer development. Moreover, we discuss the potential clinical application, based upon abnormal levels of RPs, in biomarker development for early diagnosis and/or prognosis of certain human cancers. Finally, we discuss the pressing issues in the field as future perspectives for better understanding the roles of RPs in human cancers to eventually benefit human health. ribosomal protein, tumorigenesis, genomic integrity, ribosomal stress, p53, MDM2 Citation:Xu, X., Xiong, X., and Sun, Y. (2016). The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity. Sci China Life Sci 59, 656 -672.

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Section: Rps As Potential Cancer Biomarkersmentioning

confidence: 99%

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Xiong

Sun

2016

Sci. China Life Sci.

109

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“…CNVs in RPL19 and RPL23 in breast cancer ( Table 2) likely occur due to their co-amplification with ERBB2 on 17q12. Overexpression of RPL19 has previously been described in a subset of breast cancers (Hong et al 2014). The small cluster of 144 tumors that did not group according to tissue of origin ( Supplementary Fig.…”

Section: Discussionmentioning

confidence: 82%

“…For example, RPL3 regulates chemotherapy response in certain lung and colon cancers, associates with the high-risk neuroblastoma subtype, and may have a role in the acquisition of lung cancer multidrug resistance (Khan et al 2015;Russo et al 2016;. Breast cancers with elevated expression of RPL19 are more sensitive to apoptosis mediated drugs that induce endoplasmic reticulum stress (Hong et al 2014). RPS11 and RPS20…”

Section: Discussionmentioning

confidence: 99%

“…Perturbations of RP expression have been found in numerous human cancers, including those of the breast, pancreas, bladder, brain and many other tissues (Lai and Xu 2007;Artero-Castro et al 2011;Jung et al 2011;Hong et al 2014;Kardos et al 2014;Khan et al 2015;Paquet et al 2015;Yong et al 2015;Russo et al 2016;Sim et al 2016;Fan et al 2017;Shi et al 2017). Mutations and deletions of RP-encoding genes have also been found in endometrial cancer, colorectal cancer, glioma, and various hematopoietic malignancies (Goudarzi and Lindstrom 2016;Ajore et al 2017;Fancello et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic and Prognostic Implications of Ribosomal Protein Transcript Coordination in Human Cancers

Dash

2017

Preprint

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Ribosomes, the organelles responsible for the translation of mRNA, are comprised of rRNA and ~80 ribosomal proteins (RPs). Although canonically assumed to be maintained in equivalent proportions, some RPs have been shown to possess differential expression across tissue types. Dysregulation of RP expression occurs in a variety of human diseases, notably in many cancers, and altered expression of some RPs correlates with different tumor phenotypes and patient survival. To investigate the impact of global RP transcript (RPT) expression patterns on tumor phenotypes, we analyzed RPT expression of ~10,000 human tumors and 700 normal tissues with t-distributed stochastic neighbor embedding (t-SNE). We show here that normal tissues and cancers possess readily discernible RPT expression patterns. In tumors, this patterning is distinct from normal tissues, distinguishes tumor subtypes from one another, and in many cases correlates with molecular, pathological, and clinical features, including survival. Collectively, RPT expression can be used as a powerful and novel method of tumor classification, offering a potential clinical tool for prognosis and therapeutic stratification.

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“…Despite the essential role of the ribosome in protein synthesis, RPL19 is predicted to have an extra-protein translational function, and its overexpression in cell culture activated the cellular signaling pathway of the UPR (unfolded protein response) (Hong et al, 2014). The UPR pathway is conserved from yeast to humans (Zhang et al, 2016) and activated in response to an overloading of unfolded or misfolded peptides, and its action can either have a protective effect, with the change of protein synthesis rate decreasing peptide load into the endoplasmic reticulum or inducing cell death with apoptotic characteristics (Kim et al, 2008;Hong et al, 2014). The RPL19 gene was upregulated in ticks fed tick-resistant cattle, which indicates that cell death was the main pathway activated to salivary gland cells during host-parasite interactions.…”

Section: Degs Between Ticks Fed On Tick-susceptible and Resistant Cattlementioning

confidence: 99%

Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts

Giachetto

Cunha

Nhani

et al. 2020

Front. Cell. Infect. Microbiol.

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The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na + /dicarboxylate, Na + /tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.

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Ribosomal protein L19 overexpression activates the unfolded protein response and sensitizes MCF7 breast cancer cells to endoplasmic reticulum stress-induced cell death

Cited by 39 publications

References 14 publications

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Diagnostic and Prognostic Implications of Ribosomal Protein Transcript Coordination in Human Cancers

Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts

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