Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance

Vemanna, Ramu S.; Dawane, Akashata; Lee, Seong-Hee; Oh, Sunhee; Lee, Hee-Kyung; Sun, Liang; Senthil‐Kumar, Muthappa; Mysore, Kirankumar S.

doi:10.1111/mpp.12991

Cited by 26 publications

(16 citation statements)

References 75 publications

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“…In a mutation study in Arabidopsis (Falcone-Ferreyra et al, 2013), the altering of three copies of the RPL10 gene resident in the genome indicated that compensation between copies occurred and expression differed between tissues. These Arabidopsis studies and studies in human (Klauck et al, 2006;reviewed in Pollutri and Penzo, 2020) and Nicotiana benthamiana (Ramu et al, 2020) are consistent with the more general model that variation in essential RPs, such as RPL10, can moderate the translation activity of ribosomes to preferentially accept certain mRNAs and/or undertake non-ribosomal level regulation of transcription and signal transduction.…”

Section: Rpl10supporting

confidence: 66%

Integrating Wheat Nucleolus Structure and Function: Variation in the Wheat Ribosomal RNA and Protein Genes

2021

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We review the coordinated production and integration of the RNA (ribosomal RNA, rRNA) and protein (ribosomal protein, RP) components of wheat cytoplasmic ribosomes in response to changes in genetic constitution, biotic and abiotic stresses. The components examined are highly conserved and identified with reference to model systems such as human, Arabidopsis, and rice, but have sufficient levels of differences in their DNA and amino acid sequences to form fingerprints or gene haplotypes that provide new markers to associate with phenotype variation. Specifically, it is argued that populations of ribosomes within a cell can comprise distinct complements of rRNA and RPs to form units with unique functionalities. The unique functionalities of ribosome populations within a cell can become central in situations of stress where they may preferentially translate mRNAs coding for proteins better suited to contributing to survival of the cell. In model systems where this concept has been developed, the engagement of initiation factors and elongation factors to account for variation in the translation machinery of the cell in response to stresses provided the precedents. The polyploid nature of wheat adds extra variation at each step of the synthesis and assembly of the rRNAs and RPs which can, as a result, potentially enhance its response to changing environments and disease threats.

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Section: Rpl10supporting

confidence: 66%

Integrating Wheat Nucleolus Structure and Function: Variation in the Wheat Ribosomal RNA and Protein Genes

2021

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“…For example, silencing the ribosomal genes RPL12 and RPL19 in the model plant Nicotiana benthamiana or Arabidopsis compromised non-host resistance to multiple bacterial pathogens 95 . Similarly, it was also found that the ribosomal protein large subunit QM/RPL10 positively regulated defense and protein- translation mechanisms for the resistance of N. benthamiana 96 . The interaction of ribosomal protein RPL12 with the receptor for activated C‐Kinase 1 ( RACK1 ) also plays a key role in innate immunity in Arabidopsis 97 , 98 .…”

Section: Discussionmentioning

confidence: 88%

Genome-wide transcriptome reveals mechanisms underlying Rlm1-mediated blackleg resistance on canola

Zhai

Liu

Song

et al. 2021

Sci Rep

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Genetic resistance to blackleg (Leptosphaeria maculans, Lm) of canola (Brassica napus, Bn) has been extensively studied, but the mechanisms underlying the host–pathogen interaction are still not well understood. Here, a comparative transcriptome analysis was performed on a resistant doubled haploid Bn line carrying the resistance gene Rlm1 following inoculation with a virulent (avrLm1) or avirulent (AvrLm1) Lm isolate on cotyledons. A total of 6999 and 3015 differentially expressed genes (DEGs) were identified, respectively, in inoculated local tissues with compatible (susceptible) and incompatible (resistant) interactions. Functional enrichment analysis found several biological processes, including protein targeting to membrane, ribosome and negative regulation of programmed cell death, were over-represented exclusively among up-regulated DEGs in the resistant reaction, whereas significant enrichment of salicylic acid (SA) and jasmonic acid (JA) pathways observed for down-regulated DEGs occurred only in the susceptible reaction. A heat-map analysis showed that both biosynthesis and signaling of SA and JA were induced more significantly in the resistant reaction, implying that a threshold level of SA and JA signaling is required for the activation of Rlm1-mediated resistance. Co-expression network analysis revealed close correlation of a gene module with the resistance, involving DEGs regulating pathogen-associated molecular pattern recognition, JA signaling and transcriptional reprogramming. Substantially fewer DEGs were identified in mock-inoculated (control) cotyledons, relative to those in inoculated local tissues, including those involved in SA pathways potentially contributing to systemic acquired resistance (SAR). Pre-inoculation of cotyledon with either an avirulent or virulent Lm isolate, however, failed to induce SAR on remote tissues of same plant despite elevated SA and PR1 protein. This study provides insights into the molecular mechanism of Rlm1-mediated resistance to blackleg.

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“…RPL10 was upregulated at early time points in resistant genotypes, however, in TN1 upregulation was at 6 dpi indicating mechanism of early sensing of stress in tolerant variety compared to sensitive genotype. Mutation/silencing of RPL10 in Arabidopsis , Nicotiana benthamiana showed susceptible phenotype and weak ABA response [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic responses under combined bacterial blight and drought stress in rice reveal potential genes to improve multi-stress tolerance

et al. 2022

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Background The unprecedented drought and frequent occurrence of pathogen infection in rice is becoming more due to climate change. Simultaneous occurrence of stresses lead to more crop loss. To cope up multiple stresses, the durable resistant cultivars needs to be developed, by identifying relevant genes from combined biotic and abiotic stress exposed plants. Results We studied the effect of drought stress, bacterial leaf blight disease causing Xanthomonas oryzae pv. oryzae (Xoo) pathogen infection and combined stress in contrasting BPT5204 and TN1 rice genotypes. Mild drought stress increased Xoo infection irrespective of the genotype. To identify relevant genes that could be used to develop multi-stress tolerant rice, RNA sequencing from individual drought, pathogen and combined stresses in contrasting genotypes has been developed. Many important genes are identified from resistant genotype and diverse group of genes are differentially expressed in contrasting genotypes under combined stress. Further, a meta-analysis from individual drought and Xoo pathogen stress from public domain data sets narrowed- down candidate differentially expressed genes. Many translation associated genes are differentially expressed suggesting their extra-ribosomal function in multi-stress adaptation. Overexpression of many of these genes showed their relevance in improving stress tolerance in rice by different scientific groups. In combined stress, many downregulated genes also showed their relevance in stress adaptation when they were over-expressed. Conclusions Our study identifies many important genes, which can be used as molecular markers and targets for genetic manipulation to develop durable resistant rice cultivars. Strategies should be developed to activate downregulated genes, to improve multi-stress tolerance in plants.

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Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance

Cited by 26 publications

References 75 publications

Integrating Wheat Nucleolus Structure and Function: Variation in the Wheat Ribosomal RNA and Protein Genes

Integrating Wheat Nucleolus Structure and Function: Variation in the Wheat Ribosomal RNA and Protein Genes

Genome-wide transcriptome reveals mechanisms underlying Rlm1-mediated blackleg resistance on canola

Transcriptomic responses under combined bacterial blight and drought stress in rice reveal potential genes to improve multi-stress tolerance

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