Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu

Pletnev, Philipp; Shulenina, Olga; Evfratov, Sergey; Treshin, Vsevolod; Subach, Maksim F; Serebryakova, Marina V.; Osterman, Ilya А.; Paleskava, Alena; Bøgdanov, A.; Dontsova, Olga А.; Konevega, Andrey L.; Сергиев, Петр В.

doi:10.1016/j.jbc.2022.101914

Cited by 14 publications

(15 citation statements)

References 35 publications

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“…coli (EcRimI), which encodes the protein N -acetyltransferase enzyme ( Figure 1 A). EcRimI acetylates a number of proteins, including ribosomal protein S18 [ 35 ], translation elongation factor Tu [ 36 ], and other proteins [ 37 ]. The human protein N -acetyltransferase Naa50, an orthologue of archaeal SNAT, shows SNAT enzyme activity [ 31 ], indicating that E .…”

Section: Resultsmentioning

confidence: 99%

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Escherichia coli RimI Encodes Serotonin N-Acetyltransferase Activity and Its Overexpression Leads to Enhanced Growth and Melatonin Biosynthesis

Lee

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2023

Biomolecules

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Serotonin N-acetyltransferase (SNAT) functions as the penultimate or final enzyme in melatonin biosynthesis, depending on the substrate. The Escherichia coli orthologue of archaeal SNAT from Thermoplasma volcanium was identified as RimI (EcRimI), with 42% amino acid similarity to archaeal SNAT. EcRimI has been reported to be an N-acetyltransferase enzyme. Here, we investigated whether EcRimI also exhibits SNAT enzyme activity. To achieve this goal, we purified recombinant EcRimI and examined its SNAT enzyme kinetics. As expected, EcRimI showed SNAT activity toward various amine substrates including serotonin and 5-methoxytryptamine, with Km and Vmax values of 531 μM and 528 pmol/min/mg protein toward serotonin and 201 μM and 587 pmol/min/mg protein toward 5-methoxytryptamine, respectively. In contrast to the rimI mutant E. coli strain that showed no growth defect, the EcRimI overexpression strain exhibited a 2-fold higher growth rate than the control strain after 24 h incubation in nutrient-rich medium. The EcRimI overexpression strain produced more melatonin than the control strain in the presence of 5-methoxytryptamine. The enhanced growth effect of EcRimI overexpression was also observed under cadmium stress. The higher growth rate associated with EcRimI expression was attributed to increased protein N-acetyltransferase activity, increased synthesis of melatonin, or the combined effects of both.

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Section: Resultsmentioning

confidence: 99%

“…The E . coli rimI mutant strain exhibits moderate growth retardation in minimal medium, but this growth difference is nonsignificant in nutrient-rich medium such as LB medium [ 36 ]. Importantly, as temperature strongly affects the solubility of the recombinant protein expressed in E .…”

Section: Resultsmentioning

confidence: 99%

Escherichia coli RimI Encodes Serotonin N-Acetyltransferase Activity and Its Overexpression Leads to Enhanced Growth and Melatonin Biosynthesis

Lee

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2023

Biomolecules

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“…4e) has multiple rimJ genes and only one rimI gene encoding a ribosomal-protein S18 alanine N-acetyltransferase in short distances with a 60% GC ratio. RimI with acetylation activity targeting two distinct objectives [ribosomal protein S18 and elongation factor Tu (EF-Tu)] was also known for high translation efficiency in E. coli, which might be also true to our strain 32 . Low GC ratios (60.0% vs. 64.7%, chromosomal backbone) and three genes linked to both transposase and resolvase, suggest a high possibility for horizontally transferred rimJ genes in region A (5 copies) (Fig.…”

Section: Isolation Of Psychrophilicmentioning

confidence: 86%

Morphological and physiological adaptations of psychrophilic Pseudarthrobacter psychrotolerans YJ56 under temperature stress

Son,

Min,

Shin

et al. 2023

Sci Rep

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Both culture-independent and culture-dependent analyses using Nanopore-based 16S rRNA sequencing showed that short-term exposure of Antarctic soils to low temperature increased biomass with lower bacterial diversity and maintained high numbers of the phylum Proteobacteria, Firmicute, and Actinobacteria including Pseudarthrobacter species. The psychrophilic Pseudarthrobacter psychrotolerans YJ56 had superior growth at 13 °C, but could not grow at 30 °C, compared to other bacteria isolated from the same Antarctic soil. Unlike a single rod-shaped cell at 13 °C, strain YJ56 at 25 °C was morphologically shifted into a filamentous bacterium with several branches. Comparative genomics of strain YJ56 with other genera in the phylum Actinobacteria indicate remarkable copy numbers of rimJ genes that are possibly involved in dual functions, acetylation of ribosomal proteins, and stabilization of ribosomes by direct binding. Our proteomic data suggested that Actinobacteria cells experienced physiological stresses at 25 °C, showing the upregulation of chaperone proteins, GroEL and catalase, KatE. Level of proteins involved in the assembly of 50S ribosomal proteins and L29 in 50S ribosomal proteins increased at 13 °C, which suggested distinct roles of many ribosomal proteins under different conditions. Taken together, our data highlights the cellular filamentation and protein homeostasis of a psychrophilic YJ56 strain in coping with high-temperature stress.

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“…Protein acetylation is one of the major post-translational modifications (PTMs) which play an important role in cell signaling and occurs when the cell encounters specific environmental stress conditions [ 68 – 70 ]. RimI encoded by rimI is a ribosomal protein S18 acetyltransferase which catalyzes the acetylation of the N-terminal alanine of ribosomal protein S18; and also acts as an N-epsilon-lysine acetyltransferase that catalyzes acetylation of several proteins [ 71 , 72 ]. In this study, an insertion mutation (S33_S34insC) in rimI was found in all S .…”

Section: Discussionmentioning

confidence: 99%

Genomic alterations involved in fluoroquinolone resistance development in Staphylococcus aureus

et al. 2023

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Aim Fluoroquinolone (FQ) is a potent antibiotic class. However, resistance to this class emerges quickly which hinders its application. In this study, mechanisms leading to the emergence of multidrug-resistant (MDR) Staphylococcus aureus (S. aureus) strains under FQ exposure were investigated. Methodology S. aureus ATCC 29213 was serially exposed to ciprofloxacin (CIP), ofloxacin (OFL), or levofloxacin (LEV) at sub-minimum inhibitory concentrations (sub-MICs) for 12 days to obtain S. aureus -1 strains and antibiotic-free cultured for another 10 days to obtain S. aureus-2 strains. The whole genome (WGS) and target sequencing were applied to analyze genomic alterations; and RT-qPCR was used to access the expressions of efflux-related genes, alternative sigma factors, and genes involved in FQ resistance. Results A strong and irreversible increase of MICs was observed in all applied FQs (32 to 128 times) in all S. aureus-1 and remained 16 to 32 times in all S. aureus-2. WGS indicated 10 noticeable mutations occurring in all FQ-exposed S. aureus including 2 insdel mutations in SACOL0573 and rimI; a synonymous mutation in hslO; and 7 missense mutations located in an untranslated region. GrlA, was found mutated (R570H) in all S. aureus-1 and -2. Genes encoding for efflux pumps and their regulator (norA, norB, norC, and mgrA); alternative sigma factors (sigB and sigS); acetyltransferase (rimI); methicillin resistance (fmtB); and hypothetical protein BJI72_0645 were overexpressed in FQ-exposed strains. Conclusion The emergence of MDR S. aureus was associated with the mutations in the FQ-target sequences and the overexpression of efflux pump systems and their regulators.

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Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu

Cited by 14 publications

References 35 publications

Escherichia coli RimI Encodes Serotonin N-Acetyltransferase Activity and Its Overexpression Leads to Enhanced Growth and Melatonin Biosynthesis

Escherichia coli RimI Encodes Serotonin N-Acetyltransferase Activity and Its Overexpression Leads to Enhanced Growth and Melatonin Biosynthesis

Morphological and physiological adaptations of psychrophilic Pseudarthrobacter psychrotolerans YJ56 under temperature stress

Genomic alterations involved in fluoroquinolone resistance development in Staphylococcus aureus

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