1975
DOI: 10.1016/s0022-2836(75)80177-x
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Ribosomal proteins S5, S11, S13 and S19 localized by ekectron microscopy of antibody-labeled subunits

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Cited by 112 publications
(38 citation statements)
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“…Sedimentation equilibrium experiments gave molecular weight values that agreed with those obtained from the amino acid sequence [20,21] , namely 12 257 for S8 and 18 839 for L6. Figure 1 shows sedimentation equilibrium experiments with the four proteins.…”
Section: Resultssupporting
confidence: 72%
See 1 more Smart Citation
“…Sedimentation equilibrium experiments gave molecular weight values that agreed with those obtained from the amino acid sequence [20,21] , namely 12 257 for S8 and 18 839 for L6. Figure 1 shows sedimentation equilibrium experiments with the four proteins.…”
Section: Resultssupporting
confidence: 72%
“…Protein concentrations were determined by three methods: (1) A fluorescamine assay using lysozyme as a standard ]18]-(2) Hydrolysis of aliquots of the protein solution with 6 N hydrochloric acid containing 0.2% 2-mercaptoethanol under nitrogen at 1 10°C for 20 h, followed by amino acid analysis on a Durrum D-500 analyzer. (3) A nitrogen assay [ 191 using ammonium sulfate as a standard and calculation of the nitrogen content of each protein from its amino acid sequence [20,21] . …”
mentioning
confidence: 99%
“…Those sites functioning in translation are clustered into part of the ribosome, composing approximately two-thirds of its volume, that we have named the "translational domain. " Functional sites contained in the translational domain include: the initiation factor binding sites (1)(2)(3)(4) located in the cleft of the small subunit, the messenger binding sites located on the platform of the small subunit (3,(5)(6)(7), the peptidyltransferase and the 5S RNA located on the central protuberance of the large subunit (8)(9)(10)(11), and proteins mediating the GTP-dependent steps of translation that are found on the L7/L12 stalk of the large subunit (12). Together, these sites define the translational domain.…”
mentioning
confidence: 99%
“…Electron microscopy studies of Lake et al [26], using antibody markers, show that S 13 and S 19 are located side by side on the head of the 30 S (3) (4) (5) subunit, although they were found to be more separated in a similar study by Tischendorf et al…”
Section: Discussionmentioning
confidence: 86%