Ribosomal RNA and ribosomal proteins in corynebacteria

Martı́n, Juan F.; Barreiro, Carlos; González-Lavado, Eva; Barriuso, Mónica

doi:10.1016/s0168-1656(03)00160-3

Cited by 41 publications

(30 citation statements)

References 91 publications

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“…Annotation data were used to compare the gene organization among the three mycoplasmas and to the E. coli and Corynebacterium glutamicum models (Martín et al, 2003). The translated amino acid sequences of L33 ribosomal and gat (A, B and C) proteins were retrieved from the database and used to search for related sequences by using BLAST tools (Altschul et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

“…In the three sequenced genomes, we found 10 ribosomal protein gene clusters encoding 42 ribosomal proteins in M. synoviae, and 8 clusters encoding 39 ribosomal proteins in both M. hyopneumoniae strains, as shown in Figure 1. In the E. coli and C. glutamicum models (Martín et al, 2003), there are 11 gene clusters encoding 41 and 42 ribosomal proteins, respectively. In E. coli, the S10 operon encodes 11 ribosomal proteins in the following order: S10, L3, L2, L4, L23, S19, L22, S3, L16, L29 and S17, and is located close to the spc operon that includes 12 genes which encode the proteins L14, L24, L5, S14, S8, L6, L18, S5, L30, L15, Sec Y and L36 (Cerretti et al, 1983).…”

Section: Organization Of Gene Clusters Encoding Ribosomal Proteinsmentioning

confidence: 99%

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Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae

et al. 2007

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This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide release factor (prfA) were present in the genomes of M. hyopneumoniae and M. synoviae. Nineteen aminoacyl-tRNA synthases had been previously identified in both mycoplasmas. In the two strains of M. hyopneumoniae, J and 7448, only one set of 5S, 16S and 23S rRNAs had been identified. Two sets of 16S and 23S rRNA genes and three sets of 5S rRNA genes had been identified in the M. synoviae genome.

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Section: Methodsmentioning

confidence: 99%

Section: Organization Of Gene Clusters Encoding Ribosomal Proteinsmentioning

confidence: 99%

Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae

et al. 2007

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“…BL1655 to BL1657 and BL1293 to BL1296 share similarities with MetNPQ, a high-affinity ABC transporter of B. subtilis (53,35, and 36% identities) (48) and a cystine ABC transporter of E. coli (48,31,33, and 26% identities), respectively. Other putative cystine or methionine transporters are present in the B. aurantiacum genome.…”

Section: Comparison Of the Expression Profiles After The Growth Of Stmentioning

confidence: 99%

“…A systematic search for E. coli, B. subtilis, C. glutamicum, and M. tuberculosis genes involved in sulfur metabolism was performed. The high-GC-content Gram-positive bacteria C. glutamicum and M. tuberculosis are phylogenetically related to Brevibacterium strains (33,49). The genes were renamed according to B. subtilis and C. glutamicum orthologues.…”

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confidence: 99%

Global Regulation of the Response to Sulfur Availability in the Cheese-Related Bacterium Brevibacterium aurantiacum

Forquin

Hébert

Roux

et al. 2011

Appl Environ Microbiol

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In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway, thiolation pathways that produce cysteine (cysE and cysK) or homocysteine (metX and metY) from sulfide, at least one gene of the transsulfuration pathway (aecD), and genes encoding three MetE-type methionine synthases. We also compared the expression profiles of B. aurantiacum ATCC 9175 during sulfur starvation or in the presence of sulfate. Under sulfur starvation, 690 genes, including 21 genes involved in sulfur metabolism and 29 genes encoding amino acids and peptide transporters, were differentially expressed. We also investigated changes in pools of sulfur-containing metabolites and in expression profiles after growth in the presence of sulfate, cystine, or methionine plus cystine. The expression of genes involved in sulfate assimilation and cysteine synthesis was repressed in the presence of cystine, whereas the expression of metX, metY, metE1, metE2, and BL613, encoding a probable cystathionine-␥-synthase, decreased in the presence of methionine. We identified three ABC transporters: two operons encoding transporters were transcribed more strongly during cysteine limitation, and one was transcribed more strongly during methionine depletion. Finally, the expression of genes encoding a methionine ␥-lyase (BL929) and a methionine transporter (metPS) was induced in the presence of methionine in conjunction with a significant increase in volatile sulfur compound production.

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“…Sometimes tRNA genes are found between rrs and rrl or downstream of rrf genes. However, other configurations of the rrn operon with respect to the classical one (rrs-rrl-rrf) are also seen (1). The rRNA genes are cotranscribed as a polycistronic precursor, which undergoes posttranscriptional modifications.…”

mentioning

confidence: 99%

High Prevalence of Diverse Insertion Sequences within the rRNA Operons of Mycoplasma bovis

Amram

Borovok

Nachum‐Biala

et al. 2016

Appl Environ Microbiol

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T he bacterial 70S ribosome is composed of large (50S) and small (30S) subunits, each of which is a ribonucleoprotein complex. The ribosome contains three rRNA genes: genes encoding two large-subunit-associated rRNAs (23S and 5S) and the small-subunit rRNA (16S). The genes encoding rRNA usually form an operon designated rrn with a typical configuration of rrs-rrl-rrf (where rrs, rrl, and rrf are the genes encoding the 16S, 23S, and 5S rRNAs, respectively). Sometimes tRNA genes are found between rrs and rrl or downstream of rrf genes. However, other configurations of the rrn operon with respect to the classical one (rrs-rrl-rrf) are also seen (1). The rRNA genes are cotranscribed as a polycistronic precursor, which undergoes posttranscriptional modifications. Sometimes, transcription of rRNA genes may be carried out from more than one promoter, as has been shown for Escherichia coli, in which transcription of rRNA genes occurs from two tandem promoters, P1 and P2, separated by about 100 bp (2).The number of rrn operons varies among bacterial species. For example Mycobacterium tuberculosis (44) and Rickettsia prowazekii (3) have one rrn operon, and Helicobacter pylori has two rrn operons (4), while E. coli, Bacillus subtilis, and Clostridium paradoxum contain 7, 10, and 15 rrn operons per genome, respectively (5-7). In the small, cell-wall-less Mycoplasma species, belonging to the class Mollicutes, the number of rrn operons usually varies from one to two, although some species (e.g., Mycoplasma canis and Mycoplasma cynos) have three rrn loci (deduced from accession no. CP011368.1 and HF559394.1).A systematic analysis of the complete genome of the bovine pathogen Mycoplasma bovis type strain PG45 (used as a reference strain in many studies) revealed the presence of two tandemly positioned rrn loci (called hereinafter rrn1 and rrn2, correcting their annotations as rrn3 and rrn4 [GenBank CP002188]). Each of the two rrn genes consists of the two alleles rrs and rrl. In the M. bovis PG45 genome annotation, the genes MBOPG45_0956 and MBOPG45_0957 correspond to rrs1 and rrl1, respectively, and MBOPG45_0958 and MBOPG45_0959 to rrs2 and rrl2, respectively (8). The rrn genes are separated by five intergenic regions (IGRs): IGR-1 (located upstream of rrs1), IGR-2 (between rrs1 and rrl1), IGR-3 and IGR-4 (separating rrl1 and rrs2 and rrs2

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Ribosomal RNA and ribosomal proteins in corynebacteria

Cited by 41 publications

References 91 publications

Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae

Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae

Global Regulation of the Response to Sulfur Availability in the Cheese-Related Bacterium Brevibacterium aurantiacum

High Prevalence of Diverse Insertion Sequences within the rRNA Operons of Mycoplasma bovis

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