Ribosomal rna in avian leukosis virus particles

Obara, Tadashi; Bolognesi, Dani P.; Bauer, Heinz

doi:10.1002/ijc.2910070320

Cited by 47 publications

(20 citation statements)

References 35 publications

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“…Similar observations were noted in studies of the avian RNA tumour virus tRNA (Obara et aL, 1971). When [3H]uridine was added to cells at the time of infection, only 62S genomic RNA was labelled in virus particles.…”

Section: Discussionsupporting

confidence: 82%

“…However, when the RNA was examined by absorbance analysis, 4S RNA was present in equal proportion to 62S RNA. Obara et al (1971) have suggested that the interval between labelling and incorporation into the particle for a given RNA molecule is much shorter for genomic RNA. Transfer RNA must first be synthesized in the nucleus and then transported to the cytoplasm, a process that would increase the interval between uptake of label and incorporation into virus particles.…”

Section: Discussionmentioning

confidence: 99%

“…Small RNA molecules ranging in size from 4S to 7S are associated with various virus particles (Bonar et al, 1967;Carnegie et al, 1969;Erikson, 1969;Kolakofsky, 1972;Obara et al, 1971;Sawyer & Dahlberg, 1973). Some of these RNAs function during virus replication (Dahlberg et al, 1974) and are synthesized by the host cell and packaged by the virus during assembly (Carnegie et al, 1969;Erikson & Erikson, 1971;Kolakofsky, 1972;Sawyer & Dahlberg, 1973).…”

Section: Introductionmentioning

confidence: 99%

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Transfer RNAs Associated with Vesicular Stomatitis Virus

Isaac

Keene

1981

Journal of General Virology

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SUMMARYThe predominant RNAs in purified VSV particles are 425 and 4S in size. The 4S RNA is host transfer RNA that did not incorporate detectable radiolabel during VSV infection and was detected by in vitro labelling. Surprisingly, when BHK cells were prelabelled for 30 to 54 h before infection, the incorporation of [3H]uridine and [a2p]orthophosphate into virus tRNAs remained very low and virus tRNAs were found to have a 5-to 15-fold lower specific activity than the total host tRNA, the value depending, in part, upon the period of prelabelling. Two-dimensional gel electrophoresis and partial sequence analysis indicated that the virus tRNAs include most species of host tRNA and no singly predominant species was apparent. Transfer RNAs are packaged by several enveloped viruses, but we have not found 4S RNA in reovirus, which lacks an envelope. We suggest that VSV contains a membrane-associated population of tRNA which has a slower rate of turnover than the total population of cellular tRNA.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transfer RNAs Associated with Vesicular Stomatitis Virus

Isaac

Keene

1981

Journal of General Virology

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“…HSRV virions were clarified from cell supernatants by ultracentrifugation on days 2-5 after infection when the cytopathic effect was almost complete. Virus was resuspended in TKM buffer (Obara et al, 1971) and stored at -20 o C until further processing.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of the genome of human spumaretrovirus

Rethwilm

Darai²,

Rösen³

et al. 1987

Gene

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SUMMARYDNA ofhuman spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage A. and bacterial plasmid vectors. The recombinant plasm.ids harboring viral DNA were characterized by Southem blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained from viral DNA. The recombinant clones isolated contained viral DNA inserts which rangein size from 2.2 kb to 15.4 kb. The recombinant clones allowed to construct a physical map of the complete HSRV provirus of 12.2 kb.

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“…In addition to the RNA representing the viral genome, other RNAs of lesser weight are known to be associated with the virions and can be isolated by standard procedures from the viral preparations. These RNAs are mainly represented by two peaks, about 18 and 28 S, and it has been suggested that they are, at least in part, of cellular origin, possibly incorporated as a ribosomal component into the virions during their maturation by budding at the cellular membranes (6,12,13).The purpose of this study is 2-fold. First, we have undertaken a statistical analysis of all molecules present in a given preparation of heat-treated high-molecular-weight viral RNA to help resolve the question of whether the appearance of the large number of subunits of intermediate size is due to random cleavage or to a specific process resulting in a number of smaller molecules of well-defined size.…”

mentioning

confidence: 95%

Analysis of oncornavirus RNA subunits by electron microscopy.

Heine¹,

Weber²,

Cottler‐Fox³

et al. 1975

Proc. Natl. Acad. Sci. U.S.A.

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Subunits of oncornavirus (avian myeloblastosis virus) RNA were isolated from purified 60-70S viral RNA by heat dissociation. Molecules sedimenting at 35 S, assumed to be the major component of the viral genotne, were visualized in the electron microscope and their lengths were statistically analyzed. The results indicate a rather heterogeneous population of molecules with five distinct, reproducible size groups, an observation that excludes the assumption of random degradation of the genome.In addition, molecules of 28 and iSS RNA, always present in oncornavirus RNA preparations, were examined with the same method. Some of these molecules possess secondarystructure regions similar to those characteristic for ribosomal RNA.Most of the oncornaviruses have a genome of single-stranded RNA which sediments between 60 and 70 S (1, 2). This RNA can be dissociated by heat or by dimethylsulfoxide into subunits sedimenting at lower speed. The complexity of the high-molecular-weight genome is reflected in the appearance, after dissociation, of a number of subunits each having a distinct sedimentation coefficient. Low-molecular-weight virus-associated RNAs sediment between 4 and 8 S; the high-molecular-weight viral subunits sediment around 35 S and are described as corresponding in size to molecular weights of about 3 X 106 (3-6). Moreover, a wide range of molecules of intermediate sizes can usually be detected after complete disruption of hydrogen bonds linking together the genome (7-11). The latter molecules are thought to be degradation products due either to shearing during preparation of the nucleic acids or in the virions per se. In addition to the RNA representing the viral genome, other RNAs of lesser weight are known to be associated with the virions and can be isolated by standard procedures from the viral preparations. These RNAs are mainly represented by two peaks, about 18 and 28 S, and it has been suggested that they are, at least in part, of cellular origin, possibly incorporated as a ribosomal component into the virions during their maturation by budding at the cellular membranes (6,12,13).The purpose of this study is 2-fold. First, we have undertaken a statistical analysis of all molecules present in a given preparation of heat-treated high-molecular-weight viral RNA to help resolve the question of whether the appearance of the large number of subunits of intermediate size is due to random cleavage or to a specific process resulting in a number of smaller molecules of well-defined size.Second, we are presenting evidence that a certain number of virus-associated RNA molecules, especially those found in the 28S region, are similar in appearance to cellular ribosomal RNA as characterized by secondary structures visible within each molecule when studied in the electron microscope. In both viral and cellular preparations, these molecules are of similar configuration.MATERIALS AND METHODS Virus. Avian myeloblastosis virus BAI strain A (AMV) was obtained from Dr. J. W. Beard, Life Sciences, Inc., St. Peters...

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Ribosomal rna in avian leukosis virus particles

Cited by 47 publications

References 35 publications

Transfer RNAs Associated with Vesicular Stomatitis Virus

Transfer RNAs Associated with Vesicular Stomatitis Virus

Molecular cloning of the genome of human spumaretrovirus

Analysis of oncornavirus RNA subunits by electron microscopy.

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