1998
DOI: 10.1080/10409239891204189
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Ribosomal tRNA Binding Sites: Three-Site Models of Translation

Abstract: The first models of translation described protein synthesis in terms of two operationally defined tRNA binding sites, the P-site for the donor substrate, the peptidyl-tRNA, and the A-site for the acceptor substrates, the aminoacyl-tRNAs. The discovery and analysis of the third tRNA binding site, the E-site specific for deacylated tRNAs, resulted in the allosteric three-site model, the two major features of which are (1) the reciprocal relationship of A-site and E-site occupation, and (2) simultaneous codon-ant… Show more

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Cited by 50 publications
(27 citation statements)
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“…This is not surprising, because it is well-known that only the "top half " of a tRNA molecule containing the acceptor stem and the T⌿C stem-loop, which exist in sRNA-85, are essential for recognition by the CCAadding enzyme (43). Note that CCA is also important for binding uncharged tRNAs to the E site and for the translocation of tRNA from the P to the E site (44). Like a bona fide tRNA, the data, presented in Fig.…”
Section: Figmentioning
confidence: 91%
“…This is not surprising, because it is well-known that only the "top half " of a tRNA molecule containing the acceptor stem and the T⌿C stem-loop, which exist in sRNA-85, are essential for recognition by the CCAadding enzyme (43). Note that CCA is also important for binding uncharged tRNAs to the E site and for the translocation of tRNA from the P to the E site (44). Like a bona fide tRNA, the data, presented in Fig.…”
Section: Figmentioning
confidence: 91%
“…The eubacterial proteins, EFTu, EFTs, and EFG (eEF1␣, eEF1␤␥, and eEF2 of eukaryotes), facilitate these processes on ribosomes. Reviews are available (22,36,116,144,146,202).…”
Section: Protein Chain Elongationmentioning
confidence: 99%
“…Indeed, the protection analysis demonstrates that EFP is in contact with the A site of domain V of the 23S rRNA. In binding directly to the A site, EFP could directly increase the affinity of the incoming aminoacyl-tRNA (22,181). The binding of EFP to the A site also enhances the reactivity of bases in the 50S E site (Aoki et al, submitted).…”
Section: Possible Role Of Efp In the Peptidyl Transferase Reactionmentioning
confidence: 99%
“…First, the chemical protections of the large subunit by the acceptor ends of the tRNAs seemed to depend on (and thus potentially report on) the three terminal nucleotides of the molecule (the CCA-end; Burkhardt et al 1998). Indeed, examination of the crystallographic data indicates that the A and P site footprints spatially overlap and only some of the specific protections seem to represent direct tRNA contacts.…”
Section: Introductionmentioning
confidence: 99%