Ribosome Metabolism in Excised Slices of Jerusalem Artichoke Tuber

Setterfield, G.; Sparkuhl, Joachim; Byrne, Henry

doi:10.1515/9783111671062-019

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthesis of the presumed rRNA in the R-ZP cells may lead to its incorporation into ribosomes and subsequently polysomes. This sequence of events occurs in slices of artichoke tuber and the polysomes are capable of in vitro protein synthesis (17). Webster and Van't Hof (21) observed that RNA and subsequent protein synthesis are prerequisites for DNA synthesis and mitosis in pea root meristems in the G, and G2 stages, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…They reported that synthesis of mRNA starts between 0 and 2 hr after excision, while synthesis of rRNA starts between 2 and 12 hr. Using Setterfield's wound-healing study (17) as a basis, we suggest that the [3H]UR in the nucleoli in 2-hr cells is in mRNA and that most of the [3H]UR observed in the cytoplasm of the R-ZP cells from 8 to 26 hr was probably rRNA. Uridine incorporation may be related to subsequent DNA syn thesis in the R-ZP cells.…”

Section: Discussionmentioning

confidence: 99%

“…2C, 2D, and 2E). Setterfield et al (17) observed that RNA synthesis occurs as early as 10 min in cells excised from Jeru salem artichoke tubers. They reported that synthesis of mRNA starts between 0 and 2 hr after excision, while synthesis of rRNA starts between 2 and 12 hr.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Incorporation of Tritiated Thymidine and Uridine into Adventitious-root Initial Cells of Vigna radiata

Tripepi

Heuser

Shannon

1983

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Adventitious roots in mung bean [Vigna radiata (L.) R. Wilcz. cv. Berken] cuttings develop from specific “rooting-zone parenchyma” (R-ZP) cells. Microautoradiography was used to determine the timing of thymidine and uridine incorporation into the R-ZP cells, prior to the first cell division, in the presence or absence of naphthaleneacetic acid (NAA). Since 6-3H-thymidine incorporation reached a maximum between 11 and 14 hours, we suggest that the R-ZP cells were in the G1 phase when the cuttings were taken. Tritiated uridine was incorporated into the RNA of the R-ZP cells 2 hours after the cuttings were placed in the labeled solution. DNA synthesis and cell division of the R-ZP cells occurred along the entire length of the hypocotyl (basal, middle, and top segments), but these initial events were not sufficient to result in the subsequent formation of adventitious roots. NAA promoted adventitious root formation in the cuttings but it had no apparent effect on nucleic acid labeling nor initial cell division of the R-ZP cells. The initial division of the R-ZP cells appears to be a wounding response and occurs in the presence or absence of exogenous auxin.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%