Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Chetoui, Hafid; Delhalle, E; Osterrieth, Paul M.; Rousseaux, D

doi:10.1128/jcm.33.10.2637-2642.1995

Cited by 11 publications

(4 citation statements)

References 30 publications

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“…Ribotyping has been used for the study of many bacterial species responsible for nosocomial infections (6,11) and also for different species of the genus Salmonella (12,19,30,35). In our study, ribotyping revealed an identical pattern ( Fig.…”

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confidence: 60%

Characterization of Extended-Spectrum β-Lactamase-Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods

et al. 1999

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During 1994, 10 isolates of extended-spectrum β-lactamase-producing Salmonella typhimurium were recovered from children transferred to our hospital from two different centers. Two additional isolates were recovered from two nurses from one of these centers. The aim of this study was to determine if there is any relationship between these isolates. The characterization was done by phenotypic and genotypic methods: biotyping, phage typing, antibiotic susceptibility pattern determination, plasmid analysis, ribotyping (by the four endonucleases EcoRI,SmaI, BglII, and PvuII), pulsed-field gel electrophoresis (PFGE) of genome macrorestriction patterns with XbaI, and randomly amplified polymorphic DNA (RAPD) pattern determination (with the three primers 217 d2, B1, and A3). The same biotype, the same serotype, and an identical antibiotype were found. All isolates were resistant to oxyimino-β-lactams, gentamicin, tobramycin, and sulfamethoxazole-trimethoprim. All isolates showed an indistinguishable pattern by ribotyping and very similar patterns by PFGE and RAPD. The overall results indicated the spread of a closely related strain of S. typhimurium in children and nurses.

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confidence: 60%

Characterization of Extended-Spectrum β-Lactamase-Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods

et al. 1999

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“…(iii) Ribotyping. Ribotyping with EcoRI and HindIII with a nonradioactive probing system (acetylaminofluorene rRNA Kit I from Eurogentec, Seraing, Belgium) was carried out as reported earlier (1).…”

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confidence: 99%

Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia

et al. 1997

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Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection. Vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. No further cases were diagnosed once the use of this saline was discontinued. Twenty-six isolates, including 9 outbreak-related strains from case patients and contaminated saline as well as 17 control strains, were tested comparatively by biotyping, ribotyping with EcoRI and HindIII, and pulsed-field gel electrophoresis (PFGE) with SpeI. Macrorestriction analysis revealed nine PFGE groups and was more discriminating than ribotyping (seven ribotypes) and biotyping (two biovars). Among the outbreak-related isolates, one B. pickettii type was found by the three typing methods. Furthermore, PFGE was useful for subdividing ribotypes and for distinguishing isolates involved in the outbreak from all epidemiologically unrelated strains. Burkholderia pickettii is a nonfermentative gram-negative rod isolated from environmental sources and infrequently from clinical samples (5). It is an occasional agent associated with pseudobacteremias or asymptomatic colonization of patients (8, 13, 19). However, this bacterium has been identified in a few reports as the etiologic agent of true bacteremias (2, 4, 7, 8, 12, 14, 15). Most of the bloodstream infections were traced to the contamination of parenteral fluids or of medical equipment (14). We report here on an outbreak of B. pickettii bacteremias at a 650-bed teaching hospital in Liege, Belgium. From June to August 1994, six immunocompromised patients developed B. pickettii bacteremia and/or catheter infection. All had permanent central venous catheters. The patency of these lines was maintained by regular flushing with heparin solution. The organism was isolated from multiple vials containing commercially available, "sterile" saline without preservative (Physio-Sterop; Sterop, Brussels, Belgium) used for dilution of heparin. Randomly selected isolates originating from both clinical cultures and contaminated vials, as well as control strains, were characterized by biotyping, ribotyping, and DNA typing by pulsed-field gel electrophoresis (PFGE).

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“…Ribotyping was performed with the restriction enzyme PvuII by using a [␣-32 P]dCTP-labeled gene probe from Escherichia coli 16S-plus-23S RNA. Strains that differed by more than two bands were considered to be different clones, while single band differences were interpreted as subtypes (2).…”

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confidence: 99%

Molecular Characterization of Multidrug-Resistant Streptococcus pneumoniae Isolates in Korea

et al. 2000

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Pulsed-field gel electrophoresis, ribotyping, and fingerprinting analysis of 22 invasive isolates of multidrug-resistant (MDR) pneumococci from Korea showed that 59 to 82% were genetically related. DNA sequencing of the PBP 2B gene showed relatively uniform alterations in nucleotides (5.4 to 7.8%) and amino acids (3.0 to 4.3%), while Asn-276→Lys, Arg-285→Cys and Ser-305→Phe substitutions were unique to Korean MDR strains, suggesting the spread of a few epidemic clones of resistant pneumococci within Korea.

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Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Cited by 11 publications

References 30 publications

Characterization of Extended-Spectrum β-Lactamase-Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods

Characterization of Extended-Spectrum β-Lactamase-Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods

Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia

Molecular Characterization of Multidrug-Resistant Streptococcus pneumoniae Isolates in Korea

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