Ribotyping of lactobacilli isolated from spoiled beer

Abstract

Twenty-nine Lactobacillus strains contaminating beers in different Czech breweries as well as representative type strains obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and a probe made complementary to 16S and 23S rRNA genes. Biochemical test results assigned the 29 strains to the species L. brevis, L. plantarum, L. buchneri and L. paracasei subsp. paracasei. Ribotyping separated L. brevis, L. plantarum and L. paracasei subsp. paracasei strains into species-specific ribogroups in full correspondence with biotyping; L. buchneri strains were split into two ribogroups. Characteristic band patterns for each species and even typical bands of certain sizes were observed.

Development of a multilocus sequence typing method for analysis of Lactobacillus plantarum strains

“…Yansanjav et al (2003) used ribotyping in a study of five L. plantarum strains. These five strains formed two ribotype patterns and the authors found that strains isolated from different breweries shared the same ribotype.…”

“…Genotypic methods used for L. plantarum strain typing are typically PCR-based methods, for example randomly amplified polymorphic DNA (Johansson et al, 1995;Oneca et al, 2003;Elegado et al, 2004) or variations of restriction enzyme analysis, for example ribotyping (Yansanjav et al, 2003;Rodas et al, 2005) and pulsed-field gel electrophoresis (Sánchez et al, 2004). Multilocus sequence typing (MLST) has recently been shown to be a powerful technique for bacterial typing.…”

Detection of resistance of lactic acid bacteria to a mixture of the hop analogue compounds tetrahydroiso-alpha-acids by noninvasive measurement of intracellular pH

“…The 19 LAB beer isolates included in this study were selected from the culture collection at the Research Institute of Brewing and Malting, Prague, Czech Republic, and previously characterized by carbohydrate fermentation, plasmid and ribotyping patterns. Based on these criteria all of the LAB beer strains included in this study belonged to different clonal lines (Yansanjav et al. 2003 and Yansanjav, unpublished data).…”

Application of molecular identification tools for Lactobacillus, with a focus on discrimination between closely related species: A review

“…Ribotyping affords good subspecies differentiation and has successfully been used for both species and strain discrimination within Lactobacillus (Kitahara, Sakata, & Benno, 2005;McCartney, Wang, & Tannock, 1996). It has successfully been applied to detect individual species or strains within the L. acidophilus complex, L. casei, L. delbrueckii, L. fermentum, L. helveticus, L. plantarum, L. reuteri, L. rhamnosus, L. sakei, L. crispatus and L. gasseri species (Charteris et al, 1997;Giraffa & Neviani, 2000;Holzapfel et al, 2001;Ryu et al, 2001;Yansanjav, Svec, Sedlacek, Hollerova, & Nemec, 2003). The strongholds of ribotyping are: it allows typing of all isolates, it is reproducible, it has a high discriminating power, and it generates simple patterns.…”