Ribozyme-Mediated Decrease in Mumps Virus Nucleocapsid mRNA Level and Progeny in Infected Vero Cells

Albuquerque-Silva, João; Milican, Fabienne; Bollen, Alex; Houard, Sophie

doi:10.1089/oli.1.1999.9.279

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…The selective destruction of the RNAs of mumps virus [172], influenza virus [173], alphavirus [174] and human papillomavirus type 16 [175] has also been accomplished with these small ribozymes.…”

Section: Ribozyme‐based Therapeuticsmentioning

confidence: 99%

Ribozymes: recent advances in the development of RNA tools

et al. 2003

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The discovery 20 years ago that some RNA molecules, called ribozymes, are able to catalyze chemical reactions was a breakthrough in biology. Over the last two decades numerous natural RNA motifs endowed with catalytic activity have been described. They all fit within a few well-defined types that respond to a specific RNA structure. The prototype catalytic domain of each one has been engineered to generate trans-acting ribozymes that catalyze the site-specific cleavage of other RNA molecules. On the 20th anniversary of ribozyme discovery we briefly summarize the main features of the different natural catalytic RNAs. We also describe progress towards developing strategies to ensure an efficient ribozyme-based technology, dedicating special attention to the ones aimed to achieve a new generation of therapeutic agents.

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Section: Ribozyme‐based Therapeuticsmentioning

confidence: 99%

Ribozymes: recent advances in the development of RNA tools

et al. 2003

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“…Hairpin and hammerhead cleavage has also been used in order to cope with infections by other viruses such as influenza virus [95], lymphocytic choriomeningitis virus (LCMV) [96], mumps virus [97], alphavirus [98], and bovine leukaemia virus [99].…”

Section: Other Virusesmentioning

confidence: 99%

RNA-Mediated Therapeutics: From Gene Inactivation to Clinical Application

Kalavrizioti¹,

Vourekas²,

Stamatopoulou³

et al. 2006

CTMC

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The specific targeting and inactivation of gene expression represents nowadays the goal of the mainstream basic and applied biomedical research. Both researchers and pharmaceutical companies, taking advantage of the vast amount of genomic data, have been focusing on effective endogenous mechanisms of the cell that can be used against abnormal gene expression. In this context, RNA represents a key molecule that serves both as tool and target for deploying molecular strategies based on the suppression of genes of interest. The main RNA-mediated therapeutic methodologies, deriving from studies on catalytic activity of ribozymes, blockage of mRNA translation and the recently identified RNA interference, will be discussed in an effort to understand the utilities of RNA as a central molecule during gene expression.

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“…There is also indication from reverse transcriptase‐polymerase chain reaction (RT‐PCR) and reverse ligation (RL)‐PCR protocols of messenger RNA cleavage at the targeted position in cellular RNA extracts [61, 62]. Recently, more potent ribozyme‐mediated effect on viral and cancer cell growth compared to noncatalytic RNAs was reported [63, 64]. However, in some instances, hammerhead ribozymes have not proven to be more effective than AS‐ON and instead give equal degrees of gene suppression.…”

Section: Anti‐mrna Strategiesmentioning

confidence: 99%

Suppression of Gene Expression by Targeted Disruption of Messenger RNA: Available Options and Current Strategies

Jen

Gewirtz

2000

STEM CELLS

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At least three different approaches may be used for gene targeting including: A) gene knockout by homologous recombination; B) employment of synthetic oligonucleotides capable of hybridizing with DNA or RNA, and C) use of polyamides and other natural DNA-bonding molecules called lexitropsins.Targeting mRNA is attractive because mRNA is more accessible than the corresponding gene. Three basic strategies have emerged for this purpose, the most familiar being to introduce antisense nucleic acids into a cell in the hopes that they will form Watson-Crick base pairs with the targeted gene's mRNA. Duplexed mRNA cannot be translated, and almost certainly initiates processes which lead to its destruction. The antisense nucleic acid can take the form of RNA expressed from a vector which has been transfected into the cell, or take the form of a DNA or RNA oligonucleotide which can be introduced into cells through a variety of means. DNA and RNA oligonucleotides can be modified for stability as well as engineered to contain inherent cleaving activity.It has also been proven that because RNA and DNA are very similar chemical compounds, DNA molecules with enzymatic activity could also be developed. This assumption proved correct and led to the development of a "general-purpose" RNA-cleaving DNA enzyme. The attraction of DNAzymes over ribozymes is that they are very inexpensive to make and that because they are composed of DNA and not RNA, they are inherently more stable than ribozymes.Although mRNA targeting is impeccable in theory, many additional considerations must be taken into account in applying these strategies in living cells including mRNA site selection, drug delivery and intracellular localization of the antisense agent. Nevertheless, the ongoing revolution in cell and molecular biology, combined with advances in the emerging disciplines of genomics and informatics, has made the concept of nontoxic, cancer-specific therapies more viable then ever and continues to drive interest in this field.

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Ribozyme-Mediated Decrease in Mumps Virus Nucleocapsid mRNA Level and Progeny in Infected Vero Cells

Cited by 8 publications

References 27 publications

Ribozymes: recent advances in the development of RNA tools

Ribozymes: recent advances in the development of RNA tools

RNA-Mediated Therapeutics: From Gene Inactivation to Clinical Application

Suppression of Gene Expression by Targeted Disruption of Messenger RNA: Available Options and Current Strategies

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