Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli

Matsumoto, Ken’ichiro; Saito, Juri; Yokoo, Toshinori; Hori, Chiaki; Nagata, Akihiro; Kudoh, Yuki; Ooi, Toshihiko; Taguchi, Seiichi

doi:10.1016/j.jbiosc.2019.03.002

Cited by 13 publications

(4 citation statements)

References 19 publications

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“…To assess the role of the RuBisCO-based pathway for GL-based PHA synthesis, E. coli JW2946 with the deleted glycolate oxidase gene (DglcD) was used, and this engineered strain accumulated poly(GL-co-3HB)s with GL fractions of 7.8−15.1 mol %. 64 Malic Acid (MA). Malic acid (MA) is a C4-dicarboxylated acid that can be commonly used as an aromatic flavor enhancer in the food and pharmaceutical industry.…”

Section: Chemicals With the Cbb Cycle Equipped Strainsmentioning

confidence: 99%

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Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle

Feng

Gao

et al. 2023

ACS Sustainable Chem. Eng.

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Global climate change caused by greenhouse gas emission is the fundamental challenge facing mankind. Biological carbon dioxide fixation has recently attracted much attention. The Calvin–Benson–Bassham (CBB) cycle is a major carbon fixation pathway in the biosphere, and it is the most intensively studied because of its prominence and key role in nature as the primary pathway for net CO2 fixation. It was recently shown that expressing the enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and phosphoribulokinase (PRK) in Escherichia coli, Saccharomyces cerevisiae, Methylobacterium extorquens, Kluyveromyces marxianus, and Pichia pastoris could achieve CO2 fixation for the production of value-added chemicals. In addition, expressing the synthetic CBB cycle allowed strains to grow autotrophically on CO2. Here, we review the current understanding of the CBB cycle for CO2 fixation, summarize research progress in the assembly of the CBB cycle in heterotrophic microorganisms or developing synthetic autotrophs, and present the strategies to enhance carbon fixation efficiency of the CBB cycle in heterotrophic microorganisms. Recent progress in such applications in reinforcing CO2 fixation for the improved production of various value-added chemicals is also summarized. The challenges encountered in this process and future prospects are further discussed.

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Section: Chemicals With the Cbb Cycle Equipped Strainsmentioning

confidence: 99%

Section: The Production Of Value-added Chemicals With the Cbb Cycle E...mentioning

confidence: 99%

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle

Feng

Gao

et al. 2023

ACS Sustainable Chem. Eng.

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“…The role of Rubisco in CO 2 assimilation through the Calvin-Benson-Bassham cycle (CBB) [150], besides giving rise to the 3PG pool for glycogen and PHB production, is also a possible route for photosynthetic PHB production, with the 2-phosphoglycolate (2PG) resulting from CBB producing glycolate that in turn can be used as a starting blinding block for PHB synthesis. This was observed in a recombinant Escherichia coli JW2946 [151]. As for the production of PHV in cyanobacteria, it uses propionic acid as a starting point, and can occur in conjunction with PHB biosynthesis, resulting in the PHBV copolymer [12].…”

Section: Pha Production In Cyanobacteriamentioning

confidence: 99%

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

2020

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Conventional petrochemical plastics have become a serious environmental problem. Its unbridled use, especially in non-durable goods, has generated an accumulation of waste that is difficult to measure, threatening aquatic and terrestrial ecosystems. The replacement of these plastics with cleaner alternatives, such as polyhydroxyalkanoates (PHA), can only be achieved by cost reductions in the production of microbial bioplastics, in order to compete with the very low costs of fossil fuel plastics. The biggest costs are carbon sources and nutrients, which can be appeased with the use of photosynthetic organisms, such as cyanobacteria, that have a minimum requirement for nutrients, and also using agro-industrial waste, such as the livestock industry, which in turn benefits from the by-products of PHA biotechnological production, for example pigments and nutrients. Circular economy can help solve the current problems in the search for a sustainable production of bioplastic: reducing production costs, reusing waste, mitigating CO2, promoting bioremediation and making better use of cyanobacteria metabolites in different industries.

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“…RuBisCO is a key enzyme in Calvin cycle and plays a central role in the carbon fixation of photosynthesis. It catalyzes the carboxylation of ribulose-1,5-bisphosphate to yield two molecules of 3-phosphoglycerate, and simultaneously oxidizes the pentose substrate in the photorespiration process [27]. It has been reported that soybean seed at physiological maturity stage contains some mature chloroplasts without any photosynthesis activity [21].…”

Section: Comparison Of Effects Of Hth Stress On Seed Vigor Formation mentioning

confidence: 99%

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

Liu

et al. 2020

BMC Plant Biol

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Background: Soybean developing seed is susceptible to high temperature and humidity (HTH) stress in the field, resulting in vigor reduction. Actually, the HTH in the field during soybean seed growth and development would also stress the whole plant, especially on leaf and pod, which in turn affect seed growth and development as well as vigor formation through nutrient supply and protection. Results: In the present study, using a pair of pre-harvest seed deterioration-sensitive and-resistant cultivars Ningzhen No. 1 and Xiangdou No. 3, the comprehensive effects of HTH stress on seed vigor formation during physiological maturity were investigated by analyzing cotyledon, embryo, leaf, and pod at the levels of protein, ultrastructure, and physiology and biochemistry. There were 247, 179, and 517 differentially abundant proteins (DAPs) identified in cotyledon, embryo, and leaf of cv. Xiangdou No. 3 under HTH stress, while 235, 366, and 479 DAPs were identified in cotyledon, embryo, and leaf of cv. Ningzhen No. 1. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Most of the DAPs identified were found to be involved in major metabolic pathways and cellular processes, including signal transduction, tricarboxylic acid cycle, fatty acid metabolism, photosynthesis, protein processing, folding and assembly, protein biosynthesis or degradation, plant-pathogen interaction, starch and sucrose metabolism, and oxidative stress response. The HTH stress had less negative effects on metabolic pathways, cell ultrastructure, and physiology and biochemistry in the four organs of Xiangdou No. 3 than in those of Ningzhen No. 1, leading to produce higher vigor seeds in the former.

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Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli

Cited by 13 publications

References 19 publications

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

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Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli

Cited by 13 publications

References 19 publications

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO2 Fixation Based on Calvin–Benson–Bassham Cycle

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO2 Fixation Based on Calvin–Benson–Bassham Cycle

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

Contact Info

Product

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Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO₂ Fixation Based on Calvin–Benson–Bassham Cycle