RIC-8 Is Required for GPR-1/2-Dependent Gα Function during Asymmetric Division of C. elegans Embryos

Afshar, Katayoun; Willard, Francis S.; Colombo, Kelly; Johnston, Christopher A.; McCudden, Christopher R.; Siderovski, David P.; Gönczy, Pierre

doi:10.1016/j.cell.2004.09.026

Cited by 183 publications

(272 citation statements)

References 37 publications

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“…It functions as a guanine nucleotide exchange factor (GEF) in different organisms like C. elegans, Drosophila melanogaster, and mammals Tall et al, 2003;Afshar et al, 2004;Couwenbergs et al, 2004;David et al, 2005). RIC-8 associates with GDPbound Ga proteins, stimulating the release of GDP and forms a stable nucleotide transition state complex, which is dissociated upon binding of GTP to Ga (Tall et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…It assists in the alignment of mitotic spindle, nuclear migration, and other centrosomemediated events during early embryogenesis in C. elegans Afshar et al, 2004Afshar et al, , 2005Couwenbergs et al, 2004;Wilkie and Kinch, 2005) and in the formation of neuroblasts and sensory precursor cells in Drosophila (David et al, 2005;Hampoelz et al, 2005;Wang et al, 2005). Biochemical studies of mammalian RIC-8 function suggest that it regulates the microtubule pulling forces on centrosomes during cell division (Tall and Gilman, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

Tõnissoo¹,

Lulla²,

Meier³

et al. 2010

Developmental Dynamics

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The guanine nucleotide exchange factor RIC-8 is a conserved protein essential for the asymmetric division in the early embryogenesis in different organisms. The function of RIC-8 in mammalian development is not characterized so far. In this study we map the expression of RIC-8 during the early development of mouse. To elucidate the RIC-8 function we used Ric-8 2/2 mutant embryos. The Ric-8 2/2 embryos reach the gastrulation stage but do not develop further and die at E6.5-E8.5. We characterized the Ric-8 2/2 embryonic phenotype by morphological and marker gene analyses. The gastrulation is initiated in Ric-8 2/2 embryos but their growth is retarded, epiblast and mesoderm disorganized. Additionally, the basement membrane is defective, amnion folding and the formation of allantois are interfered, also the cavitation. Furthermore, the orientation of the Ric-8 2/2 embryo in the uterus was abnormal. Our study reveals that the activity of RIC-8 protein is irreplaceable for the correct gastrulation of mouse embryo. Developmental Dynamics 239:3404-3415,

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

Tõnissoo¹,

Lulla²,

Meier³

et al. 2010

Developmental Dynamics

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“…Experiments using D2-receptor expressing COS-7 membranes were incubated for 60 min at 22°C. Steady-state GTP hydrolysis assays were carried out as described (32). Reactions were otherwise carried out under conditions identical to [ 35 S]GTP␥S binding assays.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Structural basis for nucleotide exchange on Gα _i subunits and receptor coupling specificity

Johnston

Siderovski

2007

Proc. Natl. Acad. Sci. U.S.A.

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The authors wish to note the following: "In our paper, a cocrystal structure at 2.2 Å resolution was described of the heterotrimeric G-protein alpha subunit Gαi1 bound to two peptides: one from an artificial sequence that promotes nucleotide exchange (KB-752) and a second peptide (D2N) from the third intracellular loop of the D2 dopamine receptor (PDB ID code 2HLB). Further examination of the unbiased electron density map has revealed that, while electron density exists for the KB-752 peptide, there is a lack of clear and continuous electron density for the D2N receptor peptide in the complex. Because the structural model represents a major conclusion of the paper but is unsupported by the experimental electron density map, we wish to retract the paper. Both authors deeply regret this mistake and sincerely apologize."

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“…Alternative models describe the interplay of Ric-8, RGS, GoLoco, G␣, and their accessory proteins in regulating aster microtubule forces during cell division (3,(8)(9)(10)(11). One model predicts that GoLoco protein-G␣-GDP is the active complex that regulates the microtubule pulling forces.…”

mentioning

confidence: 99%

Resistance to inhibitors of cholinesterase 8A catalyzes release of Gαi-GTP and nuclear mitotic apparatus protein (NuMA) from NuMA/LGN/Gαi-GDP complexes

Tall

Gilman

2005

Proc. Natl. Acad. Sci. U.S.A.

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Resistance to inhibitors of cholinesterase (Ric) 8A is a guanine nucleotide exchange factor that activates certain G protein ␣-subunits. Genetic studies in Caenorhabditis elegans and Drosophila melanogaster have placed RIC-8 in a previously uncharacterized G protein signaling pathway that regulates centrosome movements during cell division. Components of this pathway include G protein subunits of the G␣i class, GPR or GoLoco domain-containing proteins, RGS (regulator of G protein signaling) proteins, and accessory factors. These proteins interact to regulate microtubule pulling forces during mitotic movement of chromosomes. It is unclear how the GTP-binding and hydrolysis cycle of G␣i functions in the context of this pathway. In mammals, the GoLoco domain-containing protein LGN (GPSM2), the LGN-and microtubule-binding nuclear mitotic apparatus protein (NuMA), and G␣i regulate a similar process. We find that mammalian Ric-8A dissociates G␣i-GDP͞LGN͞ NuMA complexes catalytically, releasing activated G␣i-GTP in vitro. Ric-8A-stimulated activation of G␣i caused concomitant liberation of NuMA from LGN. We conclude that Ric-8A efficiently utilizes GoLoco͞G␣i-GDP complexes as substrates in vitro and suggest that Ric-8A-stimulated release of G␣i-GTP and͞or NuMA regulates the microtubule pulling forces on centrosomes during cell division.cell division ͉ G protein ͉ GoLoco ͉ GPR ͉ guanine nucleotide exchange

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RIC-8 Is Required for GPR-1/2-Dependent Gα Function during Asymmetric Division of C. elegans Embryos

Cited by 183 publications

References 37 publications

Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

RETRACTED: Structural basis for nucleotide exchange on Gα _i subunits and receptor coupling specificity

Resistance to inhibitors of cholinesterase 8A catalyzes release of Gαi-GTP and nuclear mitotic apparatus protein (NuMA) from NuMA/LGN/Gαi-GDP complexes

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RIC-8 Is Required for GPR-1/2-Dependent Gα Function during Asymmetric Division of C. elegans Embryos

Cited by 183 publications

References 37 publications

Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

Nucleotide exchange factor RIC‐8 is indispensable in mammalian early development

RETRACTED: Structural basis for nucleotide exchange on Gα i subunits and receptor coupling specificity

Resistance to inhibitors of cholinesterase 8A catalyzes release of Gαi-GTP and nuclear mitotic apparatus protein (NuMA) from NuMA/LGN/Gαi-GDP complexes

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RETRACTED: Structural basis for nucleotide exchange on Gα _i subunits and receptor coupling specificity