Rice cell suspension culture as a model for producing high-value recombinant proteins and plant specialized metabolites

Arya, Sagar S.; Kumari, Divyani; Rookes, James; Cahill, David M.; Lenka, Sangram K.

doi:10.1007/s11240-021-02028-z

Cited by 8 publications

(4 citation statements)

References 182 publications

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“…Transcriptome profiling of an organism and identification of genes responsive towards production of plant secondary metabolite can be obtained through microarray assay [ 63 ]. For instance, rice cell suspension culture (RCSC) treated with abscisic acid (ABA) inspected for real time proteomics and metabolomics profiling helped in evaluating the effect of the physicochemical conditions [ 67 ]. Likewise, using transcriptomic analysis Taxus cell culture was evaluated for genes associated with enzymatic synthesis related with taxane production [ 68 ].…”

Section: Future Prospectsmentioning

confidence: 99%

“…For improving the quality of recombinant IgG in Nicotiana tabacum BY-2 cells CRISPR multiplexing was done on two β-(1,2)-xylosyl transferase ( XylT ) genes and four α-(1,3)-fucosyl transferase ( FucT ) genes respectively. Furthermore, addition of heterologous genes in rice cell suspension culture (RCSC) applied through CRISPR/Cas mediated insertion/replacement led to the production of novel Phyto chemicals [ 67 ]. In addition to this, knocking out of the rosmarinic acid synthase gene and diterpene synthase gene in Salvia miltiorrhiza has shown a decline in the formation of rosmarinic acid and lithospermic acid B and tanshione [ 76 ].…”

Section: Future Prospectsmentioning

confidence: 99%

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Eco-friendly approaches to phytochemical production: elicitation and beyond

Jalota,

Sharma,

Agarwal

et al. 2024

Nat. Prod. Bioprospect.

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Highly ameliorated phytochemicals from plants are recognized to have numerous beneficial effects on human health. However, obtaining secondary metabolites directly from wild plants is posing a great threat to endangered plant species due to their over exploitation. Moreover, due to complicated structure and stereospecificity chemical synthesis of these compounds is a troublesome procedure. As a result, sustainable and ecofriendly in vitro strategy has been adopted for phytochemicals production. But, lack of fully differentiated cells lowers down cultured cells productivity. Consequently, for enhancing yield of metabolites produced by cultured plant cells a variety of methodologies has been followed one such approach includes elicitation of culture medium that provoke stress responses in plants enhancing synthesis and storage of bioactive compounds. Nevertheless, for conclusive breakthrough in synthesizing bioactive compounds at commercial level in-depth knowledge regarding metabolic responses to elicitation in plant cell cultures is needed. However, technological advancement has led to development of molecular based approaches like metabolic engineering and synthetic biology which can serve as promising path for phytochemicals synthesis. This review article deals with classification, stimulating effect of elicitors on cultured cells, parameters of elicitors and action mechanism in plants, modern approaches like metabolic engineering for future advances. Graphical Abstract

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Section: Future Prospectsmentioning

confidence: 99%

Section: Future Prospectsmentioning

confidence: 99%

Eco-friendly approaches to phytochemical production: elicitation and beyond

Jalota,

Sharma,

Agarwal

et al. 2024

Nat. Prod. Bioprospect.

View full text Add to dashboard Cite

show abstract

“…As in vitro culture technology improves by leaps and bounds, the past years have seen an increase in using plants that produce bioactive substances to cultivate suspension cells, such as Daucus carota , Nicotiana tabacum , Oryza sativa , Glycine max , and Lycopersicon esculentum , and transgenic Daucus carota and Nicotiana tabacum cell lines have been used as commercial plant cell platforms. Many dicotyledons have obtained stable suspension cell systems, mainly concentrating in plants with medicinal activities, and there are many reports of Labiatae, such as Scutellaria baicalensis , Perilla frutescens , Salvia miltiorrhiza , Mentha piperita , Leonurus cardiaca , Agastache rugosa , etc.…”

Section: Suspension Cell Systems Of Plantsmentioning

confidence: 99%

Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

Jia

Yan

et al. 2022

J. Agric. Food Chem.

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Plants can produce many functional bioactive substances. The suspension cell system of plants can be constructed based on its characteristics to realize the large-scale production of valuable products. In this review, we mainly talk about the main strategies, elicitation, and genetic transformation to improve the yield of active substances by using this system. Meanwhile, we focus on the challenges hiding in the practical application and the future prospects and provide new ideas and the theoretical basis for obtaining numerous bioactive substances from plants.

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“…In subsequent studies, canthaxanthin rice and astaxanthin rice appeared one after another, making rice a dietary supplement to meet daily nutritional needs ( Zhu et al., 2018 ). In addition, rice cell suspension culture is becoming a model for the production of specialized plant metabolites ( Arya et al., 2021 ).…”

Section: Engineered Biosynthesis Of Pnps In Plant Cellsmentioning

confidence: 99%

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

Zhu

Liu

et al. 2021

Plant Communications

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Plant natural products (PNPs) are the main sources of drugs, food additives, and new biofuels and have become a hotspot in synthetic biology. In the past two decades, the engineered biosynthesis of many PNPs has been achieved through the construction of microbial cell factories. Alongside the rapid development of plant physiology, genetics, and plant genetic modification techniques, hosts have now expanded from single-celled microbes to complex plant systems. Plant synthetic biology is an emerging field that combines engineering principles with plant biology. In this review, we introduce recent advances in the biosynthetic pathway elucidation of PNPs and summarize the progress of engineered PNP biosynthesis in plant cells. Furthermore, a future vision of plant synthetic biology is proposed. Although we are still a long way from overcoming all the bottlenecks in plant synthetic biology, the ascent of this field is expected to provide a huge opportunity for future agriculture and industry.

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Rice cell suspension culture as a model for producing high-value recombinant proteins and plant specialized metabolites

Cited by 8 publications

References 182 publications

Eco-friendly approaches to phytochemical production: elicitation and beyond

Eco-friendly approaches to phytochemical production: elicitation and beyond

Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

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