Rice F-bZIP transcription factors regulate the zinc deficiency response

Abstract

The F-bZIP transcription factors bZIP19 and bZIP23 are the central regulators of the zinc deficiency response in Arabidopsis, and phylogenetic analysis of F-bZIP homologs across land plants indicates that the regulatory mechanism of the zinc deficiency response may be conserved. Here, we identified the rice F-bZIP homologs and investigated their function. OsbZIP48 and OsbZIP50, but not OsbZIP49, complement the zinc deficiency-hypersensitive Arabidopsis bzip19bzip23 double mutant. Ectopic expression of OsbZIP50 in Arabidopsis significantly increases plant zinc accumulation under control zinc supply, suggesting an altered Zn sensing in OsbZIP50. In addition, we performed a phylogenetic analysis of F-bZIP homologs from representative monocot species that supports the branching of plant F-bZIPs into Group 1 and Group 2. Our results suggest that regulation of the zinc deficiency response in rice is conserved, with OsbZIP48 being a functional homolog of AtbZIP19 and AtbZIP23. A better understanding of the mechanisms behind the Zn deficiency response in rice and other important crops will contribute to develop plant-based strategies to address the problems of Zn deficiency in soils, crops, and cereal-based human diets.

Structure, Function, Regulation and Phylogenetic Relationship of ZIP Family Transporters of Plants

“…Among these, the TFs HvbZIP56 and HvbZIP62 could restore the growth of bzip19 and bzip23 double mutant of Arabidopsis under Zn deficiency condition (Nazri et al, 2017). Recently, Lilay et al (2020) identified and characterized three bZIP TFs in rice such as OsbZIP48, OsbZIP49, and OsbZIP50. The OsbZIP48 and OsbZIP50 complemented the Arabidopsis double mutant (bzip19/bzip23) under Zn deficient condition but the OsbZIP49 does not compliment the Arabidopsis double mutant (bzip19/bzip23) (Lilay et al, 2020).…”

“…Recently, Lilay et al (2020) identified and characterized three bZIP TFs in rice such as OsbZIP48, OsbZIP49, and OsbZIP50. The OsbZIP48 and OsbZIP50 complemented the Arabidopsis double mutant (bzip19/bzip23) under Zn deficient condition but the OsbZIP49 does not compliment the Arabidopsis double mutant (bzip19/bzip23) (Lilay et al, 2020).…”

Comparative efficacy of selenate and selenium nanoparticles for improving growth, productivity, fruit quality, and postharvest longevity through modifying nutrition, metabolism, and gene expression in tomato; potential benefits and risk assessment

“…The basic leucine zipper (bZIP) transcription factors are involved in a plethora of plant biological processes and responses, including signal transduction [ 6 , 13 ], tissue differentiation [ 13 ], hormone signaling [ 14 ], nutrition [ 15 ], and stress tolerance [ 6 , 16 ]. For this reason, evaluating the potential nSe-associated alteration in the bZIP transcription factor was also one of the aims of this study.…”

Regulation Mechanisms of Plant Basic Leucine Zippers to Various Abiotic Stresses

“…To date, plenty of literatures show that bZIPs regulate plant tolerances to various abiotic stresses, such as low temperature, drought, high salt, nitrogen deficiency, zinc deficiency time ( Lilay et al, 2020 ; Ueda et al, 2020 ). Besides, there are many reports reveal that flavonoids participate in various stress responses.…”