Rice: Genetic Engineering Approaches for Abiotic Stress Tolerance – Retrospects and Prospects

Singh, S. S.; Modi, Mahendra Kumar; Gill, Sarvajeet Singh; Tuteja, Narendra

doi:10.1002/9783527665334.ch10

Cited by 5 publications

(4 citation statements)

References 175 publications

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“…The reduction in chlorophyll content has been reported under salinity stress in various crop plants [22,31,43]. However, the PDH45 overexpressing transgenic events retained more chlorophyll than WT under salinity stress, which has strong correlation with the salinity tolerance potential [12,26,27,30].…”

Section: Discussionmentioning

confidence: 96%

Introduction of Pea DNA Helicase 45 into Sugarcane (Saccharum spp. Hybrid) Enhances Cell Membrane Thermostability and Upregulation of Stress-Responsive Genes Leads to Abiotic Stress Tolerance

et al. 2015

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DNA helicases are motor proteins that play an essential role in nucleic acid metabolism, by providing a duplex-unwinding function. To improve the drought and salinity tolerance of sugarcane, a DEAD-box helicase gene isolated from pea with a constitutive promoter, Port Ubi 2.3 was transformed into the commercial sugarcane variety Co 86032 through Agrobacterium-mediated transformation, and the transgenics were screened for tolerance to soil moisture stress and salinity. The transgene integration was confirmed through polymerase chain reaction, and the V 0 transgenic events showed significantly higher cell membrane thermostability under normal irrigated conditions. The V 1 transgenic events were screened for tolerance to soil moisture stress and exhibited significantly higher cell membrane thermostability, transgene expression, relative water content, gas exchange parameters, chlorophyll content, and photosynthetic efficiency under soil moisture stress compared to wild-type (WT). The overexpression of PDH45 transgenic sugarcane also led to the upregulation of DREB2-induced downstream stress-related genes. The transgenic events demonstrated higher germination ability and better chlorophyll retention than WT under salinity stress. Our results suggest the possibility for development of increased abiotic stress tolerant sugarcane cultivars through overexpression of PDH45 gene. Perhaps this is the first report, which provides evidence for increased drought and salinity tolerance in sugarcane through overexpression of PDH45.

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Section: Discussionmentioning

confidence: 96%

Introduction of Pea DNA Helicase 45 into Sugarcane (Saccharum spp. Hybrid) Enhances Cell Membrane Thermostability and Upregulation of Stress-Responsive Genes Leads to Abiotic Stress Tolerance

et al. 2015

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“…Genetic divergence is extremely important and key for crop advancement. Existence of additional variability in the main population increases possibility of more enhancement (2). Phenotypic assessment can contribute additional information on the structure and spatial distribution of diversification.…”

Section: Results and Disscusionmentioning

confidence: 99%

“…Rice is vulnerable to many biotic and abiotic stresses like insect-pest, fungus, viruses, drought, flood, submergence, high/low temperature, salinity, iron (Fe) toxicity [1]. These factors result 30-60% yield loss globally in each year [2]. Iron toxicity is a serious nutrient disorder in lowland areas.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Assessment of Natural Diversity in Low-Land Rice Germplasm as Affected by Iron Toxicity

Saha

Mohanty

Panda

et al. 2020

CJAST

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Iron toxicity that seriously affect rice yield is a critical concern for the crop improvement programs in rice. Morphological analysis of germplasm is essential for the success of varietal crop improvement programs. The objective of this investigation is to estimate the phenotypic diversification of one hundred and fifty germplasm to identify the tolerant genotypes under iron toxic situation for exploitation of inherited variability from the accessible germplasm. Experiment has been carried out under lowland field condition to determine the reaction of rice germplasm under hotspots for iron toxicity. Significant differences among the genotypes have been observed. A significant difference is present among all the traits like days to 50% flowering, Plant height, panicle length, grain/panicle, grain weight, yield, leaf bronzing index (LBI) and tillers number. The phylogenetic analysis was also carried out to find out a core population for further study like association mapping with trait of interest. The genotypes like Mahsuri, Kusuma, Ganjamgedi, Pratikhya, Swarna, Dhusura have been found to be tolerant genotypes under iron toxic condition.

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“…Rice is the main staple crop and provides essential daily calories to over half of the world population. 1,2 In recent era, helicases have evolved as an important and emerging player in the abiotic stress tolerance, including salinity stress. [3][4][5][6][7][8][9] Among helicases, pea DNA helicase, PDH45 is a unique member, which contains DESD and SRT instead of the typical DEAD/H and SAT in motifs V and VI, respectively.…”

Section: Introductionmentioning

confidence: 99%

PDH45overexpressing transgenic tobacco and rice plants provide salinity stress tolerance via less sodium accumulation

Nath

Garg

Sahoo

et al. 2015

Plant Signaling & Behavior

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Keywords: CoroNa Green dye, pea DNA helicase 45 (PDH45), salinity stress tolerance, sodium ions accumulation, transgenic tobacco and rice Salinity stress negatively affects the crop productivity worldwide, including that of rice. Coping with these losses is a major concern for all countries. The pea DNA helicase, PDH45 is a unique member of helicase family involved in the salinity stress tolerance. However, the exact mechanism of the PDH45 in salinity stress tolerance is yet to be established. Therefore, the present study was conducted to investigate the mechanism of PDH45-mediated salinity stress tolerance in transgenic tobacco and rice lines along with wild type (WT) plants using CoroNa Green dye based sodium localization in root and shoot sections. The results showed that under salinity stress root and shoot of PDH45 overexpressing transgenic tobacco and rice accumulated less sodium (Na C ) as compared to their respective WT. The present study also reports salinity tolerant (FL478) and salinity susceptible (Pusa-44) varieties of rice accumulated lowest and highest Na C level, respectively. All the varieties and transgenic lines of rice accumulate differential Na C ions in root and shoot. However, roots accumulate high Na C as compared to the shoots in both tobacco and rice transgenic lines suggesting that the Na C transport in shoot is somehow inhibited. It is proposed that the PDH45 is probably involved in the deposition of apoplastic hydrophobic barriers and consequently inhibit Na C transport to shoot and therefore confers salinity stress tolerance to PDH45 overexpressing transgenic lines. This study concludes that tobacco (dicot) and rice (monocot) transgenic plants probably share common salinity tolerance mechanism mediated by PDH45 gene.

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Rice: Genetic Engineering Approaches for Abiotic Stress Tolerance – Retrospects and Prospects

Cited by 5 publications

References 175 publications

Introduction of Pea DNA Helicase 45 into Sugarcane (Saccharum spp. Hybrid) Enhances Cell Membrane Thermostability and Upregulation of Stress-Responsive Genes Leads to Abiotic Stress Tolerance

Introduction of Pea DNA Helicase 45 into Sugarcane (Saccharum spp. Hybrid) Enhances Cell Membrane Thermostability and Upregulation of Stress-Responsive Genes Leads to Abiotic Stress Tolerance

Phenotypic Assessment of Natural Diversity in Low-Land Rice Germplasm as Affected by Iron Toxicity

PDH45overexpressing transgenic tobacco and rice plants provide salinity stress tolerance via less sodium accumulation

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