Rice (Oryza sativa L.) tau class glutathione S-transferase (OsGSTU30) overexpression in Arabidopsis thaliana modulates a regulatory network leading to heavy metal and drought stress tolerance

Srivastava, Dipali; Verma, G. S.; Chauhan, Abhishek; Pande, Veena; Chakrabarty, Debasis

doi:10.1039/c8mt00204e

Cited by 87 publications

(40 citation statements)

References 55 publications

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“…). Cr(VI) is a toxic metal and after its entry into plants it initiates a ranges of unfavorable changes (Eleftheriou et al , Pokorska‐Niewiada et al , Sinha et al , Srivastava et al ) and thus, the obtained results are justifiable. We have also noticed that Cr(VI) significantly declined qP and the amount of total chlorophyll (Table , Fig.…”

Section: Discussionmentioning

confidence: 96%

“…However, it has been reported that Cr(VI) is transported through sulfate transporters in plants (Kaszycki et al 2005, Kim et al 2006. After its entry in the plant system, Cr(VI) creates wide ranges of physiological, biochemical and molecular alterations starting from seed germination to plant death (Eleftheriou et al 2015, Pokorska-Niewiada et al 2018, Sinha et al 2018, Srivastava et al 2019. Thus, continuous investigation of the toxicological impact of Cr(VI) in plants is needed in order to develop strategies for its toxicity mitigation.…”

Section: Introductionmentioning

confidence: 99%

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Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Kushwaha

Singh

2019

Physiologia Plantarum

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In plants, investigation on heavy metal toxicity and its mitigation by nutrient elements have gained much attention. However, mechanism(s) associated with nutrients‐mediated mitigation of metal toxicity remain elusive. In this study, we have investigated the role and interrelation of glutathione (GSH) and hydrogen sulfide (H2S) in the regulation of hexavalent chromium [Cr(VI)] toxicity in tomato (Solanum lycopersicum), pea (Pisum sativum) and brinjal (Solanum melongena) seedlings, supplemented with additional sulfur (S). The results show that Cr(VI) significantly reduced growth, total chlorophyll and photosynthetic quantum yield of tomato, pea and brinjal seedlings which was accompanied by enhanced intracellular accumulation of Cr(VI) in roots. Moreover, Cr(VI) enhanced the generation of reactive oxygen species in the studied vegetables, while antioxidant defense system exhibited differential responses. However, additional supply of S alleviated Cr(VI) toxicity. Interestingly, addition of l‐buthionine sulfoximine (BSO, a glutathione biosynthesis inhibitor) further increased Cr(VI) toxicity even in the presence of additional S but GSH addition reverses the effect of BSO. Under similar condition, endogenous H2S, l‐cysteine desulfhydrase (DES) activity and cysteine content did not significantly differ when compared to controls. Hydroxylamine (HA, an inhibitor of DES) also increased Cr(VI) toxicity even in the presence of additional S but sodium hydrosulfide (NaHS, an H2S donor) reverses the effect of HA. Moreover, Cr(VI) toxicity amelioration by NaHS was reversed by the addition of hypotaurine (HT, an H2S scavenger). Taken together, the results show that GSH which might be derived from supplied S is involved in the mitigation of Cr(VI) toxicity in which H2S signaling preceded GSH biosynthesis.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Kushwaha

Singh

2019

Physiologia Plantarum

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“…Therefore, we hypothesize that MT2a may not participate in Cd detoxi cation in grey poplar. GST expression is induced by a wide range of biotic and abiotic stresses, including pathogens, heavy metals, drought, salt or hormones [64][65][66][67]. In Oryza sativa L., the GST proteins might alleviate Cu toxicity by the binding of free Cu 2+ in cells [68], which might be an important mechanism in defence towards heavy metal toxicity.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Transcriptomic Response of Grey Poplar (Populus ×Canescens Aiton Sm.) to Cadmium Stress

Komarkova

Chromy

Pokorná

et al. 2020

Preprint

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Background: Populus ×canescens (Aiton) Sm. is a fast-growing woody plant belonging to the family Salicaceae. Thanks to its deep root system and fast growth rate, two poplar genotypes (TP11 and TP20) was chosen to be characterized and tested for a physiological and transcriptomic response to Cd stress.Results: Both sexual and clonal reproduction are common in grey poplar. Thus, to reveal its genetic structure, two selected poplar genotypes characterized by unique phenotypic traits were distinguished by microsatellite analysis. A comparative analysis of the effects of exposure to high cadmium (Cd) concentrations on the physiological response of TP11 and TP20 was performed. After 2 and 10 days, 10 µM and 100 µM Cd were added to the nutrient solution of poplar plants grown in perlite. Neither tested Cd concentration negatively affected plant growth; however, the chlorophyll content significantly decreased. According to mineral uptake analysis, the potassium (K) content was higher in the shoots than in the roots. The calcium (Ca) and magnesium (Mg) concentrations were only slightly affected by Cd treatment. The zinc (Zn) content in the shoots of TP20 was lower than that in the shoots of TP11. Moreover, the amount of Zn was slightly higher in the shoots of both genotypes after 10 days of Cd treatment compared to the control conditions. Cd accumulation was higher in the roots than in the shoots. After 2 days of 100 µM Cd treatment, TP11 accumulated significantly more Cd in the roots than TP20. After 10 days of exposure, 10 µM Cd resulted in comparable amounts of Cd in the roots and shoots of TP20, which was reflected in a translocation factor (TF) value greater than 1. Quantitative real-time RT-PCR analyses were used to examine the expression profiles of selected stress-response genes in the roots and shoots of plants exposed to Cd. The most significant change in transcript amount was observed in endochitinase 2, 12-oxophytodienoate reductase 1 and phi class glutathione S-transferase, which have been characterized as stress-response genes.Conclusions: Our study provided new insights for effective assessing the ability of different poplar genotypes to tolerate Cd stress and underlying Cd tolerance.

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“…The high-throughput technologies, next generation sequencing (NGS) i.e., ABI/SOLiD, Roche/454, Illumina/Solexa has made it actually possible to produce genome resources at a sizable level and comparatively low cost [12,13]. The next-generation sequencing have already been e ciently utilized to create largescale transcriptome details in a number of plant species, i.e., Rice [14] Arabidopsis [15,16], wheat [17], barley [18], and maize [19] to identify the candidate genes mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Insight of Transcriptional Regulators reveals the Tolerance Mechanism of Carpet-grass (Axonopus compressus) Against Drought

Nawaz

Liao

Azeem

et al. 2020

Preprint

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Highlights:1. Axonopus compressus can stand severe drought stress by activating the potential defense mechanism.2. We investigated the differential transcriptome of drought stressed and normal Axonopus compressus plants3. New comers have been identified that involved in the drought response4. Identified drought responsive genes which never known for other stresses.5. The identified genes also respond to stress in Arabidopsis thaliana in different manners.Background: Carpet grass [Axonopus compressus (L.)] is an important warm season perennial grass around the world and is renowned for its adaptability to varied environmental conditions. However, Carpet grass lacks enough data in public data bank, which confined our comprehension of the mechanism of environmental adaptations, gene discovery and development of molecular marker. Methods: In current study, the DEGs (differentially expressed genes) in Axonopus compressus under drought stress (DS) were identified and compared with CK (control) by RNA-Seq. Results: the 263,835 of total unigenes were identified in Axonopus compressus, and 201,303 (also add the numbers of remaining2 data bases) a sequence of unigenes significantly matched in at least one of the seven databases. A total of 153697 (58.25%) unigenes can be classified to 144 KEGG pathways, and 7,444 unigenes were expressed differentially between DS and CK, of which 4,249 were up-regulated and 3,195 were down-regulated unigenes. Of the 50 significantly enriched GO terms, 18, 6 and 14 items were related to BP, CC and MF, respectively. Analysis of KEGG enrichment showed 2569 DEGs involved in 143 different pathways, under drought stress 2,747 DEGs were up-regulated and 2,502 DEGs were down-regulated. Moreover, we identified 352 transcriptor factors (TFs) in Axonopus compressus, of which 270 were differentially expressed between CK and DS. The qRT-PCR validation experiment also support the transcriptional response of Axonopus compressus against drought. Conclusions: The current findings provide the first framework for further investigation for the particular roles of these unigenes in Axonopus compressus in response to droughts.

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Rice (Oryza sativa L.) tau class glutathione S-transferase (OsGSTU30) overexpression in Arabidopsis thaliana modulates a regulatory network leading to heavy metal and drought stress tolerance

Abstract: OsGSTU30 increases the abiotic stress tolerance in plants either by its catalytic activity or by modulating the expression of stress responsive genes.

Cited by 87 publications

References 55 publications

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Physiological and Transcriptomic Response of Grey Poplar (Populus ×Canescens Aiton Sm.) to Cadmium Stress

Insight of Transcriptional Regulators reveals the Tolerance Mechanism of Carpet-grass (Axonopus compressus) Against Drought

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