Rice <i>Pi-ta</i> gene Confers Resistance to the Major Pathotypes of the Rice Blast Fungus in the United States

Jia, Yulin; Wang, Zhonghua; Fjellstrom, Robert G.; Moldenhauer, K. A. K.; Azam, Aa Haeruman; Correll, James C.; Lee, Fleet N.; Xia, Yingwu; Rutger, J. N.

doi:10.1094/phyto.2004.94.3.296

Cited by 85 publications

(60 citation statements)

References 38 publications

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“…Its cognate, AVR-Pita of M. grisea, is a putative metalloprotease possessing properties similar to bacterial effector proteins . Rice cultivars carrying the resistant Pi-ta allele have been confirmed in the Chinese field isolate O-137 , American field isolates ZN57 and ZN67 (Jia et al 2004), and Philippine field isolate IK81-25 (Fukuta et al 2007). This means that Pi-ta can recognize some AVR-pita variations from field isolates.…”

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confidence: 83%

Molecular Evolution of thePi-taGene Resistant to Rice Blast in Wild Rice (Oryza rufipogon)

et al. 2008

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Rice blast disease resistance to the fungal pathogen Magnaporthe grisea is triggered by a physical interaction between the protein products of the host R (resistance) gene, Pi-ta, and the pathogen Avr (avirulence) gene, AVR-pita. The genotype variation and resistant/susceptible phenotype at the Pi-ta locus of wild rice (Oryza rufipogon), the ancestor of cultivated rice (O. sativa), was surveyed in 36 locations worldwide to study the molecular evolution and functional adaptation of the Pi-ta gene. The low nucleotide polymorphism of the Pi-ta gene of O. rufipogon was similar to that of O. sativa, but greatly differed from what has been reported for other O. rufipogon genes. The haplotypes can be subdivided into two divergent haplogroups named H1 and H2. H1 is derived from H2, with nearly no variation and at a low frequency. H2 is common and is the ancestral form. The leucine-rich repeat (LRR) domain has a high p non /p syn ratio, and the low polymorphism of the Pi-ta gene might have primarily been caused by recurrent selective sweep and constraint by other putative physiological functions. Meanwhile, we provide data to show that the amino acid Ala-918 of H1 in the LRR domain has a close relationship with the resistant phenotype. H1 might have recently arisen during rice domestication and may be associated with the scenario of a blast pathogen-host shift from Italian millet to rice.

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confidence: 83%

Molecular Evolution of thePi-taGene Resistant to Rice Blast in Wild Rice (Oryza rufipogon)

et al. 2008

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“…A single amino acid substitution, serine (Ser) to alanine (Ala) at the position of 918, in the LRD of the Pi-ta protein was demonstrated to determine the direct interaction with AVR-Pita and the resistance specificity to blast pathogen M. oryazae Jia et al 2000). The resistant Pi-ta allele (Ala-918) was found in O. sativa and its ancestor O. rufipogon ( Jia et al 2004b;Huang et al 2008). Surveys of Pi-ta nucleotide sequences with limited accessions of Oryza species have revealed that the degree of nucleotide diversity is higher at the intron of the Pi-ta gene Huang et al 2008;Wang et al 2008;Yoshida and Miyashita 2009).…”

Section: P Lant Resistance (R) Genes Have Evolved To Fightmentioning

confidence: 99%

Evolutionary Dynamics of the Genomic Region Around the Blast Resistance Gene Pi-ta in AA Genome Oryza Species

et al. 2009

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The race-specific resistance gene Pi-ta has been effectively used to control blast disease, one of the most destructive plant diseases worldwide. A single amino acid change at the 918 position of the Pi-ta protein was known to determine resistance specificity. To understand the evolutionary dynamics present, we examined sequences of the Pi-ta locus and its flanking regions in 159 accessions composed of seven AA genome Oryza species: O. sativa, O. rufipogon, O. nivara, O. meridionalis, O. glaberrima, O. barthii, and O. glumaepatula. A 3364-bp fragment encoding a predicted transposon was found in the proximity of the Pi-ta promoter region associated with the resistance phenotype. Haplotype network analysis with 33 newly identified Pi-ta haplotypes and 18 newly identified Pi-ta protein variants demonstrated the evolutionary relationships of Pi-ta haplotypes between O. sativa and O. rufipogon. In O. rufipogon, the recent directional selection was found in the Pi-ta region, while significant deviation from neutral evolution was not found in all O. sativa groups. Results of sequence variation in flanking regions around Pi-ta in O. sativa suggest that the size of the resistant Pi-ta introgressed block was at least 5.4 Mb in all elite resistant cultivars but not in the cultivars without Pi-ta. These findings demonstrate that the Pi-ta region with transposon and additional plant modifiers has evolved under an extensive selection pressure during crop breeding.

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“…Tetep and Taducan) for the control of infection by races of M. oryzae that contain AVR-Pita (Jia and Martin, 2008;Kiyosawa, 1967;Moldenhauer et al, 1990). Pi-ta locus has been effectively utilized to prevent blast disease for the past 40 years and is still effectively used in more rice cultivation areas (Kiyosawa 1967, Jia et al, 2004, Barman et al, 2004, Jia and Valent 2007. The results of our research supported by other researches, indicate the importance of Pi-ta gene for improving both rice blast resistance and grain quality at blast infection conditions.…”

Section: Discussionmentioning

confidence: 99%

Rice grain quality as affected by blast-causing agent (Pyricularia oryzae) along with a molecular analysis of blast resistance at Pi5 and Pi-ta loci

Ahmadikhah¹,

Khosravi²

2018

Aust J Crop Sci

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Blast disease is the most devastating disease of rice worldwide, which reduces both quantity and quality of rice production. To assess the effect of fungal agent of blast, Pyricularia oryzae, on some aspects of grain quality of rice, 31 rice cultivars (including 20 local landraces) were evaluated at seedling and post-harvest stages. The cultivars were grown in three replicates in a field surrounded by blast spreader cultivars and at appropriate times blast infection type, grain infection, 100-grain weight and grain quality traits (percentage of broken rice and number of chalky seeds per panicle) were evaluated. For tracking alleles of two major blast resistance genes (including Pi5 and Pi-ta) the respective primer pairs were applied in a polymerase chain reaction (PCR) on DNA of the studied cultivars. Evaluation of blast infection type at seedling stage showed that four cultivars were completely resistant, 4 cultivars moderately resistant and 23 cultivars mainly consisted of local landraces were susceptible. Fungal treatment had significant effects on percentages of grain infection and broken rice and not on number of chalky seeds or 100-grain weight. Rice genotypes had significant differences in grain infection and also in 100-grain weight and in two quality traits including percentage of broken rice and number of chalky seeds. Most local landraces showed highest grain infection (14.4-17.6%) compared to improved modern varieties with lowest grain infection (1.3-2.7%). Results also showed that genotype by fungal treatment interaction were only significant for grain infection and broken rice. All susceptible cultivars had higher broken rice relative to resistant cultivars (10.5-13.3% vs. 2.1-4.6%) after blast infection. Molecular survey showed that most susceptible local landraces carry Pi5 dominant allele, while high-yielding modern cultivars don't carry the allele, indicating that Pi5 does not play a significant role in blast resistance at this time. Furthermore, molecular survey showed that only three cultivars including a local cultivar (Tetep) and two modern ones (IR56, IR24) harbored Pi-ta allele, and Pi-ta explained a high part of variation of blast resistance (R 2 =53.7%). These results indicate the importance of Pi-ta gene for improving rice blast-resistance and grain quality at blast infection conditions.

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Rice Pi-ta gene Confers Resistance to the Major Pathotypes of the Rice Blast Fungus in the United States

Cited by 85 publications

References 38 publications

Molecular Evolution of thePi-taGene Resistant to Rice Blast in Wild Rice (Oryza rufipogon)

Molecular Evolution of thePi-taGene Resistant to Rice Blast in Wild Rice (Oryza rufipogon)

Evolutionary Dynamics of the Genomic Region Around the Blast Resistance Gene Pi-ta in AA Genome Oryza Species

Rice grain quality as affected by blast-causing agent (Pyricularia oryzae) along with a molecular analysis of blast resistance at Pi5 and Pi-ta loci

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