Rice Mutants and Genes Related to Organ Development, Morphogenesis and Physiological Traits

Kurata, Nori; Miyoshi, Keita; Nonomura, Ken Ichi; Yamazaki, Yukiko; Ito, Yukihiro

doi:10.1093/pcp/pci506

Cited by 103 publications

(55 citation statements)

References 140 publications

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“…As the most important agronomical plant, rice is recognized as a useful experimental model of monocot to study the mechanism of gene expression (Kurata et al, 2005). In rice, 23 A-type and six B-type response regulator (RR) genes had been identified (Jain et al, 2006;Ito and Kurata, 2006).…”

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confidence: 99%

Characteristic and Expression Analysis of a Metallothionein Gene, OsMT2b, Down-Regulated by Cytokinin Suggests Functions in Root Development and Seed Embryo Germination of Rice

et al. 2008

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Metallothioneins (MTs) are low molecular mass and cysteine-rich metal-binding proteins known to be mainly involved in maintaining metal homeostasis and stress responses. But, their functions in higher plant development are scarcely studied. Here, we characterized rice (Oryza sativa) METALLOTHIONEIN2b (OsMT2b) molecularly and found that its expression was down-regulated by cytokinins. OsMT2b was preferentially expressed in rice immature panicles, scutellum of germinating embryos, and primordium of lateral roots. In contrast with wild-type plants, OsMT2b-RNA interference (RNAi) transgenic plants had serious handicap in plant growth and root formation, whereas OsMT2b-overexpressing transformants were dwarfed and presented more adventitious roots and big lateral roots. The increased cytokinin levels in RNAi plants and decreased cytokinin levels in overexpressing plants were confirmed by high-performance liquid chromatography quantitative analysis in the roots of wild-type and transgenic plants. In RNAi plants, localization of isopentenyladenosine, a kind of endogenous cytokinin, in roots and germinating embryos expanded to the whole tissues, whereas in overexpressing plants, the isopentenyladenosine signals were very faint in the vascular tissues of roots and scutellum cells of germinating embryos. In vitro culture of embryos could largely resume the reduced germination frequency in RNAi plants but had no obvious change in overexpressing plants. Taken together, these results indicate a possible feedback regulation mechanism of OsMT2b to the level of endogenous cytokinins that is involved in root development and seed embryo germination of rice.

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confidence: 99%

Characteristic and Expression Analysis of a Metallothionein Gene, OsMT2b, Down-Regulated by Cytokinin Suggests Functions in Root Development and Seed Embryo Germination of Rice

et al. 2008

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“…As explained above, mutation induction has been used as a powerful alternative to generate desired novel genetic sources for particular plant characters. Currently, rice mutant resources include collections that were generated by insertional, radiation or chemical mutagenesis (Hirochika et al, 2004;Kurata et al, 2005), but only irradiated or chemically induced rice mutants have been proved to be very useful in plant breeding programs, providing excellent varieties in a direct or indirect way. However, there are only few public available mutant collections based on induced mutations, including an indica collection of 50,000 M3 and M4 mutants generated from IR64 at IRRI and a japonica collection of 6,000 mutants derived from Kinmanze and Taichung 65 at Kyushu University (Inst.…”

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confidence: 99%

Rice cv. Bahia mutagenized population: a new resource for rice breeding in the Mediterranean basin

Domingo

Andrés

Talón

2007

Span. j. agric. res.

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In the Mediterranean basin, the limited availability of rice genetic resources restricts the success of breeding programs. Although mutation induction is a powerful alternative to generate desired novel variations, current rice mutant resources have little relevance in the Mediterranean breeding programs that require well-adapted cultivars for maximum performance. In this work a genome-wide mutation induction has been used on rice cv Bahia, a japonica cultivar well adapted to the Mediterranean climate and cultural practices, to increase its genetic variability and to facilitate the generation and selection of interesting agronomical traits. Bahia mutant collections were generated by irradiating seed batches with either 30 Gy fast neutrons or 250 Gy gamma rays. After initial screening of M2 plants and followed by evaluation of M3 and M4 generations in open field, elite genotypes were selected. The selected lines carried morphological and physiological changes of agronomical interest such as earliness, culm height reduction, yield increase and spontaneous spots appearance in leaves. The mutants are useful as breeding materials for improving varieties in the Mediterranean.

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“…The high frequency of gametophytic mutants indicates that gametophytic genes contribute significantly to pollen development. Few rice genes involved in pollen formation have been cloned (for review, Kurata et al 2005), although many genes are required for gametogenesis in plants (for review, Wilson and Yang 2004). Our study will provide a novel resource for understanding pollen development in rice.…”

Section: Discussionmentioning

confidence: 98%

Identification of Mutants for Abnormal Pollen Development in Rice

et al. 2007

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To gain a comprehensive understanding of the genetic regulation of male gametogenesis, we screened and identified mutants for abnormal pollen development in rice (Oryza sativa L.). We gamma-irradiated spikelets of a japonica rice cultivar, Taichung 65, just before and after anthesis. Among 1,000 M 2 lines, 24 lines were identified as mutants for pollen sterility. Progeny tests in M 3 demonstrated that pollen sterility in 12 lines was controlled by single recessive genes, designated sps1-sps12 (sporophytic pollen sterility). In the remaining 12 lines, pollen semi-sterility was under the control of gametophytic genes, designated gps1-gps12 (gametophytic pollen sterility). Linkage analysis revealed that sps6, sps9, and sps12 were mapped on chromosomes 3, 9, and 7, respectively.

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Rice Mutants and Genes Related to Organ Development, Morphogenesis and Physiological Traits

Cited by 103 publications

References 140 publications

Characteristic and Expression Analysis of a Metallothionein Gene, OsMT2b, Down-Regulated by Cytokinin Suggests Functions in Root Development and Seed Embryo Germination of Rice

Characteristic and Expression Analysis of a Metallothionein Gene, OsMT2b, Down-Regulated by Cytokinin Suggests Functions in Root Development and Seed Embryo Germination of Rice

Rice cv. Bahia mutagenized population: a new resource for rice breeding in the Mediterranean basin

Identification of Mutants for Abnormal Pollen Development in Rice

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