Rice nicotianamine synthase localizes to particular vesicles for proper function

Nozoye, Tomoko; Tsunoda, Kyoko; Nagasaka, Seiji; Takahashi, Michiko; Kobayashi, Takanori; Nakanishi, Hiromi; Nishizawa, Naoko K.

doi:10.4161/psb.28660

Cited by 25 publications

(20 citation statements)

References 42 publications

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“…In fact, cellular localizations of dicot NAS and graminaceous plants were different; OsNAS2, ZmNAS1, and ZmNAS2 in graminaceous plants localized to the particular vesicles, while AtNAS1-4 localized to the cytoplasm. 24,43,44) The specific character of soybean NAS may cause the higher level of NA. Previously, we reported that large amounts of NA could be produced in Saccharomyces cerevisiae strain SCY4.…”

Section: Discussionmentioning

confidence: 99%

Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean

Nozoye

Kim

Kakei

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Iron (Fe) is an essential nutrient in both plants and humans. Fe deficiency on calcareous soil with low Fe availability is a major agricultural problem. Nicotianamine (NA) is one of the Fe chelator in plants, which is involved in metal translocation into seeds, and serves as an antihypertensive substance in humans. In this study, soybean plants overexpressing the barley NA synthase 1 (HvNAS1) gene driven by the constitutive CaMV 35S promoter were produced using Agrobacterium-mediated transformation. The transgenic soybean showed no growth defect and grew normally. The NA content of transgenic soybean seeds was up to four-fold greater than that of non-transgenic (NT) soybean seeds. The level of HvNAS1 expression was positively correlated with the amount of NA, and a high concentration of NA was maintained in the seeds in succeeding generations. The Fe concentration was approximately two-fold greater in transgenic soybean seeds than in NT soybean seeds. Furthermore, the transgenic soybeans showed tolerance to low Fe availability in calcareous soil. Our results suggested that increasing the NA content in soybean seeds by the overexpression of HvNAS1 offers potential benefits for both human health and agricultural productivity.

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Section: Discussionmentioning

confidence: 99%

Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean

Nozoye

Kim

Kakei

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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“…The genes involved in DMA synthesis have been cloned and characterized (Bashir et al [ 2006 ]; Bashir and Nishizawa [ 2006 ]; Inoue et al [ 2003 ]; Inoue et al [ 2008 ]; Nozoye et al [ 2004 ]; Suzuki et al [ 2006 ]; Suzuki et al [ 2008 ]; Suzuki et al [ 2012 ]; Takahashi et al [ 1999 ]). Specifically, L-methionine is converted to nicotianamine (NA) by NA synthase 1-3 (OsNAS1-3), and is then converted to 3'-keto acid by NA aminotransferase 1 (OsNAAT1) and finally DMA synthase (OsDMAS1) converts this 3'-keto acid to DMA (Bashir et al [ 2006 ]; Bashir and Nishizawa [ 2006 ]; Bashir et al [ 2010 ]; Inoue et al [ 2003 ]; Inoue et al [ 2008 ]; Ma et al [ 1995 ]; Ma et al [ 1999 ]; Mori and Nishizawa [ 1987 ]; Nozoye et al [ 2014a ]; Nozoye et al [ 2014b ]). DMA is then secreted to the rhizosphere via the mugineic acid transporter (OsTOM1) Nozoye et al [ 2011 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of rice in response to iron deficiency and excess

Bashir

Hanada

Shimizu

et al. 2014

Rice

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BackgroundIron (Fe) is essential micronutrient for plants and its deficiency as well as toxicity is a serious agricultural problem. The mechanisms of Fe deficiency are reasonably understood, however our knowledge about plants response to excess Fe is limited. Moreover, the regulation of small open reading frames (sORFs) in response to abiotic stress has not been reported in rice. Understanding the regulation of rice transcriptome in response to Fe deficiency and excess could provide bases for developing strategies to breed plants tolerant to Fe deficiency as well as excess Fe.ResultsWe used a novel rice 110 K microarray harbouring ~48,620 sORFs to understand the transcriptomic changes that occur in response to Fe deficiency and excess. In roots, 36 genes were upregulated by excess Fe, of which three were sORFs. In contrast, 1509 genes were upregulated by Fe deficiency, of which 90 (6%) were sORFs. Co-expression analysis revealed that the expression of some sORFs was positively correlated with the genes upregulated by Fe deficiency. In shoots, 50 (19%) of the genes upregulated by Fe deficiency and 1076 out of 2480 (43%) genes upregulated by excess Fe were sORFs. These results suggest that excess Fe may significantly alter metabolism, particularly in shoots.ConclusionThese data not only reveal the genes regulated by excess Fe, but also suggest that sORFs might play an important role in the response of plants to Fe deficiency and excess.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-014-0018-1) contains supplementary material, which is available to authorized users.

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“…The subcellular compartmentalization of enzymes and transporters provides another layer of functional regulation. It was proposed that NA and DMA are synthesized in speci c vesicles, which may derive from ER [49,50]. In line with this model, OsNAS2 was found to localize in moving vesicles, depending on its tyrosine (YXXφ) and di-leucine (LL) motifs [51].…”

Section: Zmdmas1 Under Fe De Ciency the Accumulation Of Zmdmas-l2 Zmentioning

confidence: 70%

Genome-wide analysis of NAAT, DMAS, TOM, and ENA gene families in maize reveals their roles in regulating iron homeostasis

Zhang¹,

Zhou²,

Li³

et al. 2020

Preprint

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Background: Nicotianamine (NA) serves as not only the major chelator for iron transport but also the intermediate for synthesizing mugineic acid family phytosiderophores (MAs) which are secreted by graminaceous plants for Fe uptake. Therefore, the production and secretion of MAs are key steps for maintaining iron homeostasis in plants. Nicotianamine aminotransferase (NAAT), 2’-deoxymugineic acid synthase (DMAS), MAs efflux transporter (TOM), and efflux transporter of NA (ENA) were identified to be involved in these processes in rice and barley, whereas little systematic study has been performed in maize (Zea mays.L). Results: Here, we identified five ZmNAAT, nine ZmDMAS, eleven ZmTOM, and two ZmENA genes in maize by genome mining. RNA-sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of these genes exhibited diverse tissue specificity and different responses to environmental iron conditions. Moreover, the expression patterns were related to their evolution relationships. In particular, the ZmNAAT family can be classified into two subgroups, with one group showed inhibited expression in root under iron excess status and another subclass were repressed in shoot under both iron deficiency and excess. Likewise, the expression of ZmDMAS1 was stimulated under iron deficiency, while the remaining genes fell into two sub-clades with different expression patterns. Significant up-regulation of ZmTOM1, ZmTOM3 and ZmENA1 were observed under iron starvation, while ZmTOM2 was induced under both iron-excess and deficiency. These results reflect changing demands for the synthesis and secretion of NA/MAs to balance iron homeostasis under fluctuating conditions. All the examined ZmNAAT and ZmDMAS proteins localized in cytoplasm, while plasma and tonoplast membrane, endomembrane, and vesicle localization were observed for ZmTOM and ZmENA proteins. These results indicate that ZmTOM and ZmENA proteins may contribute to not only intercellular export but also intracellular sequestration of NA and MAs to facilitate iron homeostasis. Conclusions: Our results suggest that different gene expression profiles and subcellular localization of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA members may enable dedicate regulation of NA and phytosiderophores (PS) metabolism, shedding light on the understanding of iron-homeostasis in maize. Additionally, we also provided candidate genes for breeding iron-rich maize varieties.

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Rice nicotianamine synthase localizes to particular vesicles for proper function

Cited by 25 publications

References 42 publications

Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean

Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean

Transcriptomic analysis of rice in response to iron deficiency and excess

Genome-wide analysis of NAAT, DMAS, TOM, and ENA gene families in maize reveals their roles in regulating iron homeostasis

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