Rice OsERG3 encodes an unusual small C2-domain protein containing a Ca2+-binding module but lacking phospholipid-binding properties

Kang, Chang Ho; Moon, Byeong Cheol; Park, Hyeong Cheol; Koo, Seong Cheol; Jeon, Joo Mi; Cheong, Yong Hwa; Chung, Woo Sik; Lim, Chae Oh; Kim, Jae-Yeon; Yoon, Byung-Dae; Lee, Sang Yeol; Kim, Cha Young

doi:10.1016/j.bbagen.2011.06.021

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…ERG is a soluble cytosolic protein with a small C2 Ca 2+ -binding domain, which participates in the Ca 2+ signaling pathways in response to fungal effectors [ 84 ]. The transcriptional levels of the erg genes were dramatically upregulated in O. sativa L. treated with fungal elicitor obtained from mycelia of Magnaporthe grisea [ 85 ]. On the other hand, tpr genes have been shown to be involved in the transcriptional reprogramming of multiple defense genes in plants [ 86 ].…”

Section: Resultsmentioning

confidence: 99%

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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Anthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Physcomitrium patens Hedw. B&S (former Physcomitrella patens) is sensitive to known bacterial and fungal phytopathogens, including C. gloeosporioides, which cause infection and cell death. P. patens responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of P. patens and C. gloeosporioides have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of P. patens to C. gloeosporioides. Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 P. patens genes were differentially expressed in response to Colletotrichum infection. The analysis of differentially expressed gene function showed that the C. gloeosporioides infection led to a transcription reprogramming in P. patens that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, P. patens changes its transcription from primary metabolism to defend itself from the pathogen.

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Section: Resultsmentioning

confidence: 99%

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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“…To assess if gene expression contributed to the observed changes in the (phospho)proteome, 19 genes for proteins that may be involved in pathogenesis or defense processes obtained from both proteomic and phosphoproteomic analyses were selected for analysis. ERG3 (elicitor-responsive protein 3) was found to play a role in plant defense signaling, where ERG3 transcript levels were greatly enhanced by treatment of rice with a fungal elicitor and a Ca 2+ -ionophore [ 30 ]. BIP (binding immunoglobulin protein, heat shock 70 kDa protein) and PR2B (glucan endo-1,3-β-glucosidase) belong to the stress response-associated proteins [ 3 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Wrzesińska

Gevaert

et al. 2018

IJMS

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Signaling in host plants is an integral part of a successful infection by pathogenic RNA viruses. Therefore, identifying early signaling events in host plants that play an important role in establishing the infection process will help our understanding of the disease process. In this context, phosphorylation constitutes one of the most important post-translational protein modifications, regulating many cellular signaling processes. In this study, we aimed to identify the processes affected by infection with Peanut stunt virus (PSV) and its satellite RNA (satRNA) in Nicotiana benthamiana at the early stage of pathogenesis. To achieve this, we performed proteome and phosphoproteome analyses on plants treated with PSV and its satRNA. The analysis of the number of differentially phosphorylated proteins showed strong down-regulation in phosphorylation in virus-treated plants (without satRNA). Moreover, proteome analysis revealed more down-regulated proteins in PSV and satRNA-treated plants, which indicated a complex dependence between proteins and their modifications. Apart from changes in photosynthesis and carbon metabolism, which are usually observed in virus-infected plants, alterations in proteins involved in RNA synthesis, transport, and turnover were observed. As a whole, this is the first community (phospho)proteome resource upon infection of N. benthamiana with a cucumovirus and its satRNA and this resource constitutes a valuable data set for future studies.

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“…ERG3 (elicitor-responsive protein 3) encodes a small C2-domain protein, which is a common Ca 2+ -dependent lipid-binding motif that is abundantly present in membrane-associated proteins involved in both signaling and membrane trafficking [ 68 ]. Most small C2-domain proteins have been shown to be involved in plant defenses or stress responses [ 69 ]. Furthermore, KAS3A (3-oxoacyl-[acyl-carrier-protein] synthase 3 A) is involved in the regulation of aliphatic acid biosynthesis by catalyzing the condensation of acetyl-COA with a malonyl-acyl carrier protein in dissociated (type 11) fatty-acid synthase systems.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis of 101 accessions dissects the genetic basis of shell thickness for genetic improvement in Persian walnut (Juglans regia L.)

Wang

Zhou

et al. 2022

BMC Plant Biol

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Background Understanding the underlying genetic mechanisms that drive phenotypic variations is essential for enhancing the efficacy of crop improvement. Persian walnut (Juglans regia L.), which is grown extensively worldwide, is an important economic tree fruit due to its horticultural, medicinal, and material value. The quality of the walnut fruit is related to the selection of traits such as thinner shells, larger filling rates, and better taste, which is very important for breeding in China. The complex quantitative fruit-related traits are influenced by a variety of physiological and environmental factors, which can vary widely between walnut genotypes. Results For this study, a set of 101 Persian walnut accessions were re-sequenced, which generated a total of 906.2 Gb of Illumina sequence data with an average read depth of 13.8× for each accession. We performed the genome-wide association study (GWAS) using 10.9 Mb of high-quality single-nucleotide polymorphisms (SNPs) and 10 agronomic traits to explore the underlying genetic basis of the walnut fruit. Several candidate genes are proposed to be involved in walnut characteristics, including JrPXC1, JrWAKL8, JrGAMYB, and JrFRK1. Specifically, the JrPXC1 gene was confirmed to participate in the regulation of secondary wall cellulose thickening in the walnut shell. Conclusion In addition to providing considerable available genetic resources for walnut trees, this study revealed the underlying genetic basis involved in important walnut agronomic traits, particularly shell thickness, as well as providing clues for the improvement of genetic breeding and domestication in other perennial economic crops.

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Rice OsERG3 encodes an unusual small C2-domain protein containing a Ca2+-binding module but lacking phospholipid-binding properties

Cited by 5 publications

References 21 publications

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Genome-wide association analysis of 101 accessions dissects the genetic basis of shell thickness for genetic improvement in Persian walnut (Juglans regia L.)

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