Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter

Yang, Won Tae; Baek, Dongwon; Yun, Dae‐Jin; Lee, Kwang-Sik; Hong, So Yeon; Bae, Ki Deuk; Chung, Young Soo; Kwon, Yong Sham; Kim, Du Hyun; Jung, Ki‐Hong; Kim, Doh Hoon

doi:10.1371/journal.pone.0194628

Cited by 33 publications

(34 citation statements)

References 66 publications

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“…The EMSA was performed using the Lightshift Chemiluminescent EMSA kit (Thermo Fisher Scientific) according to the manufacturer's instructions (Yang et al, 2018). The probes were labeled with the 39 end biotin (Cosmo Genetech), oligonucleotides spanning the ABRE binding site motif on the NCED3 promoter.…”

Section: Emsamentioning

confidence: 99%

The GIGANTEA-ENHANCED EM LEVEL Complex Enhances Drought Tolerance via Regulation of Abscisic Acid Synthesis

et al. 2020

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Drought is one of the most critical environmental stresses limiting plant growth and crop productivity. The synthesis and signaling of abscisic acid (ABA), a key phytohormone in the drought stress response, is under photoperiodic control. GIGANTEA (GI), a key regulator of photoperiod-dependent flowering and the circadian rhythm, is also involved in the signaling pathways for various abiotic stresses. In this study, we isolated ENHANCED EM LEVEL (EEL)/basic Leu zipper 12, a transcription factor involved in ABA signal responses, as a GI interactor in Arabidopsis (Arabidopsis thaliana). The diurnal expression of 9-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), a rate-limiting ABA biosynthetic enzyme, was reduced in the eel, gi-1, and eel gi-1 mutants under normal growth conditions. Chromatin immunoprecipitation and electrophoretic mobility shift assays revealed that EEL and GI bind directly to the ABA-responsive element motif in the NCED3 promoter. Furthermore, the eel, gi-1, and eel gi-1 mutants were hypersensitive to drought stress due to uncontrolled water loss. The transcript of NCED3, endogenous ABA levels, and stomatal closure were all reduced in the eel, gi-1, and eel gi-1 mutants under drought stress. Our results suggest that the EEL-GI complex positively regulates diurnal ABA synthesis by affecting the expression of NCED3, and contributes to the drought tolerance of Arabidopsis.

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Section: Emsamentioning

confidence: 99%

The GIGANTEA-ENHANCED EM LEVEL Complex Enhances Drought Tolerance via Regulation of Abscisic Acid Synthesis

et al. 2020

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“…Amongst these OsPTs, OsPT1 is thoroughly stated in roots and is a main regulator of Pi. OsPT2 has a vital role in Pi transferring from the root to the shoot [58]. AsV may be reduced to AsIII inside the rice root, and AsIII may then enter the xylem via a silicic acid/AsIII effluxer [59].…”

Section: Arsenic Species Translocation From Root To Shootmentioning

confidence: 99%

Arsenic Uptake and Accumulation Mechanisms in Rice Species

Abedi

Mojiri

2020

Plants

100

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Rice consumption is a source of arsenic (As) exposure, which poses serious health risks. In this study, the accumulation of As in rice was studied. Research shows that As accumulation in rice in Taiwan and Bangladesh is higher than that in other countries. In addition, the critical factors influencing the uptake of As into rice crops are defined. Furthermore, determining the feasibility of using effective ways to reduce the accumulation of As in rice was studied. AsV and AsIII are transported to the root through phosphate transporters and nodulin 26-like intrinsic channels. The silicic acid transporter may have a vital role in the entry of methylated As, dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA), into the root. Amongst As species, DMA(V) is particularly mobile in plants and can easily transfer from root to shoot. The OsPTR7 gene has a key role in moving DMA in the xylem or phloem. Soil properties can affect the uptake of As by plants. An increase in organic matter and in the concentrations of sulphur, iron, and manganese reduces the uptake of As by plants. Amongst the agronomic strategies in diminishing the uptake and accumulation of As in rice, using microalgae and bacteria is the most efficient.

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“…The MYB family transcription factors PHOSPHATE STARVATION RESPONSE REGULATOR 1 (PHR1) and PHL1–4 (PHR1‐LIKE 1–4) in Arabidopsis and their rice ( Oryza sativa ) homologs OsPHR1–4 are important components in Pi starvation signaling and regulate a subset of Pi starvation‐induced (PSI) genes (Rubio et al ., 2001; Bustos et al ., 2010; Chiou and Lin, 2011; Guo et al ., 2015; Sun et al ., 2016; Wang et al ., 2018). The MYB transcription factors OsMYB2P‐1, OsMYB4P, and OsMYB5P are also involved in regulating the Pi starvation response, including the expression of several P transporter (PT)‐encoding genes (Dai et al ., 2012; Yang et al ., 2018). In addition, the WRKY family transcription factors AtWRKY6, 42, 45, and 75 in Arabidopsis and OsWRKY74 in rice regulate PSI gene expression (Devaiah et al ., 2007; Chen et al ., 2009; Wang et al ., 2014a; Su et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

OsbHLH6 interacts with OsSPX4 and regulates the phosphate starvation response in rice

Lü

Guo

et al. 2020

The Plant Journal

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SUMMARY Low soil phosphorus (P) availability is a major limitation for crop production. The molecular mechanisms underlying plant responses and adaptation to phosphate (Pi) deficiency are unclear. OsbHLH6 (hereafter bHLH6), an uncharacterized rice (Oryza sativa) Pi starvation response gene encoding a basic helix‐loop‐helix protein, was identified by yeast two‐hybrid screening using the phosphate response repressor OsSPX4 (hereafter SPX4) as bait. bHLH6 is expressed in shoots and roots, and its expression is significantly induced in shoots by Pi deficiency. bHLH6 overexpression lines showed Pi accumulation and enhanced Pi starvation responses, including upregulation of Pi starvation‐induced genes and longer root hairs. A bhlh6 mutant showed no significant phenotype variation at the seedling stage. A pull‐down assay indicated that bHLH6 had higher binding affinity with SPX4 compared to OsPHR2; therefore, bHLH6 competitively inhibited the interaction of SPX4 and OsPHR2. SPX4 overexpression rescued the Pi accumulation caused by bHLH6 overexpression under high‐ and low‐P conditions. Moreover, overexpression of bHLH6 in an spx4 background did not affect the Pi content of spx4 under high‐ and low‐P conditions. The bhlh6 spx4 double mutant showed lower shoot Pi concentrations and transcript levels of OsPT3 and OsPT10 compared with the spx4 mutant under high‐P conditions. RNA sequencing results indicated that bHLH6 overexpression and spx4 mutant lines share many differentially expressed Pi‐responsive genes. Therefore, bHLH6 is an important regulator for Pi signaling and homeostasis which antagonizes SPX4. This knowledge helps elucidate the molecular regulation of plant adaptation to Pi deficiency and will promote efforts toward the creation of low Pi‐tolerant crops.

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Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter

Cited by 33 publications

References 66 publications

The GIGANTEA-ENHANCED EM LEVEL Complex Enhances Drought Tolerance via Regulation of Abscisic Acid Synthesis

The GIGANTEA-ENHANCED EM LEVEL Complex Enhances Drought Tolerance via Regulation of Abscisic Acid Synthesis

Arsenic Uptake and Accumulation Mechanisms in Rice Species

OsbHLH6 interacts with OsSPX4 and regulates the phosphate starvation response in rice

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