RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

Abstract

The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL -1 (259.1 μg g -1 ) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2 IV 6-2 fractional factorial design sequenced by a 2 4 central composite design). The conditions for maximizing the production of carotenoids were (g L -1) malt extract (16.25), peptone (8.75), sucrose (15) and rice parboiling wastewater (87.5), with a pH of 5 at 25 °C and 150 rpm for 144 h, which produced a concentration 5.3 μg mL -1 (628.8 μg g -1 ).Index terms: Biodye; submerged cultive; response surface methodology; yeast. RESUMOO interesse pela produção de carotenoides por fontes naturais é crescente, em decorrência da possibilidade de atuar como corante e sua capacidade antioxidante biológica potente. Neste estudo, avaliou-se o uso da água de parboilização do arroz, como substrato alternativo para a bioprodução de carotenoides, usando a levedura Phaffia rhodozyma que, como única fonte de nutrientes, foi promissora, alcançando 0,6 µg mL -1 (259,1 µg g -1

Microencapsulation by lyophilization of carotenoids produced by Phaffia rhodozyma with soy protein as the encapsulating agent

“…It was incubated at 25 °C, 150 rpm for 48 h or the period of time needed to reach 1×10 8 cel.mL , counted by the Neubauer chamber (Rios et al, 2015). Bioproduction of carotenoids was carried out in 500 mL Erlenmeyer flasks with 153 mL YM production medium, at initial pH of 6.0, and 10% inoculum (10 8 cel.mL -1…”

“…The operating conditions of the process were 25 °C, 180 rpm for 168 h (Rios et al, 2015). The resulting biomasses were dried in an oven at 35 °C for 24 h. Then, they…”

“…By comparison with other microorganisms and other natural sources, it has advantages, such as the possibility of using alternative inexpensive media, since it requires simple sources of carbon and nitrogen for its growth and little physical space when it comes from crops on an industrial scale, besides being independent of bioproducts seasonality (Cipolatti et al, 2015;Valduga et al, 2009b).…”

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

“…Rios et al (16) observed positive eff ects of the increase in malt extract concentration on carotenoid production but not of the yeast extract concentration when using P. rhodozyma in similar culture media without the use of raw glycerol. The yeast extract and other nutrients added to the culture medium did not infl uence the production of carotenoids by Xanthophyllomyces dendrorhous ENM 5 at 20 °C and 250 rpm.…”

“…In a previous study carried out by our research group, the production of carotenoids by the wild yeast Sporidiobolus pararoseus with the use of a complex medium containing by-products such as raw glycerol, corn steep liquor and sugar cane molasses was investigated (15). The production of carotenoids from Phaffi a rhodozyma NRRL Y-17268 was investigated in a culture medium containing pure glycerol and raw glycerol as additional carbon sources (8) and parboiled rice effl uent as nitrogen and phosphorus sources (16). Moreover, carotenoids produced by this yeast strain had antioxidant activity (expressed as Trolox equivalent) of 4.00 and 3.66 mM/μg in ABTS radical cation discoloration and ferric reducing antioxidant power assays, respectively (17).…”