Rice volatiles lure gravid malaria mosquitoes, Anopheles arabiensis

Wondwosen, Betelehem; Birgersson, Göran; Seyoum, Emiru; Tekie, Habte; Torto, Baldwyn; Fillinger, Ulrike; Hill, Sharon R.; Ignell, Rickard

doi:10.1038/srep37930

Cited by 74 publications

(119 citation statements)

References 53 publications

Supporting

Mentioning

117

Contrasting

Order By: Relevance

“…arabiensis . Three components, limonene, α -pinene and nonanal, are shared between the synthetic maize pollen blend and the eight-component blend identified from the headspace of the MR3 rice cultivar [17]. For both blends, the full component blends are required to elicit the full behavioural repertoire of the gravid mosquitoes, which is in line with studies on herbivorous insects showing that blend perception is critical for host plant recognition and behavioural responses [18].…”

Section: Discussionmentioning

confidence: 59%

“…The five-component blend identified from BH-660 maize pollen constitutes the second synthetic blend identified from a cereal crop [17] shown to attract and stimulate oviposition in gravid An. arabiensis .…”

Section: Discussionmentioning

confidence: 99%

“…A two-port olfactometer [17] was used to test the attraction preference of the mosquitoes for the headspace volatiles collected from the BH-660 and ZM-521 pollen, and from natural breeding water. All assays were conducted between 18:00 and 21:00, which is the peak period of oviposition activity as determined in pilot experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The oviposition preference of gravid mosquitoes was analysed in a two-choice assay [17]. Metal wire framed cages (30 cm × 30 cm × 30 cm) covered with white nylon mosquito netting were used.…”

Section: Methodsmentioning

confidence: 99%

“…Although some of the compounds released by these sources have been identified for Aedes and Culex mosquitoes [2, 3], we are only now starting to understand the complexity of chemical cues regulating oviposition site selection in Anopheles mosquitoes [16, 17]. To date, synthetic compounds that attract and stimulate oviposition in gravid Anopheles gambiae and Anopheles arabiensis have been identified from rice [17], as well as microbes associated with their larval habitats [16]. The aim of this study was to characterize the behavioural response of gravid An.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A(maize)ing attraction: gravid Anopheles arabiensis are attracted and oviposit in response to maize pollen odours

Wondwosen

Hill

Birgersson

et al. 2017

Malar J

Self Cite

View full text Add to dashboard Cite

BackgroundMaize cultivation contributes to the prevalence of malaria mosquitoes and exacerbates malaria transmission in sub-Saharan Africa. The pollen from maize serves as an important larval food source for Anopheles mosquitoes, and females that are able to detect breeding sites where maize pollen is abundant may provide their offspring with selective advantages. Anopheles mosquitoes are hypothesized to locate, discriminate among, and select such sites using olfactory cues, and that synthetic volatile blends can mimic these olfactory-guided behaviours.MethodsTwo-port olfactometer and two-choice oviposition assays were used to assess the attraction and oviposition preference of gravid Anopheles arabiensis to the headspace of the pollen from two maize cultivars (BH-660 and ZM-521). Bioactive compounds were identified using combined gas chromatography and electroantennographic detection from the headspace of the cultivar found to be most attractive (BH-660). Synthetic blends of the volatile compounds were then assessed for attraction and oviposition preference of gravid An. arabiensis, as above.ResultsHere the collected headspace volatiles from the pollen of two maize cultivars was shown to differentially attract and stimulate oviposition in gravid An. arabiensis. Furthermore, a five-component synthetic maize pollen odour blend was identified, which elicited the full oviposition behavioural repertoire of the gravid mosquitoes.ConclusionsThe cues identified from maize pollen provide important substrates for the development of novel control measures that modulate gravid female behaviour. Such measures are irrespective of indoor or outdoor feeding and resting patterns, thus providing a much-needed addition to the arsenal of tools that currently target indoor biting mosquitoes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-016-1656-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A(maize)ing attraction: gravid Anopheles arabiensis are attracted and oviposit in response to maize pollen odours

Wondwosen

Hill

Birgersson

et al. 2017

Malar J

Self Cite

View full text Add to dashboard Cite

show abstract

Multiband modulation spectroscopy for the determination of sex and species of mosquitoes in flight

Gebru

Jansson

Ignell

et al. 2018

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

We present a dual-wavelength polarimetric measurement method to distinguish species and sexes of disease transmitting mosquitoes in flight. By measuring co- and de-polarized backscattered light at 808 and 1550 nm, the degree of linear polarization, wingbeat frequency, reflectance, spectral ratio and glossiness of mosquitoes can be retrieved. Body and wing contributions to these signals can be separated. Whereas the optical cross section is sensitive to the aspect of observation, thus the heading direction of the insect in flight, we demonstrate that polarimetric- and spectral-band ratios are largely invariant to the aspect of observation. We show that wing glossiness, as well as wing- and body-spectral ratios are particularly efficient in distinguishing Anopheles coluzzii and Anopheles arabiensis, 2 closely related species of malaria vectors. Spectral and polarimetric ratios relate to microstructural and melanization features of the wing and body of these species. We conclude that multiband modulation spectroscopy is a useful expansion of the parameter space that can be used to improve the specificity of entomological lidars.

show abstract

Modulation of odour-guided behaviour in mosquitoes

Hill

2021

Cell Tissue Res

Self Cite

View full text Add to dashboard Cite

Mosquitoes are emerging as model systems with which to study innate behaviours through neuroethology and functional genomics. Decades of work on these disease vectors have provided a solid behavioural framework describing the distinct repertoire of predominantly odour-mediated behaviours of female mosquitoes, and their dependence on life stage (intrinsic factors) and environmental cues (extrinsic factors). The purpose of this review is to provide an overview of how intrinsic factors, including adult maturation, age, nutritional status, and infection, affect the attraction to plants and feeding on plant fluids, host seeking, blood feeding, supplemental feeding behaviours, pre-oviposition behaviour, and oviposition in female mosquitoes. With the technological advancements in the recent two decades, we have gained a better understanding of which volatile organic compounds are used by mosquitoes to recognise and discriminate among various fitness-enhancing resources, and characterised their neural and molecular correlates. In this review, we present the state of the art of the peripheral olfactory system as described by the neural physiology, functional genomics, and genetics underlying the demonstrated changes in the behavioural repertoire in female mosquitoes. The review is meant as a summary introduction to the current conceptual thinking in the field.

show abstract

Rice volatiles lure gravid malaria mosquitoes, Anopheles arabiensis

Cited by 74 publications

References 53 publications

A(maize)ing attraction: gravid Anopheles arabiensis are attracted and oviposit in response to maize pollen odours

A(maize)ing attraction: gravid Anopheles arabiensis are attracted and oviposit in response to maize pollen odours

Multiband modulation spectroscopy for the determination of sex and species of mosquitoes in flight

Modulation of odour-guided behaviour in mosquitoes

Contact Info

Product

Resources

About