Rich variant phenotype of Gossypium hirsutum L. saturated mutant library provides resources for cotton functional genomics and breeding

Wei, Yunxiao; Liu, Yongming; Ali, Abid Muhammad; Xiao, Rong; Liang, Chengzhen; Meng, Zhen; Wang, Yuan; Wang, Peilin; Wang, Xingfen; Zhang, Rui

doi:10.1016/j.indcrop.2022.115232

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…Functional genomics has emerged as a crucial scientific discipline in recent years, revolutionizing our understanding of plant biology and genetics improvement ( Van Bel et al, 2022 ). In the case of cotton, functional genomics offers the potential to systematically exploit genetic resources, improve resistance against biotic stresses, enhance yield, and optimize germplasm for cultivation ( Chen et al, 2007 ; Wei et al, 2022 ). However, several challenges persist in cotton functional genomics, including the long growth cycle, low transformation efficiency, large genome size and the lack of efficient molecular tools.…”

Section: Next-generation Sequencing Applications In Cotton Genomicsmentioning

confidence: 99%

A comprehensive review on Gossypium hirsutum resistance against cotton leaf curl virus

Nadeem,

Riaz Ahmed,

Luqman

et al. 2024

Front. Genet.

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Cotton (Gossypium hirsutum L.) is a significant fiber crop. Being a major contributor to the textile industry requires continuous care and attention. Cotton is subjected to various biotic and abiotic constraints. Among these, biotic factors including cotton leaf curl virus (CLCuV) are dominant. CLCuV is a notorious disease of cotton and is acquired, carried, and transmitted by the whitefly (Bemisia tabaci). A cotton plant affected with CLCuV may show a wide range of symptoms such as yellowing of leaves, thickening of veins, upward or downward curling, formation of enations, and stunted growth. Though there are many efforts to protect the crop from CLCuV, long-term results are not yet obtained as CLCuV strains are capable of mutating and overcoming plant resistance. However, systemic-induced resistance using a gene-based approach remained effective until new virulent strains of CLCuV (like Cotton Leaf Curl Burewala Virus and others) came into existence. Disease control by biological means and the development of CLCuV-resistant cotton varieties are in progress. In this review, we first discussed in detail the evolution of cotton and CLCuV strains, the transmission mechanism of CLCuV, the genetic architecture of CLCuV vectors, and the use of pathogen and nonpathogen-based approaches to control CLCuD. Next, we delineate the uses of cutting-edge technologies like genome editing (with a special focus on CRISPR-Cas), next-generation technologies, and their application in cotton genomics and speed breeding to develop CLCuD resistant cotton germplasm in a short time. Finally, we delve into the current obstacles related to cotton genome editing and explore forthcoming pathways for enhancing precision in genome editing through the utilization of advanced genome editing technologies. These endeavors aim to enhance cotton’s resilience against CLCuD.

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Section: Next-generation Sequencing Applications In Cotton Genomicsmentioning

confidence: 99%

A comprehensive review on Gossypium hirsutum resistance against cotton leaf curl virus

Nadeem,

Riaz Ahmed,

Luqman

et al. 2024

Front. Genet.

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“…In the realm of cotton research, notable advancements have been achieved in the development of EMS-induced mutant libraries within diverse cotton species, such as G. arboreum ( Kong et al., 2017 ), G. barbadense ( Abid et al., 2020 ), G. hirsutum ( Lian et al., 2020 ; Wei et al., 2022 ), and G. herbaceum ( Kumar et al., 2022 ). It is noteworthy that the primary focus of these events centered on conventional cultivated G. hirsutum varieties.…”

Section: Ems-induced Mutant Libraries In Cottonmentioning

confidence: 99%

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Wang,

Abbas,

et al. 2024

Front. Plant Sci.

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Cotton production faces challenges in fluctuating environmental conditions due to limited genetic variation in cultivated cotton species. To enhance the genetic diversity crucial for this primary fiber crop, it is essential to augment current germplasm resources. High-throughput sequencing has significantly impacted cotton functional genomics, enabling the creation of diverse mutant libraries and the identification of mutant functional genes and new germplasm resources. Artificial mutation, established through physical or chemical methods, stands as a highly efficient strategy to enrich cotton germplasm resources, yielding stable and high-quality raw materials. In this paper, we discuss the good foundation laid by high-throughput sequencing of cotton genome for mutant identification and functional genome, and focus on the construction methods of mutant libraries and diverse sequencing strategies based on mutants. In addition, the important functional genes identified by the cotton mutant library have greatly enriched the germplasm resources and promoted the development of functional genomes. Finally, an innovative strategy for constructing a cotton CRISPR mutant library was proposed, and the possibility of high-throughput screening of cotton mutants based on a UAV phenotyping platform was discussed. The aim of this review was to expand cotton germplasm resources, mine functional genes, and develop adaptable materials in a variety of complex environments.

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“…Among various mutagenic methods, chemical reagents that can produce genetic variation in crops have been widely popular for many years. A mutation strategy is considered to be an important step for producing new and valuable genetic variations (Wei et al, 2022. Many important crops, such as wheat, sesame, rice, grapefruit, cotton, and bananas, have produced mutant-derived varieties. In tomato, EMS-based mutations have been used to generate specific alleles such as a purple leaf color, smaller leaves, and early fruit (Siluéet al, 2013).…”

Section: Recent Studies In Plants Using Ems Mutagenesismentioning

confidence: 99%

Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development

Chen

Liu²,

Sun³

et al. 2023

Front. Plant Sci.

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Ethyl methanesulfonate (EMS)-induced mutagenesis is a powerful tool to generate genetic resource for identifying untapped genes and characterizing the function of genes to understand the molecular basis of important agronomic traits. This review focuses on application of contemporary EMS mutagenesis in the field of plant development and abiotic stress tolerance research, with particular focuses on reviewing the mutation types, mutagenesis site, mutagen concentration, mutagenesis duration, the identification and characterization of mutations responsible for altered stress tolerance responses. The application of EMS mutation breeding combined with genetic engineering in the future plant breeding and fundamental research was also discussed. The collective information in this review will provide good insight on how EMS mutagenesis is efficiently applied to improve abiotic stress tolerance of crops with the utilization of Next-generation sequencing (NGS) for mutation identification.

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Rich variant phenotype of Gossypium hirsutum L. saturated mutant library provides resources for cotton functional genomics and breeding

Cited by 9 publications

References 49 publications

A comprehensive review on Gossypium hirsutum resistance against cotton leaf curl virus

A comprehensive review on Gossypium hirsutum resistance against cotton leaf curl virus

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development

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