Ridge augmentation using recombinant human fibroblast growth factor‐2 with biodegradable gelatin sponges incorporating β‐tricalcium phosphate: a preclinical study in dogs

Abstract

The combined use of rhFGF-2 and gelatin/β-TCP sponge promotes ridge augmentation in canine saddle-type bone defects.

Comparison between different isoelectric points of biodegradable gelatin sponges incorporating β‐tricalcium phosphate and recombinant human fibroblast growth factor‐2 for ridge augmentation: A preclinical study of saddle‐type defects in dogs

“…Compared to our previous study conducted under similar conditions, the amount of bone augmentation in the acidic group in the present study (total tissue height: 7.11 ± 0.69 mm; new bone height: 3.72 ± 0.58 mm) twelve weeks after operation was similar to the amount observed eight weeks after operation (total tissue height: 6.62 ± 0.66 mm; new bone height: 2.98 ± 0.65 mm). In the present study, bone height in the basic group (total tissue height: 6.63 ± 0.87 mm; new bone height: 2.88 ± 0.71 mm) was slightly higher than that in our previous study using acidic gelatin/β‐TCP sponges (IEP 5.0) with normal saline (total tissue height: 5.95 ± 0.74 mm; new bone height: 1.56 ± 0.66 mm) . These results indicate that the effectiveness of rhFGF‐2 release from basic gelatin/β‐TCP sponges (IEP 9.0) is detectable, albeit small.…”

“…In vitro studies showed that acidic gelatin sponge (IEP 5.0) had a high affinity to rhFGF‐2, which is basic in vivo, and formed gelatin complexes ionically . The effect of controlled rhFGF‐2 release can be achieved by gelatin decomposition to enhance hard and soft tissue regeneration in in vivo studies . On the contrary, in vitro studies have reported that basic gelatin sponges (IEP 9.0) rarely have an affinity to rhFGF‐2 and do not exhibit polyion complexation.…”

“…Saddle‐type bone defects (8 mm length × 4 mm depth) were surgically created bilaterally using a steel bar 2 mm mesial from the maxillary canine mesial site, removing all buccal‐palatal alveolar ridges between them. The average buccal‐lingual width was approximately 10 mm (Figure B) . Gelatin/β‐TCP sponges were trimmed to match the size of the defects (8 mm length × 4 mm depth × 10 mm width) using a surgical knife.…”

“…Histological measurement was based on a previously reported method with a slight modification . Briefly, after being trimmed, the specimens were decalcified for approximately 1 month in Plank‐Rychlo's solution, dehydrated, and embedded in paraffin (HISTPREP 568, Wako Pure Chemical Industries Ltd., Osaka, Japan) and cut into 6‐μm‐thick sections on the sagittal plane.…”

“…Light microscopy (Eclipse Ni‐U, Nikon Corporation, Tokyo, Japan; DP70, Olympus Corporation, Tokyo, Japan) was used for histological observations and analysis using a computerized image system consisting of a high‐definition color camera head (DS‐Fi2; Nikon Corporation) and a microscope camera control unit (DS‐L3; Nikon Corporation) equipped with a measurement function. According to a previous study, new bone height (defined as the distance between the top of the new bone and the line connecting buccal and lingual tops of the host bone) and total tissue height (defined as the distance between the top of the soft tissue and the line connecting the buccal and lingual tops of the host bone) were calculated . To minimize intra‐examiner errors, two blinded, experienced examiners (T.A.…”