Riluzole-loaded lipid nanoparticles for brain delivery: Preparation, optimization and characterization

Teixeira, Maria Inês; Lopes, Carla Martins; Gonçalves, Hugo; Catita, José; Silva, Ana Margarida; Rodrigues, Francisca; Amaral, Maria Helena; Costa, Paulo

doi:10.1016/j.molliq.2023.122749

Cited by 8 publications

(2 citation statements)

References 92 publications

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“…However, DNase-CS-N displayed a larger average particle size of 427.0 ± 15.1 nm, possibly due to the conjugation of DNase I onto the surface of CS-N. The PDI of CSNPs, CS-N, and DNase-CS-N were all lower than 0.3, which indicates that the NPs produced in this study have uniform particle size distribution and better dispersion (Teixeira et al, 2023). The zeta potential of CSNPs, CS-N, and DNase-CS-N was 28.4 ± 2.5, 39.3 ± 3.7, and 52.5 ± 0.2 mV, respectively, which all have high zeta potential.…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 75%

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Hu,

Du,

et al. 2024

Journal of Food Science

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Listeria monocytogenes biofilms represent a continuous source of contamination, leading to serious food safety concerns and economic losses. This study aims to develop novel nisin‐loaded chitosan nanoparticles (CSNPs) functionalized with DNase I and evaluate its antibiofilm activity against L. monocytogenes on food contact surfaces. Nisin‐loaded CSNPs (CS‐N) were first prepared by ionic cross‐linking, and DNase I was covalently grafted on the surface (DNase‐CS‐N). The NPs were subsequently characterized by Zetasizer Nano, transmission electron microscopy, Fourier transform infrared (FT‐IR), and X‐ray diffraction (XRD). The antibiofilm activity of NPs was evaluated against L. monocytogenes on polyurethane (PU). The DNase‐CS‐N was fabricated and characterized with quality attributes (particle size—427.0 ± 15.1 nm, polydispersity [PDI]—0.114 ± 0.034, zeta potential—+52.5 ± 0.2 mV, encapsulation efficiency—46.5% ± 3.6%, DNase conjugate rate—70.4% ± 0.2). FT‐IR and XRD verified the loading of nisin and binding of DNase I with chitosan. The DNase‐CS‐N caused a 3 log colony‐forming unit (CFU)/cm2 reduction of L. monocytogenes biofilm cells, significantly higher than those in CSNPs (1.4 log), CS‐N (1.8 log), and CS‐N in combination with DNase I (2.2 log) treatment groups. In conclusion, nisin‐loaded CSNPs functionalized with DNase I were successfully prepared and characterized with smooth surface and nearly spherical shape, high surface positive charge, and good stability, which is effective to eradicate L. monocytogenes biofilm cells on food contact surfaces, exhibiting great potential as antibiofilm agents in food industry.Practical ApplicationListeria monocytogenes biofilms are a common safety hazard in food processing. In this study, novel nanoparticles were successfully constructed and are expected to be a promising antibiofilm agent in the food industry.

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Section: Characterization Of Nanoparticlesmentioning

confidence: 75%

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Hu,

Du,

et al. 2024

Journal of Food Science

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“…Teixeira et al performed a Precirol ATO 5 thermogram, they revealed peak was observed at 67.8°C, whereas in physical mixture, curcumin exhibited a peak at 176.60°C, and Precirol ATO 5 at 68°C. The lyophilized Curcumin loaded NLC thermogram exhibited a sole melting peak at approximately 65°C, indicating a left shift in comparison to the Precirol ATO 5peak[31].This shift suggests a decrease in melting points, indicating a significant movement towards lower temperatures compared to solid lipid. This alteration hinted at the formation of irregular structures within the NLC.…”

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confidence: 94%

Design and development of gastro-retentive gel forming system comprising curcumin loaded Nanostructured lipid carrier for stomach specific delivery

Dhasaiyan,

Karuppaiah,

Rahman

2024

Preprint

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This study delved on development of curcumin loaded Nanostructured Lipid carrier (NLC) incorporated into gastro-retentive oral in situ gel for specifically designed to the stomach delivery. Curcumin is less soluble in stomach pH, so a high amount of drug would be required to produce the desired effects. The solubility of curcumin was improved by the NLC. The curcumin loaded NLC was fabricated using a high shear homogenization technique. Through lipid screening, Precirol ATO 5 and Labrafac PG were chosen as a lipids, and tween 80 was selected as a surfactant in this formulation. A formulation (NLC-F4) comprising 2% lipids and 800 mg of surfactant was chosen as the best formulation, average mean particle size was found to be 119.3 ± 0.13 nm, PDI was found to be 0.127 -30 ± 0.17 mV 0.02, zeta potential was found to be -30 ± 0.17 mV and % of encapsulation efficiency were found to be83.4 ± 1.5%. FT-IR investigation showed no interactions. Scanning Electron Microscopy (SEM) revealed smooth and spherical shaped particles, while Differential Scanning Calorimetry (DSC) assessment suggested that curcumin was completely distributed in the NLC. The in vitro drug release of curcumin from NLC-F4 showed 8 times enhanced drug release compared with the pure curcumin in pH 1.2 buffer. In vitro antioxidant and anti-inflammatory studies revealed significant effects compared to reference standards. Drug release kinetics followed first order release and Weibull kinetics models. The curcumin loaded NLC was fabricated into anoral in situ gel to enhances the gastric retention time and prolong release of drug in the stomach. The system exhibited quick gelation time, optimal viscosity, gastric buoyant density, 24 hours floating time, gastric peristalsis resistant gel strength, and sustained drug release. These investigations revealed that an oral in situ gelling system containing curcumin loaded NLC has potential as a carrier for stomach specific delivery and could serve as an alternative dosage form compared to conventional ones for treating gastric ulcers in patients.

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Design and Development of Gastro-retentive Gel Forming System Comprising Curcumin Loaded Nanostructured Lipid Carrier for Stomach Specific Delivery

Dhasaiyan,

Karuppaiah,

Rahman

2024

J Clust Sci

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Riluzole-loaded lipid nanoparticles for brain delivery: Preparation, optimization and characterization

Cited by 8 publications

References 92 publications

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Design and development of gastro-retentive gel forming system comprising curcumin loaded Nanostructured lipid carrier for stomach specific delivery

Design and Development of Gastro-retentive Gel Forming System Comprising Curcumin Loaded Nanostructured Lipid Carrier for Stomach Specific Delivery

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