Ring -opening of polythiolactones to construct protein-imprinted nanospheres with high recognition and regulation capabilities

Wang, Mingqi; Zhang, Guoxian; Liu, Qing; Wei, Mengmeng; Ren, Yafeng; Fa, Shixin; Zhang, Qiuyu

doi:10.1039/d2nr03715g

Cited by 3 publications

(2 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disappearance of the peak at 918 cm −1 in MS@PTL-g-PNIPAM provides strong evidence for the complete ammonolysis of the thiolactone side groups. [21] The characteristic element peaks of different nanospheres in the X-ray photoelectron spectra (XPS) also indicates that all modifications were in order and all nanospheres were prepared as expected (Figure 1d and Figure S12, Supporting Information). Furthermore, all nanospheres are thermally stable up to 200 °C (Figure S13, Supporting Information) and magnetic enough to meet the needs of magnetic separation (Figure S14, Supporting Information).…”

Section: Synthesis and Characterization Of Ms@ptl-g-pnipamsupporting

confidence: 58%

“…Next, three selected amine ligands (i.e., ethanolamine, β‐alanine, 1‐(2‐aminoethyl) pyrrolidine) were used to open the ring of the thiolactone side groups to introduce abundant functional groups (hydroxyl, carboxyl, and pyrrolidine groups) for anchoring of template proteins in the imprinting polymerization because of the excellent hydrogen bonding and hydrophobic interactions between these functional groups and proteins according to our previous study. [ 21 ] The ring‐opening process meanwhile produced sufficient thiols for grafting of functional polymers (MS@PTL‐RP). Finally, a coumarin‐terminated temperature‐responsive functional polymer PNIPAM (Coumarin‐PNIPAM‐Br) was grafted onto the surface of MS@PTL‐RP to obtain MS@PTL‐g‐PNIPAM through a thiol‐bromine click reaction.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Sequentially Controlled Recognition of Different Proteins Using Programmable Protein Imprinted Nanospheres

Wang

Zhang

et al. 2023

Small

Self Cite

View full text Add to dashboard Cite

Although protein imprinted materials with multiple templates are developed to selectively separate different proteins, it is difficult to achieve the programmed adsorption and separation of different proteins using one material, because the available protein imprinted materials are constructed through irreversible crosslinking and their structures are unprogrammable and non‐reconstructive. Herein, a novel nanosphere (MS@PTL‐g‐PNIPAM) is designed, which not only is temperature and pH responsive but also can dynamically reversibly crosslink/de‐crosslink under ultraviolet light of different wavelengths. With the help of the dynamically reversible photo‐crosslinking, the nanospheres can be repeatedly programmed into protein imprinted nanospheres toward different target proteins. Moreover, the prepared imprinted nanospheres can easily achieve the controlled rebinding and release of target proteins, benefiting from the introduced temperature‐ and pH‐responsive moieties. As a consequence, this study realizes the specific separation of different target proteins from protein mixture and the real bovine blood sequentially by programming one material. It is resource saving, time saving, recyclable, and it will provide convenience for protein imprinted materials to use in the blood purification, drug delivery, and virus detection.

show abstract

Section: Synthesis and Characterization Of Ms@ptl-g-pnipamsupporting

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Sequentially Controlled Recognition of Different Proteins Using Programmable Protein Imprinted Nanospheres

Wang

Zhang

et al. 2023

Small

Self Cite

View full text Add to dashboard Cite

show abstract

Preparation and specific adsorption of dual antibacterial BSA surface imprinted GO‐PEI/MXene wrinkled microspheres

Lyu

Yang

et al. 2023

AIChE Journal

Self Cite

View full text Add to dashboard Cite

Molecular imprinting technology has gained increasing attention and application in protein adsorption and separation. Bacterial growth on the imprinted material would reduce the adsorption selectivity and shorten the service life. To solve the above problems, carrier materials (GO‐PEI/MXene wrinkled microspheres) with dual antibacterial ability are constructed for the first time. BSA surface imprinted GO‐PEI/MXene (GO‐PEI/MXene@MIPs) are constructed in this study. The large exterior surface area increases the number of imprinting sites, thereby enhancing the adsorption capacity. The adsorption capacity of GO‐PEI/MXene@MIPs reaches 312.63 ± 22 mg/g with an imprinting factor (IF) of 3.15 within 90 min. Meanwhile, GO‐PEI/MXene@MIPs can separate BSA from mixed protein solution and fetal bovine serum (FBS) and only loses 14.1% of saturation adsorption after eight cycles. In addition, antibacterial tests show that the material has excellent broad‐spectrum antibacterial effects, which will significantly extend the service life of the material in real‐world environments.

show abstract

Light-controlled protein imprinted nanospheres with variable recognition specificity

Wang,

Fa,

et al. 2025

Chinese Chemical Letters

View full text Add to dashboard Cite

Ring -opening of polythiolactones to construct protein-imprinted nanospheres with high recognition and regulation capabilities

Abstract: Developing and preparing novel protein imprinted nanomaterials with high recognition ability remains challenging because it is difficult to controllably and orderly design functional groups and arrangement of functional groups of...

Cited by 3 publications

References 46 publications

Sequentially Controlled Recognition of Different Proteins Using Programmable Protein Imprinted Nanospheres

Sequentially Controlled Recognition of Different Proteins Using Programmable Protein Imprinted Nanospheres

Preparation and specific adsorption of dual antibacterial BSA surface imprinted GO‐PEI/MXene wrinkled microspheres

Light-controlled protein imprinted nanospheres with variable recognition specificity

Contact Info

Product

Resources

About