RIOK3 Is an Adaptor Protein Required for IRF3-Mediated Antiviral Type I Interferon Production

Feng, Jun; Jesus, Paul D. De; Su, Victoria; Han, Stephanie; Gong, Danyang; Wu, Nicholas C.; Tian, Yuan; Li, Xudong; Wu, Ting; Chanda, Sumit K.; Sun, Ren

doi:10.1128/jvi.00643-14

Cited by 46 publications

(38 citation statements)

References 51 publications

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“…In contrast, yeast cells depleted of RIOK-2 do not feature any stage specific cell cycle arrest; however, they show accelerated mitotic exit and a correlated increase in the degradation of the cell cycle regulator cyclin B1 [ 6 ]. Recently, RIOK-3 was shown to be an adapter protein required for NF-κB signaling [ 19 ] and for antiviral immune responses via the type I interferon pathway [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans

et al. 2015

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RIO protein kinases (RIOKs) are a relatively conserved family of enzymes implicated in cell cycle control and ribosomal RNA processing. Despite their functional importance, they remain a poorly understood group of kinases in multicellular organisms. Here, we show that the C. elegans genome contains one member of each of the three RIOK sub-families and that each of the genes coding for them has a unique tissue expression pattern. Our analysis showed that the gene encoding RIOK-1 (riok-1) was broadly and strongly expressed. Interestingly, the intestinal expression of riok-1 was dependent upon two putative binding sites for the oxidative and xenobiotic stress response transcription factor SKN-1. RNA interference (RNAi)-mediated knock down of riok-1 resulted in germline defects, including defects in germ line stem cell proliferation, oocyte maturation and the production of endomitotic oocytes. Taken together, our findings indicate new functions for RIOK-1 in post mitotic tissues and in reproduction.

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Section: Introductionmentioning

confidence: 99%

Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans

et al. 2015

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“…RIOK3 is a novel regulator of the antiviral type I interferon pathway and plays a crucial role in the antiviral type I interferon pathway. However, type I interferon is involved in the innate immune response which functions as the first line of defense and limits infectious pathogens directly [ 34 ], therefore we predicted that RIOK3-like is related closely to innate immunity. Mitogen-activated protein kinase kinase 6 (MP2K6), a member of the MAPK family which are signal transduction mediators that have been implicated in cell survival and death [ 35 ], is also activated during engagement of the Type I IFN receptor and plays important roles in Type I IFN signaling and generation of IFN responses [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge

Gao

Liao

Wang

et al. 2015

IJMS

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Vibrio splendidus is identified as one of the major pathogenic factors for the skin ulceration syndrome in sea cucumber (Apostichopus japonicus), which has vastly limited the development of the sea cucumber culture industry. In order to screen the immune genes involving Vibrio splendidus challenge in sea cucumber and explore the molecular mechanism of this process, the related transcriptome and gene expression profiling of resistant and susceptible biotypes of sea cucumber with Vibrio splendidus challenge were collected for analysis. A total of 319,455,942 trimmed reads were obtained, which were assembled into 186,658 contigs. After that, 89,891 representative contigs (without isoform) were clustered. The analysis of the gene expression profiling identified 358 differentially expression genes (DEGs) in the bacterial-resistant group, and 102 DEGs in the bacterial-susceptible group, compared with that in control group. According to the reported references and annotation information from BLAST, GO and KEGG, 30 putative bacterial-resistant genes and 19 putative bacterial-susceptible genes were identified from DEGs. The qRT-PCR results were consistent with the RNA-Seq results. Furthermore, many DGEs were involved in immune signaling related pathways, such as Endocytosis, Lysosome, MAPK, Chemokine and the ERBB signaling pathway.

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“…Compared with RIOK-1 and RIOK-2, much less is known about RIOK-3. The function of RIOK-3 is different from those of RIOK-1 and RIOK-2 in human cells, with RIOK-3 controlling tumor cell invasiveness, suppressing the activity of NF-κB and supporting type I-interferon production [13,14,53,54]. Recently, human RIOK-3 was discovered in ribosomal 40S subunit particles [10].…”

Section: Discussionmentioning

confidence: 99%

“…Analyses of RIOK-1 and RIOK-2 in a complex with ATP [6,55] reveal that the putative ATP binding motif, along with the beta strand-three, actually participate in binding ATP and that the Lys (K) located in the beta strand-three is required for this interaction. Site mutation changing the Lys (K) in the beta strand-three to Ala (A) in the ATP binding motifs of RIOK-2 and RIOK-3 in yeast and human cells ablate the ATP binding activity and affect the kinase functions of these enzymes [54,56]. Although the Lys (K) from the ATP binding loop does not directly interact with ATP, it is essential for RIOK-2 to bind with pre-40S subunit to participate in ribosomal biogenesis [56].…”

Section: Discussionmentioning

confidence: 99%

Exploring features and function of Ss-riok-3 , an enigmatic kinase gene from Strongyloides stercoralis

Wang

Liu

Lok

et al. 2014

Parasites & Vectors

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Background: Right open reading frame protein kinase 3 (RIOK-3) belongs to the atypical kinase family. Unlike the other two members, RIOK-1 and RIOK-2, which are conserved from Archaea to humans, RIOK-3 occurs only in multicellular organisms. Studies on HeLa cells indicate that human RIOK-3 is a component of the 40S small ribosome subunit and supports cancer cell growth and survival. However, almost nothing is known about the function of RIOK-3. We explored the functional role of RIOK-3 encoding gene from Strongyloides stercoralis, a parasitic nematode of humans and dogs. Methods: To analyze the gene and promoter structure of Ss-riok-3, RACE-PCR and Genome-walker PCR were performed to isolate the full length cDNA, gDNA and promoter region of Ss-riok-3. RNA-seq was conducted to assess the transcript abundance of Ss-riok-3 in different stages of S. stercoralis. Transgenesis was employed to determine the anatomic expression patterns of Ss-riok-3.

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RIOK3 Is an Adaptor Protein Required for IRF3-Mediated Antiviral Type I Interferon Production

Cited by 46 publications

References 51 publications

Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans

Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans

Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge

Exploring features and function of Ss-riok-3 , an enigmatic kinase gene from Strongyloides stercoralis

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