Rise of the Animal Kingdom and Epigenetic Mechanisms of Evolution

Cabej, Nelson R.

doi:10.1016/b978-0-12-401667-5.00005-5

Cited by 3 publications

(1 citation statement)

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“…Many genes whose expression peaks singly at ZT14 have functions related to actin cytoskeleton reorganisation and/or to small GTPase signalling ( Figure 2—figure supplement 1F–G ). This supports the hypothesis that actin depolymerisation is important for phase delays but not phase advances ( Gerber et al, 2013 ; Cabej, 2013 ). Taken together, our data indicate that oscillations in the SCN transcriptome play an important role in gating the differential responses to light, a fundamental circadian process.…”

Section: Resultssupporting

confidence: 86%

Temporal transcriptomics suggest that twin-peaking genes reset the clock

Pembroke

Babbs

Davies

et al. 2015

eLife

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The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light / dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN. Another group of 766 genes unexpectedly peaked twice, near both the start and end of the dark phase; this twin-peaking group is significantly enriched for synaptic transmission genes that are crucial for light-induced phase shifting of the circadian clock. 341 intergenic non-coding RNAs, together with novel exons of annotated protein-coding genes, including Cry1, also show specific circadian expression variation. Overall, our data provide an important chronobiological resource (www.wgpembroke.com/shiny/SCNseq/) and allow us to propose that transcriptional timing in the SCN is gating clock resetting mechanisms.DOI: http://dx.doi.org/10.7554/eLife.10518.001

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Section: Resultssupporting

confidence: 86%