Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques

Mazari, Wissame; Boucherit-Otmani, Z.; Haci, Imad Abdelhamid El; Ilahi, Amine; Boucherit, K.

doi:10.1111/idj.12401

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…Fungi have also been found in water from dental units. In a study conducted by Mazari et al [24], 18 dental waterlines were analyzed for the presence of yeasts on their internal surfaces. Of the 18 DUWLs studied, 10 were contaminated (55.56%).…”

Section: Microbial Contamination Of Duwls and Biofilmsmentioning

confidence: 99%

Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review

2020

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Several studies have revealed that dental unit waterlines (DUWLs) are often contaminated by large numbers of various micro-organisms (bacteria, fungi, protozoa, viruses). Microbial contamination in DUWLs may originate from the mains water piped into the dental unit, the suck-back of patients’ saliva into the line due to the lack of adequate valves, and contamination from bottled water systems. Some of the main determinants of microbial contamination in DUWLs are: a very small lumen size (0.5–2 mm) of the tubing used, high surface-to-volume ratio (6:1), low throughput and the materials of which the tubing is made, water stagnation outside of working hours. The environmental conditions present inside the conduits of the dental unit may facilitate the proliferation of micro-organisms and the consequent formation of biofilm on the interior surface of the pipes of DUWLs. During the use of handpieces, particularly high-speed rotating instruments, a spray is thrown up in the form of aerosols or spatters containing biological material (saliva, blood and dental plaque) and micro-organisms. This means that the health of both dental staff and patients could be at risk of infection. The risk of cross-infections in dental settings can be tackled by implementing combined interventions to prevent the contamination of DUWLs.

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Section: Microbial Contamination Of Duwls and Biofilmsmentioning

confidence: 99%

Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review

2020

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“…Biofilms up to 50-mm thick have been found in functioning dental units, composed of complex microbial communities (Porteous et al, 2011). Yeasts once identified in DUWL especially Candida can form biofilms and resist antifungal agents (Mazari et al, 2018). As a result, no matter how various procedures were applied, none of these can stop biofilm from accumulating and detaching.…”

Section: Introductionmentioning

confidence: 99%

Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties

Fan

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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The contamination of dental unit waterlines (DUWLs) is a serious problem and directly affects the dental care. This study aims to explore the microbial community of biofilm in DUWL from different specialties and investigate the associated factors. A total of 36 biofilm samples from 18 DUWL of six specialties (i.e., prosthodontics, orthodontics, pediatrics, endodontics, oral surgery, and periodontics) at two time points (i.e., before and after daily dental practice) were collected with a novel method. Genomic DNA of samples was extracted, and then 16S ribosomal DNA (rDNA) (V3–V4 regions) and ITS2 gene were amplified and sequenced. Kruskal–Wallis and Wilcoxon rank test were adopted for statistical analysis. Microbial community with high diversity of bacteria (631 genera), fungi (193 genera), and viridiplantae was detected in the biofilm samples. Proteobacteria was the dominant bacteria (representing over 65.74–95.98% of the total sequences), and the dominant fungi was Ascomycota (93.9–99.3%). Microorganisms belonging to multiple genera involved in human diseases were detected including 25 genera of bacteria and eight genera of fungi, with relative abundance of six genera over 1% (i.e., Acinetobacter, Pseudomonas, Enterobacter, Aspergillus, Candida, and Penicillium). The biofilm microbiome may be influenced by the characteristics of dental specialty and routine work to some extent. The age of dental chair unit and overall number of patients had the strongest impact on the overall bacteria composition, and the effect of daily dental practices (associated with number of patients and dental specialty) on the fungi composition was the greatest. For the first time, biofilm in DUWL related to dental specialty was comprehensively evaluated, with more abundance of bacterial and fungal communities than in water samples. Biofilm accumulation with daily work and multiple kinds of opportunistic pathogen emphasized the infectious risk with dental care and the importance of biofilm control.

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“…Water from DU has also been discovered to contain fungi. In a study directed by (Mazari et al, 2018), 18 dental waterlines were assayed for the existence of yeasts in their internal areas. Of the 18 DUWLs studied, 10 were polluted (55.56%).…”

Section: Microbial Contamination Of Duwls and Biofilmsmentioning

confidence: 99%

Dental unit waterlines and health risks of pathogenic microbial contamination: An update review

Hikal,

Kačániova,

Hussein

et al. 2023

JBS

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Recently, there has been great concern about the levels of oral diseases associated with inappropriate and unsanitary oral care methods. In this line, the most serious is the water contamination with pathogenic microbes. Owing to the water microbial contamination of dental units, patients' oral health is threatened as a result of systemic disorders. Patients receiving treatment in dental unit waterlines were found to be affected by dangerous bacteria. Since both clients and dental staff are exposed to aerosols and water from dental equipment, the water should be of great quality. Due to the fact that dental unit waterlines (DUWLs) create a suitable environment for microbial development and the formation of biofilms, the water is often contaminated with a high density of several microorganisms (e.g., bacteria, fungi, protozoa, viruses), causing a health problem for dentists, patients and elderly patients, especially those with immunocompromised. This has fueled interest in presenting the current situation to shed light on (i) the microbial contamination of DUWLs and (ii) the connected infectious risks. It is essential that an elevated degree of mouth care be combined with adequate water quality and preventive dental units to maintain oral comfort, prevent deterioration, and promote public health. The growing population of individuals with compromised immune systems and the latest technological advancements concerning biofilms, water quality, etc. necessitate the implementation of improved management practices and the application of decontamination protocols for waterlines.

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Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques

Abstract: Several types of yeasts were identified; the most frequently isolated genus was Candida. The majority of these yeasts had the ability to form biofilms and resisted antifungal agents used in this study.

Cited by 8 publications

References 40 publications

Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review

Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review

Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties

Dental unit waterlines and health risks of pathogenic microbial contamination: An update review

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