Heavy metals as environmental pollutants have been recognized to have a role in induction of malignant human growths. Recently, certain heavy metals showed a close association to breast cancer. This research was conducted to find out the role of some toxic heavy metals (cadmium, iron, copper, lead and zinc) in induction of breast cancer in-vivo. The study was carried out on 100 female patients: 75 with breast cancer (cancerous group) and 25 with benign breast diseases (non-cancerous group). Patients were chosen from those attending to the Oncology Center, Mansoura University. Heavy metals concentrations were measured in the urine and breast tissue samples using inductive coupled plasma (ICP)-spectrometer. The present results showed a significant increase in urine and tissue cadmium concentrations and urine copper concentration in cancerous patients compared to their corresponding non-cancerous ones (p < 0.05). Also, there was a significant reduction in iron concentration in urine samples of cancerous group compared to their corresponding non-cancerous one (p < 0.05). On the other hand, lead had no significant difference between cancerous and non cancerous groups but it was generally high in the tissue samples while zinc had no significant difference between studied groups. It could be concluded that the present study posits a causal association between cadmium and copper increase with reduction of iron and breast cancer. were more prevalent in women. The present study is designed to find out the role of some toxic heavy metals (cadmium, iron, copper, lead and zinc) in induction of breast cancer in-vivo. Materials and Methods This study was carried out on 100 female patients attending to the Oncology Center, Mansoura University, Dakahlia Governorate, Egypt during the period from December 2008 till December 2009. Their ages ranged from 30-70 years. Relevant information was obtained from each patient before surgery regarding residence, occupational history, smoking habits and reproductive history. Patients with positive family history of breast cancer were excluded from the study. Informed consent was obtained from each patient. Study groups Patients were divided into two groups. A cancerous group (75 female patients) with histologically confirmed breast cancer lesions. A non-cancerous group (25 female patients) with non risky non proliferative benign breast diseases that served as a control group. Study design According to Ionescu [11], 10 ml urine sample and 10 gm of breast adipose tissue were obtained from each patient. Samples were Jour n a l o f C linical T o x ic o log y