Risk Assessment of Gastric Cancer Caused by Helicobacter pylori Using CagA Sequence Markers

Zhang, Chao; Xu, Shaofa; Xu, Dong

doi:10.1371/journal.pone.0036844

Cited by 17 publications

(12 citation statements)

References 37 publications

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“…In order to quantify the degree of epiallelic shift, we applied combinatorial entropy [15,16,21], a measure to compare the difference distributions of the epialleles between different stages. The estimated change between stage k = 1 and stage k = 2 at locus j were defined by S j , which is the sum of the nature logarithm transformation of the total number of permutations Z k,j :…”

Section: Foreground Combinatorial Entropymentioning

confidence: 99%

Dynamic evolution of clonal epialleles revealed by methclone

Li¹,

Garrett-Bakelman²,

Perl³

et al. 2014

Genome Biol

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We describe methclone, a novel method to identify epigenetic loci that harbor large changes in the clonality of their epialleles (epigenetic alleles). Methclone efficiently analyzes genome-wide DNA methylation sequencing data. We quantify the changes using a composition entropy difference calculation and also introduce a new measure of global clonality shift, loci with epiallele shift per million loci covered, which enables comparisons between different samples to gauge overall epiallelic dynamics. Finally, we demonstrate the utility of methclone in capturing functional epiallele shifts in leukemia patients from diagnosis to relapse. Methclone is open-source and freely available at

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Section: Foreground Combinatorial Entropymentioning

confidence: 99%

Dynamic evolution of clonal epialleles revealed by methclone

Li¹,

Garrett-Bakelman²,

Perl³

et al. 2014

Genome Biol

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“…Thus, these antigens are regarded as prospective candidates that may serve as infection markers (Robinson et al, 2007). Various H. pylori antigens, such as CagA, VacA, HspB, FlaA, FlaB and urease subunits (UreA, UreB), have been employed as diagnostic markers of infection (Widmer et al, 1999;Cremonini et al, 2004;Schumann et al, 2006;Zhang et al, 2012;Flores-Luna et al, 2013;Noto et al, 2013). Although several studies tried to establish an association between well-known virulence markers and clinical outcomes, the results were not conclusive.…”

Section: Introductionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (≥ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culturepositive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/ TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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“…11,12 . It cause dyspepsia, acute gastritis, chronic gastritis, peptic ulcer, MALT-lymphoma and gastric adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Helicobacter Pylori is associated with decrease serum level of the thyroid hormonal in healthy elderly population

Suwarni¹,

Cilmiaty²,

Indarto³

et al. 2017

Bangladesh J Med Sci

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Background:Helicobacter pylori infection is the most prevalence infectious disease as it affects more than half of the world population and causes chronic cellular inflammatory response in the gastric mucosa. Helicobacter pylori infection has been epidemiologically proven to be linked to extra-digestive conditions and disease. It has been speculated that H.pylori infection may be responsible for various endocrine disorders. The thyroid may be one of the targets of Helicobacter pylori chronic inflammation. Here we sought too investigate whether H.pylori infections were associated with decrease level of the thyroid hormonal. Methods: This study involved elderly aged 50-90 years who had visited a health promotion center for elderly. A total 101 euthyroid subjects were been enrolled in this cross-sectional study. Diagnosed of Helicobacter.pylori infections by ELISA of Ig G antibodies of Helicobacter pylori. We examine serum T3 level and serum TSH level by ELEXIS. For statistical method we use Pearson bivariat analysis to determine the association of two variable,and linier regression to determine which variable is more influented by Helicobacter pylori. Results: Fourty-two (41,6 %) subjects had been diagnosed with H.pylori infections. Pearson bivariat analysis showed that Helicobacter pylori infection was significantly associated with decreased serum T3 level ( correlations coefficient r = -0,66 ,p< 0,001 ). The prevalence of Helicobacter pylori infection showed a increasing trend as serum TSH level decreased (correlations coefficient r = -0,53, p < 0,001). Linier regression analysis showed thatHelicobacter pylori infection was significantly associated with the risk of decreased thyroid hormonal fuction ( B = -0,272. R 2 = 0,676. P < 0.001 ). Conclusion: Our results suggested that H.pylori infections were significantly associated with the decreased serum level of T3 and TSH serum level in the healthy elderly population, whose thyroid functions were in the reference range.

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Risk Assessment of Gastric Cancer Caused by Helicobacter pylori Using CagA Sequence Markers

Cited by 17 publications

References 37 publications

Dynamic evolution of clonal epialleles revealed by methclone

Dynamic evolution of clonal epialleles revealed by methclone

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Helicobacter Pylori is associated with decrease serum level of the thyroid hormonal in healthy elderly population

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