Risk factors and antimicrobial resistance profiles of Pseudomonas putida infection in Central China, 2010–2017

Abstract

The aim of this study was to analyze the risk factors, clinical features, and antimicrobial resistance of Pseudomonas putida (P putida) isolated from Tongji Hospital in Wuhan, China. The data of 44 patients with P putida infections were retrospectively reviewed in this study. All cases of P putida strains were detected by the clinical laboratory of Tongji Hospital in the period of January 2010 to December 2017. Antimicrobial susceptibility testing was conducted using Kirby-Bauer method. Forty-four effective strains of P putida were isolated, including 32 inpatients and 12 outpatients. The 32 inpatients cases were obtained from various departments, which were urosurgery wards (n = 5, 15.6%), pediatrics wards (n = 4, 12.5%), hepatic surgery wards (n = 4, 12.5%), among others. The isolates had been discovered from urine specimens (28.2%), blood specimens (21.9%), sputum specimens (12.5%), and so on. Twenty-five patients had histories of catheterization before the isolation of P putida. Twenty-four patients were in immunocompromised states, 5 patients had undergone surgery, catheterization and were taking immunosuppressive therapy simultaneously. Polymicrobial infections were found in some P putida cases, especially Stenotrophomonas maltophilia, Pseudomonas aeruginosa, and Escherichia coli. All the patients had treated by antimicrobial before culture. Multi-drug-resistant strains were detected in 75% of P putida isolates. The P putida strains were resistant to trimethoprim/sulfamethoxazole (97.7%), aztreonam (88.6%), minocyline (74.3%), ticarcillin/clavulanic acid (72.7%), and sensitive to amikacin (86.4%), imipenem (62.8%), gentamicin (56.8%). Catheterization or other invasive procedures, immunocompromised states, and underlying diseases increased the risks of P putida infections. Moreover, the P putida strains were highly resistant to trimethoprim/sulfamethoxazole, aztreonam, minocyline, ticarcillin/clavulanic acid.

Bactericidal Activity of Non-Cytotoxic Cationic Nanoparticles against Clinically and Environmentally Relevant Pseudomonas spp. Isolates

“…Despite this, many studies have reported that P. putida is the cause of severe illness in people with compromised immune systems, including a lethal case of bacteremia [ 48 ]. A recent study developed to analyze the risk factors, clinical characteristics, and antimicrobial resistance of P. putida isolated from Tongji Hospital in Wuhan (China) found high rates (75%) of drug resistance in P. putida isolates, particularly to trimethoprim/sulfamethoxazole (97.7%), aztreonam (88.6%), minocilin (74.3%), and ticarcillin/clavulanic acid (72.7%) [ 49 ].…”

RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum

“…Since P. putida is typically soil and/or aquatic bacterium, the focus of the research was on TCSs enabling tolerance to pollutants and plant colonization [ 11 , 12 , 14 , 15 , 19 ]. Albeit rarely, P. putida could also cause different types of infections in human beings [ 20 , 21 , 22 ]. Of special importance was often described multidrug-resistant phenotype of these isolates [ 20 , 21 , 22 ], while the pathogenic potential was isolate-specific [ 23 ].…”

Molecular diagnostics and next-generation sequencing reveal real etiological characteristics of invasive Salmonella infection in febrile illness in Freetown, Sierra Leone

“…This illustrates the demand for clear etiological discrimination in patients with fever. P. putida is widely found on hospital surfaces and moist environments and, as an opportunistic pathogen, can cause bloodstream infections [ 26 , 27 ]. Therefore, bloodstream infection surveillance is essential to characterize the public health threat [ 14 ].…”