Risk factors associated with Salmonella prevalence, its antibiotic resistance, and egg antibiotic residues in the layer farming environment

Abstract

Background and Aim: Human salmonellosis with non-typhoidal Salmonella remains a global public health concern related to the consumption of contaminated eggs and egg-based products. This study aimed to examine the prevalence of Salmonella, antimicrobial-resistant Salmonella, and egg antibiotic residues concerning risk factors associated with Salmonella contamination in eggs, the layer farming environment, and laying hens kept in battery-cage closed-housing systems. Materials and Methods: This study used a repeated cross-sectional design to collect 488 samples from eggs, laying hens, and the farm environment on one laying farm for Salmonella detection according to ISO 6579:2002/AMD 1:2007. Salmonella-positive samples were further tested for serotype and antimicrobial susceptibility using the disk diffusion test. The layer farm contact person was interviewed at the sampling time to evaluate the risk factors associated with Salmonella contamination using logistic regression analysis. For each month, 24 eggs (144 eggs in total) were also randomly sampled from the collection egg area at the farm for antibiotic residue detection using the European Four Plate Test. Results: The highest Salmonella prevalence rates were in the samples from the layer pen floors, followed by the egg sizing machine (ESM) and eggshells at 65.5%, 52.5%, and 15%, respectively. Salmonella enterica serovar Corvallis was the dominant serovar (48.38%), followed by Mbandaka (37.76%), Braenderup (14.29%), and Typhimurium (4.08%). Rodent presence at the farm and the frequency of changing the disinfectant foot dip were significant factors related to Salmonella contamination on the pen floors (odds ratio [OR]=22.5, 95% confidence interval [CI]=2.11-240.48, p=0.01; OR=24, 95% CI=2.78-206.96, p=0.004, respectively). Hand-washing before sorting and cleaning the ESM were the significant factors (OR=13, 95% CI=1.2-140.73, p=0.04). The most resistant Salmonella isolates were resistant to oxytetracycline. One isolate of S. enterica Typhimurium was resistant to cefotaxime, enrofloxacin, and oxytetracycline. The antibiotic residues in the egg yolks were streptomycin, enrofloxacin, and tetracycline at prevalence rates of 36.11%, 11.81%, and 7.64%, respectively. Streptomycin was the most abundant residue in the albumen and yolk, followed by tetracycline. Conclusion: Salmonella prevalence in layer farming with a closed-housing system is related to effective biosecurity and hygiene issues, such as rodent control, clean farm equipment, and good worker hygiene. In addition, eggs' antibiotic residues may be related to treating antimicrobial-resistant Salmonella isolates and medicated feed with inappropriate antibiotic withdrawal time.

The phenotypic and genotypic antimicrobial resistance patterns of Salmonella isolated from chickens and meat at poultry slaughterhouses in Japan and Thailand

“…These results demonstrated that tetracycline derivatives, such as DO or oxytetracycline, are being used and may be misused or overused for treating poultry diseases in farms in both countries. This finding is similar to those of previous studies by Duc et al [6], Shimojima et al [10] and Sornplang et al [19]. In this study, the Salmonella strains from Thailand also had high phenotypic resistance to CT, which was the second-most common form of resistance.…”

“…This indicates that Salmonella contamination likely occurred during the slaughtering processes and may have come from the slaughterhouse environment and evisceration areas as indicated by the three most commonly identified serovars in this study of Corvallis, Enteritidis, and Typhimurium. These Salmonella serovars have been detected primarily in poultry farm environments and poultry meat [ 19 , 20 ]. In addition, an effective biosecurity program used by broiler farms can decrease Salmonella cross-contamination among broilers, humans, the environment, and broiler meat during the slaughtering process [ 21 ].…”

“…[ 10 ] and Sornplang et al . [ 19 ]. In this study, the Salmonella strains from Thailand also had high phenotypic resistance to CT, which was the second-most common form of resistance.…”

Integration of water, sanitation, and hygiene program with biosecurity: A One Health approach to reduce the prevalence and exposure of antibiotic-resistant bacteria in the livestock community

“…(3) using farm clothing and personal protection equipment (PPE) to practice personal hygiene, along with cleaning body parts in contact before and after work [75,76]; (4) periodically inspecting equipment and PPE [77]; and (5) scheduling the washing of large livestock [78]. Depending on the standard procedures Available at www.onehealthjournal.org/Vol.9/No.2/16.pdf used, various cleaning techniques were applied.…”