ABSTRAKPeriferal intravena kateter (PIK) & Health 2016;11(2): 181-188 Ho S.E. et al. highlighted that nurses' awareness of infusion-related complications can significantly affect patients' outcomes and health care costs. The study also emphasized that complications of intravenous therapy are costly in terms of patient quality of life, morbidity, mortality, treatment expense, and extended length of hospital stay. According to the World Health Organization (WHO 2009), routine hand washing in patient care is to remove dirt and organic material as well as micro-bacterial contamination acquired through contact with patients or the environment. A study by Hadaway (2012a) claimed that nurses' lack of standardization in practice and knowledge of PIC care directly affects patients' safety risks and outcomes. There were instances where patients nurses' knowledge and practice towards care of PIC. A cross-sectional descriptive study was conducted and 84 respondents participated in the study. A 26-item questionnaire comprising 11 items on knowledge and 15 items related to nursing practice was adapted and modified. The findings reported higher mean score from specialty unit respondents, with knowledge (M = 49.19, SD = 3.44) and practice (M = 66.38, SD = 5.15), compared to medical surgical wards with knowledge (M = 46.25, SD = 4.68) and practice (M = 63.17, SD = 4.63) towards the care of PIC, which was significant (p value = 0.010 and p value = 0.009, respectively). The study indicated a higher mean score of charge nurse respondents as compared with registered nurse with knowledge (M = 49.68, SD = 3.23); (M = 46.20, SD = 4.62) and practice (M = 67.11, SD = 4.84); (M = 63.06, SD = 4.61), which was significant (p value = 0.003 and p value = 0.001, respectively). There were no significant differences between respondents' education qualification towards care of PIC with p > 0.05. However, the findings reported that respondents who possessed Bachelor of Nursing were deemed to score slightly higher in their knowledge and practice towards the care of PIC. In conclusion, the specialty unit and charge nurse respondents were deemed to possess better knowledge and practice towards the care of PIC in the hospital.