Risk of ovarian failure and fertility preserving methods in girls and adolescents with a malignant disease

Schmidt, Kirsten Tryde; Larsen, EC; Andersen, C. Yding; Andersen, A.Nyboe

doi:10.1111/j.1471-0528.2009.02408.x

Cited by 104 publications

(49 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Constant progress in the diagnosis and treatments has greatly improved the life expectancy of adolescents and young women at risk of premature ovarian failure (POF) due to oncological and non-oncological disorders [13,37]. Ovarian tissue cryopreservation is the only way to preserve fertility of patients who cannot delay their treatment or who are not Capsule An original slow freezing protocol able to protect both quality and functionality of human ovarian tissue, and easily applicable in a clinical setting.…”

Section: Introductionmentioning

confidence: 99%

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Sanfilippo

Canis

Romero

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To evaluate the efficiency of an original slow freezing protocol on the quality and function of human ovarian cortex. Methods Human ovarian tissues were cryopreserved using a freezing medium supplemented with propanediol and raffinose as cryoprotectants and antioxidants (L-glutamine, taurine). Samples were then frozen using a faster cooling rate than the usual one. Viability and morphology of follicles, DNA fragmentation in follicles and stroma as well as histology of the vascular endothelium were analyzed before and after freezing/thawing. Moreover, a functional analysis was performed based on the evaluation of follicular growth and development in thawed ovarian tissues that were cultured in vitro. Results Our freezing/thawing protocol allows preservation of a high proportion of viable follicles and the preservation of the different follicle developmental stages (p>0.05 versus fresh control). 70.5±5.2 % of follicles retained an intact morphology after cryopreservation (p=0.04). Stroma cells but not follicles exhibited a slight increase of DNA fragmentation after thawing (p<0.05). Microvessel endothelium within thawed tissues appeared to be preserved. Granulosa cells showed signs of proliferation in follicles cultured for 12 days. Secretion of 17β-oestradiol significantly increased during in vitro culture. Conclusions This protocol leads to good preservation of ovarian integrity and functionality post-thawing and thus appears as a suitable technique of ovarian tissue cryopreservation in clinical settings. Further research could be extended to optimize conditions of in vitro culture.

show abstract

Section: Introductionmentioning

confidence: 99%

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Sanfilippo

Canis

Romero

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…However, these ART procedures require hormonal stimulation, which can both delay treatment of the disease and create a risk for some patients with hormone-responsive diseases [12]. Furthermore, these methods are generally not applicable to pre-pubertal girls [12,13]. For these populations, ovarian tissue transplantation is a promising investigational technique.…”

Section: Introductionmentioning

confidence: 99%

Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Laronda

Duncan

Hornick

et al. 2014

J Assist Reprod Genet

114

View full text Add to dashboard Cite

“…The pool of ovarian follicles that constitute the reproductive potential of a girl may be severely reduced or disappear as a consequence of the treatment required (4). In prepubertal girls the only available option to preserve fertility is to cryopreserve ovarian tissue (5,6). If ovarian activity is destroyed and premature ovarian insufficiency (POI) occurs in young female survivors, cortical ovarian tissue might be transplanted to restore ovarian function for fertility purposes (7,8).…”

mentioning

confidence: 99%

Effect of First Line Cancer Treatment on the Ovarian Reserve and Follicular Density in Girls Under the Age of 18 Years

Issaoui

Giorgione

Mamsen

et al. 2017

Obstetrical &Amp; Gynecological Survey

View full text Add to dashboard Cite

Objective: To study the impact of first-line antineoplastic treatment on ovarian reserve in young girls returning for ovarian tissue cryopreservation (OTC) in connection with a relapse. Design: Retrospective case-control study. Setting: University hospitals. Patient(s): Sixty-three girls under the age of 18 years who underwent OTC before (group 1: 31 patients) and after (group 2: 32 patients) their initial cancer treatment. Intervention(s): None. Main Outcome Measure(s): Follicular densities (follicles/mm 3 ) measured from an ovarian cortical biopsy before OTC. The ovarian volume (mL) of entire ovaries excised for OTC was also monitored. Result(s): There was no statistically significant difference in the mean age or follicular density between groups 1 and 2 (334 AE 476/ mm 3 vs. 327 AE 756/mm 3 ). In contrast, the ovarian volume and total number of ovarian cortex chips cryopreserved were statistically significantly lower in patients who received gonadotoxic treatment before OTC (mean AE standard deviation [SD]: ovarian volume, 5.3 AE 3.1 mL vs. 2.9 AE 2.1 mL, respectively; number of cortex chips: 21.3 AE 8.1 vs. 15.2 AE 7.1, respectively). The reduction in the estimated ovarian reserve ranged from 10% to 20% in children to around 30% in adolescent girls (>10 years). Conclusion(s): Girls under the age of 10 tolerate a gonadotoxic insult better than adolescents, who may experience up to a 30% reduction in the ovarian reserve via first-line gonadotoxic treatment, which at present is considered to have little effect on the follicle pool. This information will improve counseling of young female cancer patients in deciding whether to undergo fertility preservation treatment. (Fertil Steril Ò 2016;106:1757-62.

show abstract

Risk of ovarian failure and fertility preserving methods in girls and adolescents with a malignant disease

Cited by 104 publications

References 92 publications

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Effect of First Line Cancer Treatment on the Ovarian Reserve and Follicular Density in Girls Under the Age of 18 Years

Contact Info

Product

Resources

About