2010
DOI: 10.1002/jbio.201000036
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RMieS‐EMSC correction for infrared spectra of biological cells: Extension using full Mie theory and GPU computing

Abstract: In the field of biomedical infrared spectroscopy it is often desirable to obtain spectra at the cellular level. Samples consisting of isolated single biological cells are particularly unsuited to such analysis since cells are strong scatterers of infrared radiation. Thus measured spectra consist of an absorption component often highly distorted by scattering effects. It is now known that the predominant contribution to the scattering is Resonant Mie Scattering (RMieS) and recently we have shown that this can b… Show more

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Cited by 124 publications
(147 citation statements)
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“…A variety of different terms such as "dispersion artefact", "anomalous dispersion", or "(Resonant) Mie scattering" can be found in the literature to describe the wealth of scattering-induced spectral alterations. We and others 22 believe that "Resonant Mie scattering" should preferably be used to describe such effects because this term includes both, the presence of Mie scattering and the fact that the real and the imaginary part vary together in "resonance" in the region of an absorption band. Other terms ("dispersion artefact", "anomalous dispersion" 7,17 ) should be avoided in the future because they more likely confuse than clarify the complex subject.…”
Section: Discussionmentioning
confidence: 99%
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“…A variety of different terms such as "dispersion artefact", "anomalous dispersion", or "(Resonant) Mie scattering" can be found in the literature to describe the wealth of scattering-induced spectral alterations. We and others 22 believe that "Resonant Mie scattering" should preferably be used to describe such effects because this term includes both, the presence of Mie scattering and the fact that the real and the imaginary part vary together in "resonance" in the region of an absorption band. Other terms ("dispersion artefact", "anomalous dispersion" 7,17 ) should be avoided in the future because they more likely confuse than clarify the complex subject.…”
Section: Discussionmentioning
confidence: 99%
“…[6][7][8]15,19 The models developed in these studies have been incorporated into computer algorithms to minimise scattering effects in contaminated IR absorption spectra. For example, Bassan et al applied the van de Hulst approximation 8 and in a subsequent study an improved model with the full Mie scattering formalism for separating scattering and pure absorption contributions from experimental IR spectra 22 . Although these efforts have proved to be exceptionally useful for many practical applications, it cannot be overlooked that the scattering correction routines are rather complex and often not accessible to the practitioners.…”
Section: Discussionmentioning
confidence: 99%
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“…M ore recently, we developed a correction algorithm to tackle this problem that enables the scattering component of the spectrum to 5 be calculated and removed from the measured spectrum, leaving the pure absorption spectrum of the intact cell. [27][28] This correction algorithm has been used successfully on single cell spectra enabling different cell types, including stem-like cells, to be separated based on their infrared spectral features. 29 therefore, that spectral separation between cell phenotypes may be based in-part on changing cell morphology rather than exclusive changes in cell chemistry.…”
Section: Introductionmentioning
confidence: 99%