RmtC and RmtF 16S rRNA Methyltransferase in NDM-1–Producing<i>Pseudomonas aeruginosa</i>

Rahman, Mohibur; Prasad, Kashi Nath; Pathak, Ashutosh; Pati, Binod Kumar; Singh, Avinash; Ovejero, Cristina M.; Ahmad, Saheem; González‐Zorn, Bruno

doi:10.3201/eid2111.150271

Cited by 38 publications

(23 citation statements)

References 12 publications

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“…In our study, we characterized five different types of 16S rRNA methyltransferase genes responsible for aminoglycoside nonsusceptibility. Among these, rmtC was found to be the most predominant type in the northeastern part of India, which is unusual compared to the findings of other studies (2). These aminoglycoside resistance determinants were found to be associated with broad-host-range plasmids of diverse incompatibility groups, reflecting multiple sources of acquisition through lateral gene transfer.…”

Section: Discussioncontrasting

confidence: 43%

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Occurrence of Acquired 16S rRNA Methyltransferase-Mediated Aminoglycoside Resistance in Clinical Isolates of Enterobacteriaceae within a Tertiary Referral Hospital of Northeast India

Wangkheimayum

Paul

Dhar

et al. 2017

Antimicrob Agents Chemother

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The methylation of a ribosomal target leads to a high level of resistance to all clinically relevant aminoglycoside antibiotics, so early detection of these resistance determinants will help to reduce the incidence of treatment failures as well as lessen the dissemination rate. Here, we characterized different 16S rRNA methyltransferases responsible for aminoglycoside resistance and their epidemiological background in clinical isolates of Enterobacteriaceae in a tertiary referral hospital in India. All aminoglycoside-resistant isolates were screened for different 16S rRNA methyltransferases by PCR assay, and incompatibility typing of the conjugable plasmid harboring resistance genes was performed by PCR-based replicon typing. An assay for the stability and elimination of these resistance plasmids was performed. The coexistence of extended-spectrum ␤-lactamases and metallo-␤-lactamases was also detected, and the heterogeneity of these isolates was determined by enterobacterial repetitive intergenic consensus PCR. The PCR assay revealed the presence of armA, rmtA, rmtB, rmtC, and rmtD in single and multiple combinations, and these were carried by a diverse group of Inc plasmids. Plasmids harboring these resistance determinants were highly stable and maintained until the 55th serial passage, but SDS treatment could easily eliminate the plasmids harboring the resistance determinants. The coexistence of bla TEM , bla PER , bla GES , and bla SHV , as well as bla VIM and bla NDM , within these isolates was also detected. Strains with different clonal patterns of aminoglycoside resistance were found to spread in this hospital setting. We observed that the 16S rRNA methyltransferase genes were encoded within different Inc plasmid types, suggesting diverse origins and sources of acquisition. Therefore, the present study is of epidemiological importance and can have a role in infection control policy in hospital settings.

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Section: Discussioncontrasting

confidence: 43%

“…rmtH, and npmA) have been detected in various nosocomial pathogens (2). Organisms harboring 16S rRNA methyltransferases along with extended-spectrum ␤-lactamases (ESBLs) and carbapenem resistance determinants have mostly been reported (3,4).…”

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confidence: 99%

Occurrence of Acquired 16S rRNA Methyltransferase-Mediated Aminoglycoside Resistance in Clinical Isolates of Enterobacteriaceae within a Tertiary Referral Hospital of Northeast India

Wangkheimayum

Paul

Dhar

et al. 2017

Antimicrob Agents Chemother

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“…Among these, rmtB (16.39%) was found to be the most predominant type in this part of India, on the contrary, Wangkheimayum J et al, (2017) found Rmt C being predominant in the Eastern part of the India (8). It is reported that 16S rRNA methyltransferases often coexist with bla NDM and bla CTX-M genes (20) (8) and our study was not an exception. The findings that ESBL producing uropathogens co-harbored carbapenem, aminoglycoside and AmpC resistant genes underscore that India as an epicenter of horizontal transfers of high-level resistance alleles between Gram-negative bacteria irrespective of community or nosocomial settings [17] [18].…”

Section: Resultssupporting

confidence: 47%

Emergence of Multidrug-Resistant Uropathogens harboring ESBL, Carbapenem, Aminoglycosides and AmpC resistant genes from Northern India

Bhattacharjee

Paul

et al. 2018

Preprint

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25Extended-spectrum β-lactamase (ESBL) producing bacteria acts as a serious threat, and its co-existence which included bla NDM-5 (n=48), bla NDM-5 + bla OXA-48 (n=5), bla NDM-5 + bla IMP (n=5) and bla NDM-5 + 37 bla IMP + bla VIM (n=1). The ESBL producing uropathogens also harbored 16S rRNA methylase genes 38 which included rmtB (n=9), rmtA (n=4), rmtC (n=1) and ArmA (n=1) followed by AmpC genes which 39 includes CIT (n=8) and DHA-1 (n=1) genes. Imipenem and gentamicin were found to be more effective. 40We speculating, this is the first report showing the prevalence of multidrug-resistant uropathogens in 41 this area demanding regular surveillance for such resistance mechanisms which will be useful for health 42 personnel to treat ESBL infection and its co-existence with another antibiotic resistance gene.

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“…The rmtA gene in P. aeruginosa carries a mobile element Tn5041 [58,59] identified previously in Enterobacteriaceae [56], while the rmtB gene identified in Serratia marcescens is located in the flanking of Tn3-like region responsible for multiple antimicrobial resistance [59,60], and both methyltransferases and mobile elements are present in P. aeruginosa ( Table 2). The rmtC and rmtF genes ( Table 2) might have been acquired from plasmids as part of mobile genetic elements and finally integrated and stabilized on the chromosome [61]. The rmtG gene ( Table 2) is likely located in the chromosome [62].…”

Section: Rrna Methyltransferases Associated With Aminoglycoside Resismentioning

confidence: 99%

The Role ofPseudomonas aeruginosaRNA Methyltransferases in Antibiotic Resistance

Valderrama-Carmona¹,

Cuartas²,

Castaño³

et al. 2019

Pseudomonas Aeruginosa - An Armory Within

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Methyltransferases play a fundamental role in aminoglycoside resistance of Gram-negative bacteria, and some of its mechanisms were described in the past years, especially in Escherichia coli; however, it remains unsolved for other resistant bacteria such as Pseudomonas aeruginosa. Despite hurdles to determine resistance acquisition, high-throughput approaches (genomics, transcriptomics, and proteomics) have allowed data mining and analysis in a systemic way. Likewise, bioinformatics modelling of homologous genes or proteins has permitted to elucidate the emerging resistance in this pathogen. P. aeruginosa is a bacterial resistance treat since practically all known resistance mechanisms can be described using this model, particularly RNA methyltransferases. The RNA methyltransferases perform methylation or demethylation of ribosomal RNA to allow or restrict the antibiotic resistance development. The Kgm and Kam methyltransferases families are found in P. aeruginosa and confer resistance to several aminoglycosides. Loss of native methylations may also confer a resistant phenotype. The P. aeruginosa RsmG has high structural homology with Thermus aquaticus protein. Today, molecular data will promote a new paradigm on antibiotic therapy for treatment against P. aeruginosa. This chapter provides an overview of what role P. aeruginosa's methyltransferases play in antibiotic resistance, induced by methylation or demethylation in the ribosome.

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RmtC and RmtF 16S rRNA Methyltransferase in NDM-1–ProducingPseudomonas aeruginosa

Cited by 38 publications

References 12 publications

Occurrence of Acquired 16S rRNA Methyltransferase-Mediated Aminoglycoside Resistance in Clinical Isolates of Enterobacteriaceae within a Tertiary Referral Hospital of Northeast India

Occurrence of Acquired 16S rRNA Methyltransferase-Mediated Aminoglycoside Resistance in Clinical Isolates of Enterobacteriaceae within a Tertiary Referral Hospital of Northeast India

Emergence of Multidrug-Resistant Uropathogens harboring ESBL, Carbapenem, Aminoglycosides and AmpC resistant genes from Northern India

The Role ofPseudomonas aeruginosaRNA Methyltransferases in Antibiotic Resistance

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