RNA and Protein Synthesis in Plastid Differentiation

Wollgiehn, R.; Parthier, B.

doi:10.1007/978-3-540-38255-3_4

Cited by 11 publications

(3 citation statements)

References 191 publications

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“…Chloroplast ribosomal proteins L-1 and L-13 were synthesized in vitro from nonpolyadenylated RNA but not from polyadenylated RNA . This seems consistent with the site of synthesis of these two proteins since the alpha, beta, and epsilon subunit polypeptides of CF,, the large subunit of RuBPCase and the 32,000-dalton membrane polypeptide, all products of chloroplast protein synthesis, are known to be translated in vitro from nonpolyadenylated RNA (34,35) . Due to the poor expression of the poly A-RNA under our reaction conditions, we have limited data for these two proteins .…”

Section: Discussionsupporting

confidence: 75%

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Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors.

Schmidt¹,

Myers²,

Gillham³

et al. 1984

The Journal of Cell Biology

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Polyadenylated RNA from Chlamydomonas was translated in a cell-free rabbit reticulocyte system that employed ["S]methionine. Antibodies made to four chloroplast ribosomal proteins synthesized in the cytoplasm and imported into the organelle were used for indirect immunoprecipitation of the labeled translation products, which were subsequently visualized on fluorographs of SDS gels. The cytoplasmically synthesized chloroplast ribosomal proteins were first seen as precursors with apparent molecular weights of 1,000 to 6,000 greater than their respective mature forms. Processing of the ribosomal protein precursors to mature proteins was effected by adding a postribosomal supernatant that had been extracted from cells of Chlamydomonas. In contrast to the chloroplast ribosomal proteins synthesized in the cytoplasm, two such proteins made within the chloroplast were found to be synthesized in mature form in cell-free wheat germ translation systems programmed with nonpolyadenylated RNA.

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Section: Discussionsupporting

confidence: 75%

“…Consequently, immunoprecipitation with anti-L-6 allowed the simultaneous analysis of both proteins L-2 and L-6 in the experiments described below . For reasons that are unclear, protein L-5, known from the 35 S-labeling experiments of Schmidt et al (28) to be made in the cytoplasm, was not observed among the immunoprecipitation products .…”

Section: Resultsmentioning

confidence: 93%

Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors.

Schmidt¹,

Myers²,

Gillham³

et al. 1984

The Journal of Cell Biology

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“…Light itself, therefore, has to be seen as a regulative factor on the effects of the virus. Light does not contain genetic information, but is able to trigger by the photosynthetic apparatus (BUCHANAN 1980) or by photoreceptors (MOHR 1980, ScHOPFER 1977, HARDING and SHROPSHIRE 1980 mediated subsequent reactions which produce the observed after-effects (THOMAS and STODDART 1980, SuNDQVisT et al 1980, WOLLGIEHN and PARTHIER 1980, PARTHIER 1982.…”

Section: Discussionmentioning

confidence: 99%

Seasonal Changes in the Chloroplast Ultrastructure in Abutilon Mosaic Virus (AbMV) infected Abutilon spec. (Malvaceae)

Schuchalter-Eicke

Jeske

1983

Journal of Phytopathology

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Various kinds of pathological effects of the Abutilon Mosaic Virus (AbMV) in seasonal dependency have been observed on the macroscopic as well as on the microscopic level. The annual rhythmic fluctuation of light caused by the varying length and intensity of light incidence is directly correlated with the realization of symptoms, leading to an intensification of the leaf mosaic and to an increase in the light leaf areas during the summer. These macroscopic observations are correlated with an annual change in the plastid fine structure in the mesophyll of the lightest areas of the leaf. The grana‐stroma‐organization of the plastids is completely eliminated through a reduction and disorganization in the thylakoid system during the spring and summer, restored in the autumn, and preserved over the winter. This change in the plastid fine structure is limited to the leaf mesophyll. The plastids of the leaf stem, like the plastids of non‐infected plants, do not display any change in their fine structure.

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Partial characterization of the biosynthesis and integration of the Photosystem II reaction centers in the thylakoid membrane of Chlamydomonas reinhardtii

Delepelaire

1984

The EMBO Journal

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Pulse‐labeling of wild‐type and a Photosystem II mutant strain of Chlamydomonas reinhardtii was carried out in the presence or absence of inhibitors of either cytoplasmic or chloroplast ribosomes, and their thylakoid membrane polypeptides were analyzed by polyacrylamide gel electrophoresis. A pulse‐chase study was also done on the wild‐type strain in the presence of anisomycin, an inhibitor of protein synthesis on cytoplasmic ribosomes. The following results were obtained: the Photosystem II reaction center is mainly composed of integral membrane proteins synthesized within the chloroplast. Several of the proteins of the Photosystem II reaction center are post‐translationally modified, after they have been inserted in the thylakoid membrane.

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RNA and Protein Synthesis in Plastid Differentiation

Cited by 11 publications

References 191 publications

Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors.

Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors.

Seasonal Changes in the Chloroplast Ultrastructure in Abutilon Mosaic Virus (AbMV) infected Abutilon spec. (Malvaceae)

Partial characterization of the biosynthesis and integration of the Photosystem II reaction centers in the thylakoid membrane of Chlamydomonas reinhardtii

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