RNA-Binding Proteins: A Lesson in Repression

Wells, David G.

doi:10.1523/jneurosci.1795-06.2006

Cited by 65 publications

(51 citation statements)

References 42 publications

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“…At this regard, RBPs play a critical role being able to affect the processing of selected transcripts, their transport and localization in the cytoplasm, their stability and translation, thus representing the most important trans-acting factors in the control of gene expression [19,20,51]. The best characterized RBPs are the ELAV proteins that in the genomes of vertebrates include neuron-specific members (HuB, HuC and HuD) and the ubiquitously expressed HuR [52].…”

Section: Discussionmentioning

confidence: 99%

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Amadio¹,

Bucolo²,

Leggio³

et al. 2010

Biochemical Pharmacology

100

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Section: Discussionmentioning

confidence: 99%

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Amadio¹,

Bucolo²,

Leggio³

et al. 2010

Biochemical Pharmacology

100

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“…Given that it is now well established that protein synthesis does occur in axons and synaptic terminals (Hirokawa, 2006;Martin and Zukin, 2006;Schuman et al, 2006;Wells, 2006), it is conceivable that MBNL1 may participate in the transport of mRNAs for some photoreceptor synaptic proteins toward their terminals, much as MBNL2 does in the case of the transport of integrin-␣3 mRNA toward the areas of focal adhesion complexes (Adereth et al, 2005). We are currently investigating this possibility.…”

Section: C) E-jmentioning

confidence: 99%

Developmental regulation of muscleblind‐like (MBNL) gene expression in the chicken embryo retina

Hu¹,

Wahlin²,

McNally³

et al. 2007

Developmental Dynamics

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Muscleblind-like (MBNL) is a CCCH zinc finger-containing RNA-binding protein required for the development of both muscle and photoreceptors in Drosophila; it is conserved evolutionarily, and it is associated in humans with the muscular disease myotonic dystrophy. Its role in the development of vertebrate retinal cells, however, remains unknown. As an initial approach to its investigation, we have cloned three chick muscleblind genes, characterized their isoforms, and examined their expression patterns in the chick embryo retina. The relative levels of expression of the MBNL genes increased during embryonic development. In situ hybridization (ISH) showed that the three MBNL mRNAs had widespread patterns of expression at all the developmental stages examined. Of interest, the temporal and spatial patterns of protein expression, detected by immunocytochemistry with antibodies against MBNL1 and MBNL2, were much more restricted than those seen by ISH. At early stages (ED5-7), for example, MBNL1 and MBNL2 mRNAs were present throughout the retina, but immunoreactivity for the corresponding proteins was largely restricted to the periphery of the optic cup (presumptive iris/ciliary epithelium/ciliary margin zone). MBNL1 and MBNL2 immunoreactivity became detectable at the fundus at later stages, but was limited to a very small subset of the cells that had ISH signals for the cognate mRNAs (particularly ganglion cells and photoreceptors). Within photoreceptors, MBNL1 and MBNL2 immunoreactivity first appeared in their inner segments; MBNL2 remained there, but MBNL1 became subsequently localized to their synaptic terminals. These expression patterns are consistent with the possibility that MBNLs may regulate photoreceptor development in the chick retina, much as MBL does in Drosophila, and suggest that the expression of MBNL1 and MBNL2 may be regulated posttranscriptionally. Developmental Dynamics 237: 286 -296, 2008.

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“…Dendritic targeting elements are found on many dendritically localized mRNAs like MAP2 and CaMKII. 66 Individual RiboNucleoprotein (mRNP) particles formed by association of RNA and binding proteins assemble into larger RNA granules and, in such large complexes, are transported along cytoskeletal tracks by motor proteins enabling their localization. 4,67 b-actin mRNA, which plays an important role in memory associated synaptic plasticity, is present in masked form in resting neurons.…”

Section: Rna Regulation Is Central To Long-term Memory Formationmentioning

confidence: 99%

Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains

Sudhakaran

Ramaswami

2016

RNA Biology

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Long-term and short-term memories differ primarily in the duration of their retention. At a molecular level, long-term memory (LTM) is distinguished from short-term memory (STM) by its requirement for new gene expression. In addition to transcription (nuclear gene expression) the translation of stored mRNAs is necessary for LTM formation. The mechanisms and functions for temporal and spatial regulation of mRNAs required for LTM is a major contemporary problem, of interest from molecular, cell biological, neurobiological and clinical perspectives. This review discusses primary evidence in support for translational regulatory events involved in LTM and a model in which different phases of translation underlie distinct phases of consolidation of memories. However, it focuses largely on mechanisms of memory persistence and the role of prion-like domains in this defining aspect of long-term memory. We consider primary evidence for the concept that Cytoplasmic Polyadenylation Element Binding (CPEB) protein enables the persistence of formed memories by transforming in prion-like manner from a soluble monomeric state to a self-perpetuating and persistent polymeric translationally active state required for maintaining persistent synaptic plasticity. We further discuss prion-like domains prevalent on several other RNA-binding proteins involved in neuronal translational control underlying LTM. Growing evidence indicates that such RNA regulatory proteins are components of mRNP (RiboNucleoProtein) granules. In these proteins, prion-like domains, being intrinsically disordered, could mediate weak transient interactions that allow the assembly of RNP granules, a source of silenced mRNAs whose translation is necessary for LTM. We consider the structural bases for RNA granules formation as well as functions of disordered domains and discuss how these complicate the interpretation of existing experimental data relevant to general mechanisms by which prion-domain containing RBPs function in synapse specific plasticity underlying LTM.

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RNA-Binding Proteins: A Lesson in Repression

Cited by 65 publications

References 42 publications

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Developmental regulation of muscleblind‐like (MBNL) gene expression in the chicken embryo retina

Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains

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