RNA-binding proteins and their role in kidney disease

Seufert, Lisa; Benzing, Thomas; Ignarski, Michael

doi:10.1038/s41581-021-00497-1

Cited by 32 publications

(24 citation statements)

References 248 publications

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“…Concordantly, the m 6 A regulators (YTHDF1, YTHDF2, YTHDC1, METTL3, METTL14, WTAP and ALKBH5) were mostly distributed in the hyper-methylated regions. The prediction of the enrichment of the RBPs associated with kidney disorders ( 40 ) was laid out with IGF2BP2 and MSI1 in the hypermethylated group. Downregulation o f MSI1 can induce tubulointerstitial fibrosis ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

N6-Methyladenosine Methylomic Landscape of Ureteral Deficiency in Reflux Uropathy and Obstructive Uropathy

et al. 2022

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BackgroundCongenital anomalies of the kidneys and urinary tracts (CAKUT) represent the most prevalent cause for renal failure in children. The RNA epigenetic modification N6-methyladenosine (m6A) methylation modulates gene expression and function post-transcriptionally, which has recently been revealed to be critical in organ development. However, it is uncertain whether m6A methylation plays a role in the pathogenesis of CAKUT. Thus, we aimed to explore the pattern of m6A methylation in CAKUT.MethodsUsing m6A-mRNA epitranscriptomic microarray, we investigated the m6A methylomic landscape in the ureter tissue of children with obstructive megaureter (M group) and primary vesicoureteral reflux (V group).ResultsA total of 228 mRNAs engaged in multiple function-relevant signaling pathways were substantially differential methylated between the “V” and “M” groups. Additionally, 215 RNA-binding proteins that recognize differentially methylated regions were predicted based on public databases. The M group showed significantly higher mRNA levels of m6A readers/writers (YTHDF1, YTHDF2, YTHDC1, YTHDC2 and WTAP) and significantly lower mRNA levels of m6A eraser (FTO) according to real-time PCR. To further investigate the differentially methylated genes, m6A methylome and transcriptome data were integrated to identified 298 hypermethylated mRNAs with differential expressions (265 upregulation and 33 downregulation) and 489 hypomethylated mRNAs with differential expressions (431 upregulation and 58 downregulation) in the M/V comparison.ConclusionThe current results highlight the pathogenesis of m6A methylation in obstructive and reflux uropathy.

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Section: Discussionmentioning

confidence: 99%

N6-Methyladenosine Methylomic Landscape of Ureteral Deficiency in Reflux Uropathy and Obstructive Uropathy

et al. 2022

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“…RNA binding proteins (RBPs) are a kind of protein family ubiquitously in eukaryotes and are considered key regulatory components and critical interaction partners for all cellular RNAs (1). In addition to the extensive physiological functions of RBPs, their defective expressions and intracellular mis-localization are contributed to a variety of human diseases, such as virus infection, cancer, aging-related diseases and neurodegenerative diseases (2,3). Generally, RBPs are characterized by the presence of RNA-binding domains (RBDs), through which they bind to target RNAs, thus regulating the fate or function of the bound RNAs.…”

Section: Introductionmentioning

confidence: 99%

How do RNA binding proteins trigger liquid-liquid phase separation in human health and diseases?

Huai

Mao

Wang

et al. 2022

BST

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“…RNA‐binding proteins (RBPs), interacting with multiple RNA species and 100 of individual transcripts, are of fundamental importance for post‐transcriptional gene regulation and protein synthesis 18 . Our previous studies have demonstrated that RCAN1.1 is a novel RNA‐binding protein, binding to 269–291 nt of ANT1 mRNA 15,19 .…”

Section: Introductionmentioning

confidence: 99%

Targeting upregulated RNA binding protein RCAN1.1: a promising strategy for neuroprotection in acute ischemic stroke

et al. 2022

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RNA-binding proteins and their role in kidney disease

Cited by 32 publications

References 248 publications

N6-Methyladenosine Methylomic Landscape of Ureteral Deficiency in Reflux Uropathy and Obstructive Uropathy

N6-Methyladenosine Methylomic Landscape of Ureteral Deficiency in Reflux Uropathy and Obstructive Uropathy

How do RNA binding proteins trigger liquid-liquid phase separation in human health and diseases?

Targeting upregulated RNA binding protein RCAN1.1: a promising strategy for neuroprotection in acute ischemic stroke

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