RNA Dialogues in Fungal–Plant Relationships

Göhre, Vera; Weiberg, Arne

doi:10.1007/978-3-031-16503-0_2

Cited by 4 publications

(3 citation statements)

References 132 publications

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“…Here, we demonstrated that knocking out the BcRDR1 leads to loss of Bc-sRNA production, compromises cross-kingdom RNAi, and reduces pathogenicity. However, BcRDR1 deletion might have affected other endogenous small RNA regulatory processes in the fungus that are relevant for infection [ 30 ]. Therefore, we aimed to further validate that cross-kingdom RNAi was part of B .…”

Section: Resultsmentioning

confidence: 99%

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

Cheng,

Lederer,

Oberkofler

et al. 2023

PLoS Pathog

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Small RNAs act as fungal pathogen effectors that silence host target genes to promote infection, a virulence mechanism termed cross-kingdom RNA interference (RNAi). The essential pathogen factors of cross-kingdom small RNA production are largely unknown. We here characterized the RNA-dependent RNA polymerase (RDR)1 in the fungal plant pathogen Botrytis cinerea that is required for pathogenicity and cross-kingdom RNAi. B. cinerea bcrdr1 knockout (ko) mutants exhibited reduced pathogenicity and loss of cross-kingdom small RNAs. We developed a "switch-on" GFP reporter to study cross-kingdom RNAi in real-time within the living plant tissue which highlighted that bcrdr1 ko mutants were compromised in cross-kingdom RNAi. Moreover, blocking seven pathogen cross-kingdom small RNAs by expressing a short-tandem target mimic RNA in transgenic Arabidopsis thaliana led to reduced infection levels of the fungal pathogen B. cinerea and the oomycete pathogen Hyaloperonospora arabidopsidis. These results demonstrate that cross-kingdom RNAi is significant to promote host infection and making pathogen small RNAs an effective target for crop protection.

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Section: Resultsmentioning

confidence: 99%

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

Cheng,

Lederer,

Oberkofler

et al. 2023

PLoS Pathog

Self Cite

View full text Add to dashboard Cite

show abstract

“…Here, we demonstrate that knocking out the BcRDR1 leads to loss of Bc-sRNA production, compromises cross-kingdom RNAi, and reduces pathogenicity. However, BcRDR1 deletion might have affected other endogenous small RNA regulatory processes in the fungus that are relevant for infection (Göhre & Weiberg, 2022). Therefore, we aimed to further validate that cross-kingdom RNAi was part of B. cinerea pathogenicity.…”

Section: B Cinerea Small Rnas Translocated Into Plants Promote Infectionmentioning

confidence: 99%

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

Cheng

Lederer

Oberkofler

et al. 2023

Preprint

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Small RNAs act as fungal pathogen effectors that silence host target genes to promote infection, a virulence mechanism termed cross-kingdom RNA interference (RNAi). The essential pathogen factors of cross-kingdom small RNA production are largely unknown. We here characterized the RNA-dependent RNA polymerase (RDR)1 in the fungal plant pathogen Botrytis cinerea that is required for pathogenicity and cross-kingdom RNAi. B. cinerea bcrdr1 knockout (ko) mutants exhibited reduced pathogenicity and loss of cross-kingdom small RNAs. We developed a novel "switch-on" GFP reporter to study cross-kingdom RNAi in real-time within the living plant tissue which highlighted that bcrdr1 ko mutants were compromised in cross-kingdom RNAi. Moreover, blocking seven pathogen cross-kingdom small RNAs by expressing a short-tandem target mimic RNA in transgenic Arabidopsis thaliana led to reduced infection levels of the fungal pathogen B. cinerea and the oomycete pathogen Hyaloperonospora arabidopsidis. These results demonstrate that cross-kingdom RNAi is significant to promote host infection and making pathogen small RNAs an effective target for crop protection.

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“…Plant sRNAs can also move intercellularly across plasmodesmata and systemically across the phloem, acting cell non-autonomously and can also translocate between interacting species (Zhan & Meyers, 2023). In the latter case, sRNAs are transferred bidirectionally between plant hosts and pathogenic microbes to modulate defence and virulence, a phenomenon called cross-kingdom RNAi (ckRNAi) (Göhre and Weiberg, 2023; Mahanty et al, 2023; Wang et al, 2017). ckRNAi in plant-pathogen interactions was discovered in 2013 when the necrotrophic fungus Botrytis cinerea was shown to induce silencing of host defence genes in Solanum lycopersicum and Arabidopsis thaliana ( At ) (Weiberg et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A pipeline for validation of sRNA effectors suggests cross-kingdom communication in the symbiosis ofArabidopsiswithSerendipita indica

Nasfi,

Shahbazi,

Bitterlich

et al. 2023

Preprint

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Cross-kingdom RNA interference (ckRNAi), the bidirectional communication between microbes and their host counterparts, is a key element in the outcome of host colonization. Whether mutualistic fungi of the Serendipitaceae family with their broad host range use small RNA effectors (sRNAs) to colonize plant roots is still under debate. To investigate if ckRNAi is a factor in the symbiosis ofSerendipita indica(Si) withArabidopsis thaliana(Ath), we established a pipeline to validate expression, translocation and post-transcriptional gene silencing of host genes bySi-derived sRNAs (SisRNAs). First, we confirmed the expression ofSisRNAs both in axenic fungal culture and duringAthroot colonization using stem-loop PCR. Then, to verify the translocation of putativeSisRNA effectors, an ARGONAUTE 1 immuno-purification assay (AtAGO1-IP) was employed, detecting fungalSisRNAs being loaded into the plant RNAi machinery inSi-colonised roots. Subsequently,SisRNAs and artificial sRNAs (amiRNAs), were transiently expressed inAthprotoplasts to test their gene silencing activity. Stem-loop PCR confirmed expression of sRNA effectors and qPCR validated post-transcriptional gene silencing of their predicted target genes involved in cell wall organization, hormonal signalling regulation, plant immunity and gene expression. Moreover, 5’-RLM-RACE analysis revealed amiRNA-mediated canonical cleavage inArabidopsistargets. In conclusion, this study provides a blueprint for rapid selection and analysis of sRNA effectors in plant-microbe interactions in general and suggests cross-kingdom communication in the Sebacinoid symbiosis.

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RNA Dialogues in Fungal–Plant Relationships

Cited by 4 publications

References 132 publications

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference

A pipeline for validation of sRNA effectors suggests cross-kingdom communication in the symbiosis ofArabidopsiswithSerendipita indica

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