RNA Expression Profiles and Data Mining of Sugarcane Response to Low Temperature

Nogueira, Fábio Tebaldi Silveira; Rosa, Vicente E. De; Menossi, Marcelo; Ulian, E. C.; Arruda, Paulo

doi:10.1104/pp.102.017483

Cited by 163 publications

(126 citation statements)

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“…This is consistent with the inference from the present study, where the ability to maintain photosynthetic rate during chilling correlates with increases in the amount of PPDK. In addition, a stress-induced (drought-induced and cold-induced) increase in PPDK expression has been shown in previous studies of other C4 species (Michalowski et al, 1989;Moons et al, 1998;Nogueira et al, 2003). For example, a RNA profile of cold-responsive genes using filter arrays showed that PPDK transcripts significantly increased on transfer of a sugar cane hybrid (Saccharum sp.…”

Section: Discussionmentioning

confidence: 96%

Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

et al. 2008

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The bioenergy feedstock grass Miscanthus 3 giganteus is exceptional among C 4 species for its high productivity in cold climates. It can maintain photosynthetically active leaves at temperatures 6°C below the minimum for maize (Zea mays), which allows it a longer growing season in cool climates. Understanding the basis for this difference between these two closely related plants may be critical in adapting maize to colder weather. When M. 3 giganteus and maize grown at 25°C were transferred to 14°C, light-saturated CO 2 assimilation and quantum yield of photosystem II declined by 30% and 40%, respectively, in the first 48 h in these two species. The decline continued in maize but arrested and then recovered partially in M. 3 giganteus. Within 24 h of the temperature transition, the pyruvate phosphate dikinase (PPDK) protein content per leaf area transiently declined in M. 3 giganteus but then steadily increased, such that after 7 d the enzyme content was significantly higher than in leaves growing in 25°C. By contrast it declined throughout the chilling period in maize leaves. Rubisco levels remained constant in M. 3 giganteus but declined in maize. Consistent with increased PPDK protein content, the extractable PPDK activity per unit leaf area (V max,ppdk ) in cold-grown M. 3 giganteus leaves was higher than in warm-grown leaves, while V max,ppdk was lower in coldgrown than in warm-grown maize. The rate of light activation of PPDK was also slower in cold-grown maize than M. 3 giganteus. The energy of activation (E a ) of extracted PPDK was lower in cold-grown than warm-grown M. 3 giganteus but not in maize. The specific activities and E a of purified recombinant PPDK from M. 3 giganteus and maize cloned into Escherichia coli were similar. The increase in PPDK protein in the M. 3 giganteus leaves corresponded to an increase in PPDK mRNA level. These results indicate that of the two enzymes known to limit C 4 photosynthesis, increase of PPDK, not Rubisco content, corresponds to the recovery and maintenance of photosynthetic capacity. Functionally, increased enzyme concentration is shown to increase stability of M. 3 giganteus PPDK at low temperature. The results suggest that increases in either PPDK RNA transcription and/or the stability of this RNA are important for the increase in PPDK protein content and activity in M. 3 giganteus under chilling conditions relative to maize.

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Section: Discussionmentioning

confidence: 96%

Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

et al. 2008

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“…In the case of Arabidopsis, a dicot model, it was revealed a down-regulated expression pattern promoted by high-salinity stress for AtUCP4 and AtUCP5 ( S EKI et al, 2002). However, other abiotic stresses as low and high temperatures (NANTES et al, 1999;NOGUEIRA et al, 2003;SWIDZINSKI et al, 2002) drought, wound (CHEONG et al, 2002 induced up-regulation in AtUCP genes. These results are puzzling but it could be suggested a differential regulation at cell or tissue/organ level among different species.…”

Section: Resultsmentioning

confidence: 99%

“…Salinity, as several others biotic and abiotic stresses, alters the equilibrium between the production and the scavenging of ROS (GILL; TUTEJA, 2010; MUNNS; TESTER, 2008;PANG;WANG, 2008;PARIDA;DAS, 2005). ROS are produced continuously in different cellular compartments such as chloroplast, mitochondria and peroxisomes and their imbalance cause oxidative damage to membrane lipids, proteins, and nucleic acids (ASHRAF, 2009;BANU et al, 2009;JEZEK;HLAVATÁ, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…Up to date, apparently, there is no study with cowpea pUCPs in response to salt stress. These proteins are encoded by a multigene family and are related to plant adjustment mechanisms under environmental stresses (BORECKÝ et al, 2006;CHEONG et al, 2002;NANTES et al, 1999;NOGUEIRA et al, 2003;SEKI et al, 2002;SWIDZINSKI et al, 2002). Two pUCP genes were identified in cowpea: VuUCP1a a n d VuUCP1b (GARANTIZADO, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the salinity imposes two stresses on plant tissue: a water-deficit that results from the relatively high solute concentrations in the soil, and ion toxicity due altered K/Na rations and Na + and Cl -concentrations that are harmful to plants (ALVES et al, 2008;CHEN et al, 2008). Salt stress affects almost every aspect of the physiology and biochemistry of plants, promoting ionic, osmotic, nutritional and hormonal imbalances, leading to a decrease in metabolic efficiency and even to injurious secondary effects such as oxidative stress promoted by reactive oxygen species (ROS) (MUNNS; TESTER, 2008;PANG;WANG, 2008;PARIDA;DAS, 2005). ROS are produced predominantly in chloroplasts, mitochondria, and peroxisomes causing oxidative damage to membrane lipids, proteins, and nucleic acids (ASHRAF, 2009;BANU et al, 2009;JEZEK;HLAVATÁ, 2005) and can induce responses such as new gene expression (GILL; TUTEJA, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Expressão diferencial dos genes VuUCP1a e VuUCP1b em caupi sob estresse salino

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Maia³

et al. 2011

Rev. Ciênc. Agron.

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Expressão diferencial dos genesResumo -O estresse salino afeta o crescimento e o desenvolvimento das plantas, induzindo respostas bioquímicas e fisiológicas como mecanismo de adaptação para sua sobrevivência. As proteínas desacopladoras de planta (pUCPs) dissipam o gradiente eletroquímico mitocondrial de prótons como calor e são codificadas por famílias multigênicas. Elas atuam como sistema de defesa evitando a formação de espécies reativas de oxigênio geradas em resposta a estresses ambientais. O objetivo do trabalho foi estudar a expressão de genes das pUCPs (VuUCP1a e VuUCP1b ) em raízes e folhas de plântulas de Vigna unguiculata submetidas ao estresse salino (NaCl 100 mM). Sementes foram germinadas no escuro e após 3 dias, as plântulas foram transferidas para solução de Hoagland, permanecendo 3 dias antes da aplicação do estresse. As raízes e folhas foram coletadas com 0; 6; 12 e 24 horas, após adição de NaCl, para avaliar o perfil de expressão dos genes da pUCP por RT-PCR. A análise de transcritos mostrou que VuUCP1a é expresso em raízes e folhas revelando um perfil constitutivo enquanto que VuUCP1b é dependente do tecido, isto é, expresso nas folhas, sem alteração em resposta ao estresse e induzido pelo estresse salino nas raízes. A peculiaridade da duplicação gênica de pUCP1 em caupi com perfil diferencial de expressão, sugere tanto um papel dessa enzima nos mecanismos de ajustamento ao estresse salino quanto promove essa espécie como um modelo atrativo para compreensão do papel da família multigênica da pUCP em plantas. Palavras-chaves -Estresse salino. pUCP. Vigna unguiculata.Abstract -Salt stress affects growth and development of plants, inducing a variety of physiological and biochemical responses as an adaptation mechanism for survival. The plant uncoupling mitochondrial proteins (pUCPs) are able to dissipate the proton electrochemical gradient as heat and are encoded by a multigene family. They works as defence systems avoiding the formation of reactive oxygen species promoted by environmental stress. The aim of this work was to study gene expression of pUCPs (VuUCP1a and VuUCP1b) in roots and leaves from Vigna unguiculata seedlings under salt stress (100 mM NaCl). Seeds were germinated in the dark and after 3 days, the seedlings were transferred to Hoagland's medium and grown for 3 additional days before being submitted to the stress condition. Roots and leaves were harvested at 0; 6; 12 and 24 hours after addition of NaCl for total RNA isolation and RT-PCR assays. Expression analysis by RT-PCR showed that VuUCP1a is constitutive in leaves and roots while VuUCP1b is expressed as tissue-dependent presenting a constitutive profile in leaves and a differential one in roots from seedlings under salt stress. The uniqueness of pUCP1 gene duplication in cowpea with differential expression suggest a role of this enzyme in the adjustment of salt stress as well as promotes this species as an attractive model to understand the role of pUCP gene members in plants.

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