RNA helicases: regulators of differentiation

Abdelhaleem, Mohamed

doi:10.1016/j.clinbiochem.2005.01.010

Cited by 111 publications

(91 citation statements)

References 67 publications

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“…DBY is located in the non-recombining region of the Y chromosome (Yq11), belongs to the DEAD box proteins and encodes a putative ATP-dependent RNA helicase [20]. The DEAD-box proteins are implicated in a number of cellular processes involving alterations of the secondary structure of RNA, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.…”

Section: Discussionmentioning

confidence: 99%

The role of Dby mRNA in early development of male mouse zygotes

Yao¹,

Xu²,

Gong³

et al. 2010

Asian J Androl

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Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location of Dby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group (35.9% vs. 95%, P = 0.001) and the as-Dby female pronucleus injection group (35.9% vs. 93.8%, P = 0.001). The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group (17.4% vs. 57.9%, P = 0.002) and the as-Dby female pronucleus injection group (17.4% vs. 54.1%, P = 0.002). Thus, we conclude that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.

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Section: Discussionmentioning

confidence: 99%

The role of Dby mRNA in early development of male mouse zygotes

Yao¹,

Xu²,

Gong³

et al. 2010

Asian J Androl

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“…DEAD box RNA helicases have been shown to function in RNA metabolism including translation, ribosome biogenesis, pre-mRNA splicing and nucleo-cytoplasmic RNA transport (Cordin et al, 2006;Linder, 2006). Besides their role in RNA biogenesis there is now evidence to indicate that DEAD box RNA helicases can participate in the progression of cancer (Abdelhaleem, 2005). For example, human DDX5 has been shown to be overexpressed at the protein level in colorectal tumors as well as in the hepatic tumor cell lines, HT-29 and HCT116, which have undergone an epithelial-mesenchymal transition (Yang et al, 2005(Yang et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Oncogenic role of DDX3 in breast cancer biogenesis

et al. 2008

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Benzo [a]pyrene diol epoxide (BPDE), the active metabolite of benzo [a]pyrene present in tobacco smoke, is a major cancer-causing compound. To evaluate the effects of BPDE on human breast epithelial cells, we exposed an immortalized human breast cell line, MCF 10A, to BPDE and characterized the gene expression pattern. Of the differential genes expressed, we found consistent activation of DDX3, a member of the DEAD box RNA helicase family. Overexpression of DDX3 in MCF 10A cells induced an epithelial-mesenchymal-like transformation, exhibited increased motility and invasive properties, and formed colonies in soft-agar assays. Besides the altered phenotype, MCF 10A-DDX3 cells repressed E-cadherin expression as demonstrated by both immunoblots and by E-cadherin promoter-reporter assays. In addition, an in vivo association of DDX3 and the E-cadherin promoter was demonstrated by chromatin immunoprecipitation assays. Collectively, these results demonstrate that the activation of DDX3 by BPDE, can promote growth, proliferation and neoplastic transformation of breast epithelial cells.

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“…A variety of RNA metabolic processes, including transcription, ribosomal biogenesis, RNA splicing, editing, transport and translational events, are also controlled by VASA. [16][17][18][19][20][21] In addition, recent studies have shown VASA gene involvement in RNA metabolism and micro RNA processing. 22 The aim of this study was to test the possibility that the DNA methylation states in the VASA promoter CpG islands may contribute to some forms of human idiopathic male infertility.…”

Section: Introductionmentioning

confidence: 99%