RNA interference- a novel approach for plant disease management

Puyam, Anita; Sharma, Sucheta; Kashyap, Prem Lal

doi:10.31018/jans.v9i3.1410

Cited by 11 publications

(2 citation statements)

References 61 publications

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“…RNAi is a natural biological process that can be harnessed to silence specific genes in pests and pathogens, including rust-causing fungi (Panwar et al, 2018). By using RNAi, researchers can inhibit the expression of rust pathogen genes and reduce disease severity in plants (Puyam et al, 2017). RNAi is a powerful and natural biological process that has been harnessed for its potential in developing rust-resistant crops (Halder et al, 2022).…”

Section: Rna Interference (Rnai)mentioning

confidence: 99%

Modern Approaches to Enhancing Rust Resistance in Wheat Leading to Global Food Security

Afzal,

Mushtaq,

Ahmad

et al. 2024

Plant Prot.

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Rust diseases pose significant threats to wheat production. The deployment of wheat cultivars endowed with rust resistance stands as the most potent strategy for effective rust management. This resistance is primarily inherited through Mendelian principles discovered in 1905, but traditional breeding methods are time-consuming. Modern strategies have emerged to develop rust-resistant wheat varieties efficiently. Marker-Assisted Selection (MAS) accelerates the breeding process through precise screening, bringing about a revolution in the creation of rust-resistant wheat varieties. Genetic engineering techniques allow the transfer of resistance genes from other species into susceptible crops, but GMO use remains controversial and regulated. Gene editing, especially with CRISPR-Cas9, is a game-changer, enabling the introduction of natural variations or inactivation of critical genes in rust pathogens, enhancing plant resistance. RNA interference (RNAi) is another promising strategy, using small RNA molecules to inhibit rust pathogen gene expression, reducing disease severity. Induced Systemic Resistance (ISR) primes plant immune systems by treating them with beneficial microorganisms or compounds, fortifying them against subsequent rust infections. Eco-friendly biofungicides with antagonistic microorganisms suppress rust infections as alternatives to chemical fungicides. The development of climate-resilient wheat varieties is essential, as they indirectly enhance rust resistance, ensuring stable production amid changing climate conditions. These efforts to improve wheat productivity and rust resistance are crucial for feeding the growing global population. Integrating modern methods with traditional breeding is key to effectively combatting rust diseases and enhancing food security.

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Section: Rna Interference (Rnai)mentioning

confidence: 99%

Modern Approaches to Enhancing Rust Resistance in Wheat Leading to Global Food Security

Afzal,

Mushtaq,

Ahmad

et al. 2024

Plant Prot.

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“…RNAi regulates a variety of biological processes, including plant immunity [5], and siRNAs and miRNAs have been identified as key factors in plant defense against viruses, bacteria, and fungi [6][7][8][9]. As shown in Figure 1, the silencing process starts with the binding of a host's ribonuclease-III called Dicer (DICER) to long dsRNAs or hpRNA and their cleavage into siRNAs of 21-25 nt in length [10,11]. DICER has a helicase domain, a Piwi/Argonaute/Zwille (PAZ) motif, a dsRNA binding domain at the N-terminus, and two RNase III motifs at the C-terminus.…”

Section: Introductionmentioning

confidence: 99%

RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi

Padilla-Roji,

Ruiz-Jiménez,

Bakhat

et al. 2023

IJMS

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Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.

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